Product Details

Purity

>85% (SDS-PAGE)

Target Names

ORF1

Uniprot No.

Q81862

Alternative Names

ORF1; Non-structural polyprotein pORF1 [Includes: Methyltransferase; EC 2.1.1.-); Papain-like cysteine protease; PLP; EC 3.4.22.-); NTPase/helicase; EC 3.6.4.-); RNA-directed RNA polymerase; RdRp; EC 2.7.7.48)]

Species

Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Methyltransferase: Displays a capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m(7)GTP or m(7)GDP. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m(7)GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure.; Papain-like cysteine protease: May participate in the processing of polyprotein pORF1 together with cellular proteases and the cleavage of capsid protein ORF2.; NTPase/helicase: Multi-functional protein that exhibits NTPase and RNA unwinding activities. Hydrolyzes all NTPs efficiently and unwinds RNA duplexes containing 5' overhangs. Possesses a sequence independent RNA-5'-triphosphatase (RTPase) activity suggestive of its role in forming viral cap structure. Participates also in viral genome replication, RNA translocation and genome packaging/unpackaging.; RNA-directed RNA polymerase: Plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA to initiate viral replication.

Gene References into Functions

this study demonstrated that thrombin and factor Xa cleavage sites on HEV pORF1 are obligatory for HEV replication. PMID: 29321328
the D29N and V27A mutations in the methyltransferase region had significant association with the poor outcome in acute liver failure patients suggesting key role in enhancing HEV replication PMID: 28359977
Here, we report that the HEV X-domain directly interacts with the viral methyltransferase and the ORF3 proteins. PMID: 27113483
Y-domain sequences have a critical role in the HEV life cycle, which may involve gene regulation and/or membrane binding in intracellular replication complexes PMID: 28216965
In the case of hepatitis E virus, the adjacent viral helicase domain dramatically increases the binding of the macro domain to poly(ADP-ribose) and simulates the demodification activity. PMID: 27440879
Mutational analysis of the conserved serine/cysteine/histidine residues suggested that H443 and C472/C481/C483 are possibly the active site residues. PMID: 24795447
Motifs Ia and III of helicase are critical for positive sense RNA virus replication. PMID: 24333153
Cys434-His443 is the catalytic site of papain-like cysteine protease. PMID: 24321124
Collectively, the indispensability and functional effects of 'PCP-catalytic' and 'X-substrate' residues on HEV replication strongly supported a viral protease. PMID: 23978667
Hence, great quasispecies heterogeneity in the regions encoding the polyproline region and the macro domain may facilitate hepatitis E virus persistence. PMID: 23964111
The methyltransferase-papain-like cysteine protease domain of ORF1 carried out deISGylation of the ISG15-conjugated cellular proteins, suggesting a possible role in combating cellular antiviral pathways. PMID: 21653754
When incubated with 5'-[gamma-(32)P]RNA and 5'-[alpha-(32)P]RNA, hepatitis E virus helicase released (32)P only from 5'-[gamma-(32)P]RNA, showing specificity for the gamma-beta-triphosphate bond. PMID: 20592074

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Subcellular Location

Host cytoplasm.

Protein Families

Hepevirus non-structural polyprotein family

Database Links

KEGG: vg:1494415

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Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 (ORF1), partial

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