Recombinant Human Acetyl-CoA carboxylase 2(ACACB) ,partial

Code CSB-YP001120HU
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Source Yeast
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Code CSB-EP001120HU
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Source E.coli
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Code CSB-EP001120HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001120HU
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Source Baculovirus
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Code CSB-MP001120HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ACACB
Uniprot No. O00763
Alternative Names ACACB; ACACB_HUMAN; ACC-beta; ACC2; ACCB; Acetyl CoA carboxylase beta; Biotin carboxylase; HACC275
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation. Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage.
Gene References into Functions
  1. ACC2 gene (ACACB) expression was decreased by 25% in HCC tissue compared to non-cancerous liver tissue. PMID: 28290443
  2. PHD3 loss in cancer enables metabolic reliance on fatty acid oxidation via deactivation of ACC2. PMID: 27635760
  3. A significant association exists of ACACB gene polymorphism and diabetic nephropathy among Caucasian patients with diabetes. PMID: 26030797
  4. Our meta-analysis supports that the apolipoprotein E epsilon2 allele and acetyl-CoA carboxylase beta rs2268388 C>T might act as promotion factors of nephropathy in type 2 diabetes. PMID: 25262148
  5. The knockdown of ACC2 reduced palmitic acid -induced autophagy and thus protects the cells from palmitic acid - induced lipotoxicity with attenuated lipid accumulation and rescued cell viability. PMID: 26022126
  6. These data support a role for ACACB in obesity and potential roles for altered lipid metabolism in susceptibility to diabetic nephropathy. PMID: 23460794
  7. TT genotypes of ACACB gene (rs2268388) and CC genotype of AGTR1 gene (rs5186) confers the risk of diabetic nephropathy in Asian Indian patients with T2DM. PMID: 23081748
  8. Its involvement in the development of diabetic nephropathy is explained by the promotion of the so-called micro-inflammation associated with the diabetic state. PMID: 23156397
  9. A gene polymorphism in acetyl-coenzyme A carboxylase beta may be associated with the C-reactive protein level in a prediabetic and diabetic population. PMID: 21553357
  10. In conclusion, common polymorphisms of ACACB gene are associated with obesity and, independently, with type 2 diabetes in postmenopausal women PMID: 21908218
  11. Common variants within the ACACB locus appear to regulate adipose gene expression PMID: 21887335
  12. Structure-guided inhibitor design for human acetyl-coenzyme A carboxylase by interspecies active site conversion. PMID: 21953464
  13. The acetyl-coenzyme A carboxylase beta (ACACB) gene is associated with nephropathy in Chinese patients with type 2 diabetes. PMID: 20519229
  14. Acetyl-CoA carboxylase beta (ACC2) plays a key role in fatty acid synthesis and oxidation pathways. PMID: 20855566
  15. The -368 C/T single-nucleotide polymorphism in ACACB P-II binds HepG2 nuclear proteins that affect promoter activity in an allele-specific fashion. PMID: 20799892
  16. ACACB overexpression in renal proximal tubular epithelial cell increased proinflammatory cytokine expression, such as IL-6, at least partly by increasing mRNA stability through a p38 MAPK-dependent pathway. PMID: 20514549
  17. This study demonstrates that NRF-1 is a novel transcriptional inhibitor of the human ACCbeta gene promoter in the mammalian heart. PMID: 20599696
  18. These results suggest that ACACB is a strong candidate for conferring susceptibility for proteinuria in patients with type 2 diabetes. PMID: 20168990
  19. the crystal structures of the biotin carboxylase domain of human ACC2 phosphorylated by AMP-activated protein kinase was reported. PMID: 19900410
  20. The effect of a 3-month low-intensity endurance training program on fat oxidation and expression PMID: 12086953
  21. elevation of AMPK via phosphorylation is not sufficient to maintain elevated ACCbeta Ser(221) phosphorylation during exercise PMID: 12413941
  22. down-regulation of acetyl coa carboxylase 2 (ACC2) mRNA, induced by the lowering of plasma insulin concentration, is related to improvement of insulin sensitivity PMID: 14627750
  23. differential regulation of ACCbeta gene expression between tissues PMID: 15590647
  24. The expression, purification, and characterization of ACC2 were investigated. PMID: 17223360
  25. Human adipose tissue, unlike rodent adipose, expresses more ACC2 mRNA relative to the oxidative tissues muscle and heart. PMID: 19190759
  26. The human ACC2 CT-domain C-terminus is comprised of three intertwined alpha-helices that extend outwards from the enzyme on the opposite side to the ligand-binding site. PMID: 19390150
  27. results point towards major differences in ACC tissue distribution between humans and rats PMID: 19618481

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Subcellular Location Mitochondrion.
Tissue Specificity Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon. Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level). Isoform 3 is detected at high levels in
Database Links

HGNC: 85

OMIM: 601557

KEGG: hsa:32

STRING: 9606.ENSP00000341044

UniGene: Hs.234898

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