Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cardiovascular
Alternative Names
AMDM; ANP-B; ANPB; ANPR-B; ANPRB; ANPRB_HUMAN; Atrial natriuretic peptide B type receptor; Atrial natriuretic peptide receptor 2; Atrial natriuretic peptide receptor type B; Atrionatriuretic peptide receptor B; GC-B; GCB; Guanylate cyclase B; GUC 2B; GUC2B; GUCY2B; Natriuretic peptide receptor B; Natriuretic peptide receptor B/guanylate cyclase B; NPR-B; Npr2; NPRB; NPRBi
Species
Homo sapiens (Human)
Expression Region
23-458aa
Target Protein Sequence
RNLTLAVVLPEHNLSYAWAWPRVGPAVALAVEALGRALPVDLRFVSSELEGACSEYLAPLSAVDLKLYHDPDLLLGPGCVYPAASVARFASHWRLPLLTAGAVASGFSAKNDHYRTLVRTGPSAPKLGEFVVTLHGHFNWTARAALLYLDARTDDRPHYFTIEGVFEALQGSNLSVQHQVYAREPGGPEQATHFIRANGRIVYICGPLEMLHEILLQAQRENLTNGDYVFFYLDVFGESLRAGPTRATGRPWQDNRTREQAQALREAFQTVLVITYREPPNPEYQEFQNRLLIRAREDFGVELGPSLMNLIAGCFYDGILLYAEVLNETIQEGGTREDGLRIVEKMQGRRYHGVTGLVVMDKNNDRETDFVLWAMGDLDSGDFQPAAHYSGAEKQIWWTGRPIPWVKGAPPSDNPPCAFDLDDPSCDKTPLSTLAI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human NPR2 was expressed with the amino acid range of 23-458. This NPR2 protein is theoretically predicted to have a molecular weight of 64.5 kDa. The NPR2 protein was expressed in e.coli. The NPR2 coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant NPR2 protein in following stages of expression and purification.
Atrial natriuretic peptide receptor 2 (NPR2) is a membrane-bound guanylate cyclase receptor that plays a crucial role in cardiovascular regulation. Also known as natriuretic peptide receptor B (NPR-B), it primarily binds atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). Upon binding, NPR2 activates the synthesis of cyclic GMP (cGMP), a second messenger that regulates various physiological processes. NPR2 is particularly important in the regulation of blood pressure, fluid homeostasis, and cardiovascular development. Mutations in the NPR2 gene are associated with certain skeletal dysplasias. Research on NPR2 extends into cardiovascular biology, endocrinology, and the development of therapeutic strategies targeting the natriuretic peptide pathway.