NPR2

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate
All lanes: ANPRB antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa

Immunofluorescence staining of Hela with CSB-RA926985A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 501-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA926985A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA978547(NPR2 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA978547(NPR2 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue, Primary antibody: CSB-PA978547(NPR2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds