Recombinant Human BDNF/NT-3 growth factors receptor(NTRK2),partial

Code CSB-YP619082HU
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Source Yeast
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Code CSB-EP619082HU
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Source E.coli
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Code CSB-EP619082HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619082HU
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Source Baculovirus
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Code CSB-MP619082HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NTRK2
Uniprot No. Q16620
Alternative Names AI848316; BDNF tropomyosine receptor kinase B; BDNF/NT 3 growth factors receptor; BDNF/NT-3 growth factors receptor; Brain derived neurotrophic factor receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP95 TrkB; Neurotrophic receptor tyrosine kinase 2; Neurotrophic tyrosine kinase receptor type 2; Neurotrophin receptor tyrosine kinase type 2; NTRK 2; Ntrk2; NTRK2_HUMAN; Obesity, hyperphagia, and developmental delay, included; RATTRKB1; Tkrb; Trk B; Trk-B; TRKB; TrkB tyrosine kinase; TRKB1; Tropomyosin related kinase B; tyrosine kinase receptor B; Tyrosine receptor kinase B
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
Gene References into Functions
  1. MNX1 may directly regulate TrkB expression, which may increase their metastatic potential via suppression of anoikis and enhanced adhesion to the ECM. PMID: 30066929
  2. TRKB role in epithelial-mesenchymal transition, migration and invasion of cervical cancer. PMID: 29345295
  3. BDNF/TrkB axis plays a role in epithelial mesenchymal transition promoting the acquisition of (myo)fibroblast cell phenotype in idiopathic pulmonary fibrosis. PMID: 28938915
  4. Low TRKB expression is associated with Parkinson's disease. PMID: 28923922
  5. Study observed that patients with epilepsy might have a different variant distribution in the NTRK2 gene compared to controls without epilepsy, and that NTRK2 variability might influence age at seizure onset and possibly seizure control. PMID: 28863320
  6. Study reports that cells residing in the human subependymal zone (SEZ) may maintain their responsiveness to mature BDNF, although this responsiveness seems to undergo age-related alterations. Results indicate that TrkB-TK+ expression may promote neuronal differentiation, whereas TrkB-TK- expression may block neuronal differentiation in favour of neural precursor proliferation and/or glial differentiation. PMID: 28612959
  7. these findings demonstrate that TrkB-mediated signaling contributes to the induction of malignant phenotypes (proliferation, invasiveness, angiogenesis, lymphangiogenesis, and tumorigenesis) in gallbladder cancer PMID: 28423707
  8. our findings suggest that heterodimerization of Her2 and TrkB receptors gives breast cancer cells a survival advantage in the brain and that dual inhibition of these receptors may hold therapeutic potential PMID: 28446206
  9. TrkB-containing exosomes play a key role in the control of glioblastoma progression and aggressiveness, as seen in a model of YKL-40-inactivated glioblastoma cells PMID: 27385098
  10. Female sex and specific allelic variations in the NTRK2 gene were independently associated with mood disorders or depressive disorders in temporal lobe epilepsy patients. PMID: 28550723
  11. Report an association between antidepressant worsening suicidal ideation and SNP rs1439050 of NTRK2 in depressed subjects treated by tianeptine. PMID: 27378793
  12. This work demonstrates that DHF, through activation of TrkB signaling pathway, has a preventive function in d-glucose-induced apoptosis in PRE cells in diabetic retinopathy. PMID: 29109000
  13. The aim of the current review was to provide an overview of the role of BDNF/TrkB pathways in the pathogenesis of breast cancer and its value as a potential therapeutic target. PMID: 28230291
  14. Data suggest that high expression of TRKB (not TRKA or TRKC) in lung squamous cell carcinoma is correlated with vascular invasion, lymph node metastasis, advanced stage of tumor, and poor prognosis; overall survival of patients with TRKB-high expression is significantly shorter than those with TRKB-low expression; RNA interference against TRKB or BDNF (TRKB agonist) inhibits cell proliferation of tumor cell line. PMID: 28604655
  15. Among the adaptations observed following consumption of drugs of abuse or alcohol abuse, BDNF levels are widely altered in both brain and periphery. Data suggest that BDNF/TRKB signaling in neurons in specific areas of brain (hippocampus, nucleus accumbens) is altered in protracted withdrawal from drug/alcohol abuse. [REVIEW] PMID: 28215305
  16. High TRKB expression is associated with Small Cell Lung Cancer. PMID: 28870922
  17. High NTRK2 expression is associated with astrocytomas. PMID: 27402815
  18. overexpression of TrkB, but not of BDNF, is significantly associated with a poor survival outcome for TNBC patients. PMID: 28604807
  19. In the current work, we provide a global analysis of the physiological consequences of TrkB receptor activation in vitro and discuss the dynamic consequences of TrkB activation in vivo. Finally, we propose a clinically-feasible option for increasing BDNF expression in the hypothalamus to more readily utilize the oncolytic effects of BDNF. PMID: 28739680
  20. These findings, while exploratory, indicate that the genotypes of NTRK2 and COMT may contribute to relative risk for symptom burden during and shortly after the period of chemotherapy in women with early stage breast cancer. PMID: 28205449
  21. We demonstrated that downregulation of PHLDA1 leads to a significant increase in expression of Aurora A and TRKB that are markers of poor prognosis in neuroblastoma PMID: 27278006
  22. The receptors, TrkB and p75 are more highly expressed in deep infiltrating endometriosis than in peritoneal tissues. PMID: 27519317
  23. High expression of NTRK2 is associated with gastric cancer. PMID: 27662840
  24. Genetic variability of NTRK2, a susceptibility gene for psychiatric disorders, is related to emotional arousal and-independently-to brain white-matter properties in healthy individuals. PMID: 26978740
  25. High expression of TrkA, TrkB, or TrkC was significantly associated with histopathology PMID: 26459250
  26. Results show up-regulation of TrkB and down-regulation of Runx3 and Keap1 in breast cancer cells and suggest that TrkB plays a key role in tumorigenicity and metastasis of breast cancer cells through suppression of Runx3 or Keap1. PMID: 26657794
  27. Our data revealed that anoikis-resistant ACHN cells presented with tolerance to detachment-induced apoptosis, excessive proliferation and aggressive invasion, accompanied by upregulation of TrkB expression in contrast to parental cells PMID: 26820170
  28. results demonstrate the occurrence of ASIC2 and TrkB in the human intervertebral disc (IVD), and the increased expression of both in pathological IVD suggest their involvement in IVD degeneration PMID: 26617738
  29. Data indicate the role of tropomyosin-related kinase-B (TrkB) in activation of the interleukin-6/Janus kinase 2/STAT3 transcription factor and PI3 kinase/c-AKT proto-oncogene protein pathway in breast cancer. PMID: 26515594
  30. soluble Abeta42 and BDNF, but not TrkB or soluble tau, correlate with dementia in the oldest-old PMID: 26410307
  31. The rare variants in NTRK2 were significantly associated with smoking status. PMID: 25450229
  32. Entrectinib inhibits growth of TrkB expressing neuroblastoma cells in vitro and in vivo, and that it enhances the efficacy of conventional chemotherapy in in vivo models. PMID: 26797418
  33. Single-nucleotide polymorphisms rs1778929 and rs1187323 in the TrkB gene are significantly associated with post-stroke depression in the Chinese population. PMID: 26641254
  34. TrkB protein was mainly expressed in eutopic endometrium of proliferative phase with EMs than that in eutopic endometrium of secretory phase PMID: 27048022
  35. Results highlight the importance of brain-derived neurotrophic factor-induced tropomyosin receptor kinase B receptor signaling in rescuing Huntington's disease-mediated apoptotic features in striatal cells. PMID: 25896770
  36. Demonstrate that TrkB protects endothelial integrity during atherogenesis by promoting Ets1-mediated VE-cadherin expression and plays a previously unknown protective role in the development of coronary artery disease. PMID: 25633318
  37. Excessive activation of TrkB was found to promote epilepsy in a transgenic mouse model. PMID: 26481038
  38. This study demonstrated that TrkB expression in brain in patient with schizophrenia, bipolar disorder, and major depressive disorder. PMID: 25796564
  39. Our study showed that AZD6918 induced cell death as a single agent and attenuated BDNF/TrkB-induced protection from etoposide in vitro. PMID: 25700942
  40. Our results suggest that genetic variability in FKBP5 and NTRK2 genes may partially explain clinical response to clozapine PMID: 25751398
  41. Membranous/cytoplasmic TrkB may promote an epithelial-mesenchymal transition (EMT)-like phenotype with high-grade budding and maintain viability of buds themselves. PMID: 25382057
  42. The role of NTRK2 in in paranoid schizophrenia development in Russians. PMID: 26410934
  43. TrkB expression in lung adenoma cells is an early step in tumor cell dissemination. PMID: 24959744
  44. mRNA expression of NTRK2 genes was higher in low-grade gliomas vs. high-grade and control samples. Poor survival was associated with NTRK2 mRNA. Promoter methylation does not regulate NTRK2 genes in glioma. PMID: 24840578
  45. these results reinforce the role of NTRK2 gene in the pathogenesis of suicide. PMID: 25110312
  46. study demonstrated that the BDNF/TrkB axis promotes the migration and invasion of SACC cells via EMT in vitro PMID: 25456007
  47. Results present evidence of a role for BBS4 in mediating the phosphorylation of TrkB by BDNF and its activation requires a proper localization to the ciliary axoneme. PMID: 24867303
  48. Site-specific differential methylation in the TrkB-T1 3'UTR region associates with functional changes in TrkB-T1 expression and may play a significant role in the important decrease of cortical TrkB-T1 expression observed among suicide completers. PMID: 24802768
  49. TrkB-EGFR-sortilin (TES) complex in exosomes has a function in the activation and migration of endothelial cells. PMID: 25037567
  50. BDNF and TrkB are involved in vessel formation and osteogenic processes during human fracture healing PMID: 24984919

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Involvement in disease Obesity, hyperphagia, and developmental delay (OBHD)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Endosome membrane, Single-pass type I membrane protein, Early endosome membrane
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Tissue Specificity Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cor
Database Links

HGNC: 8032

OMIM: 600456

KEGG: hsa:4915

STRING: 9606.ENSP00000277120

UniGene: Hs.494312

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