Recombinant Human Carboxypeptidase N catalytic chain (CPN1)

Code CSB-YP005898HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005898HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP005898HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CPN1
Uniprot No.
Alternative Names
ACBP; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N catalytic chain; Carboxypeptidase N polypeptide 1 50 kD; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Carboxypeptidase N subunit 1; CBPN_HUMAN; CPN; CPN1; Kininase 1; Kininase-1; Kininase1; Lysine carboxypeptidase; Plasma carboxypeptidase B; SCPN; Serum carboxypeptidase N
Species
Homo sapiens (Human)
Expression Region
21-458
Target Protein Sequence
VTFRHHRYDD LVRTLYKVQN ECPGITRVYS IGRSVEGRHL YVLEFSDHPG IHEPLEPEVK YVGNMHGNEA LGRELMLQLS EFLCEEFRNR NQRIVQLIQD TRIHILPSMN PDGYEVAAAQ GPNKPGYLVG RNNANGVDLN RNFPDLNTYI YYNEKYGGPN HHLPLPDNWK SQVEPETRAV IRWMHSFNFV LSANLHGGAV VANYPYDKSF EHRVRGVRRT ASTPTPDDKL FQKLAKVYSY AHGWMFQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT NCFEITLELS CDKFPPEEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN YNNLANAVIS VSGINHDVTS GDHGDYFRLL LPGIYTVSAT APGYDPETVT VTVGPAEPTL VNFHLKRSIP QVSPVRRAPS RRHGVRAKVQ PQARKKEMEM RQLQRGPA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Gene References into Functions
  1. CPN binds to fibrinogen and is present in a fibrin clot prepared from plasma. PMID: 23000409
  2. carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity PMID: 15718415
  3. This review summarizes the structure, enzymatic properties and function of this enzyme, including insights gained by the recent elucidation of the crystal structure of the CPN catalytic subunit and structural modeling of the non-catalytic 83 kDa subunit. PMID: 18039526
Involvement in disease
Carboxypeptidase N deficiency (CPND)
Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase M14 family
Tissue Specificity
Synthesized in the liver and secreted in plasma.
Database Links

HGNC: 2312

OMIM: 212070

KEGG: hsa:1369

STRING: 9606.ENSP00000359446

UniGene: Hs.2246

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