Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

Instructions
Code CSB-EL005898HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name carboxypeptidase N, polypeptide 1
Alternative Names ACBP ELISA Kit; Anaphylatoxin inactivator ELISA Kit; Arginine carboxypeptidase ELISA Kit; Carboxypeptidase N catalytic chain ELISA Kit; Carboxypeptidase N polypeptide 1 50 kD ELISA Kit; Carboxypeptidase N polypeptide 1 ELISA Kit; Carboxypeptidase N small subunit ELISA Kit; Carboxypeptidase N subunit 1 ELISA Kit; CBPN_HUMAN ELISA Kit; CPN ELISA Kit; CPN1 ELISA Kit; Kininase 1 ELISA Kit; Kininase-1 ELISA Kit; Kininase1 ELISA Kit; Lysine carboxypeptidase ELISA Kit; Plasma carboxypeptidase B ELISA Kit; SCPN ELISA Kit; Serum carboxypeptidase N ELISA Kit
Abbreviation CPN1
Uniprot No. P15169
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 mU/mL-2000 mU/mL
Sensitivity 7.8 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CPN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-95
1:2 Average % 105
Range % 101-109
1:4 Average % 87
Range % 84-92
1:8 Average % 95
Range % 91-99
Recovery
The recovery of human CPN1 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 93-103
EDTA plasma (n=4) 92 85-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
2000 1.998 2.112 2.055 1.916
1000 1.547 1.675 1.611 1.472
500 1.122 1.132 1.127 0.988
250 0.683 0.688 0.686 0.547
125 0.436 0.454 0.445 0.306
62.5 0.321 0.325 0.323 0.184
31.25 0.232 0.241 0.237 0.098
0 0.138 0.140 0.139  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Gene References into Functions
  1. CPN binds to fibrinogen and is present in a fibrin clot prepared from plasma. PMID: 23000409
  2. carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity PMID: 15718415
  3. This review summarizes the structure, enzymatic properties and function of this enzyme, including insights gained by the recent elucidation of the crystal structure of the CPN catalytic subunit and structural modeling of the non-catalytic 83 kDa subunit. PMID: 18039526
Involvement in disease Carboxypeptidase N deficiency (CPND)
Subcellular Location Secreted, extracellular space
Protein Families Peptidase M14 family
Tissue Specificity Synthesized in the liver and secreted in plasma.
Database Links

HGNC: 2312

OMIM: 212070

KEGG: hsa:1369

STRING: 9606.ENSP00000359446

UniGene: Hs.2246

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