Recombinant Human Interleukin-12 receptor subunit beta-2 (IL12RB2), partial

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Code CSB-YP859518HU
Size $250
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
IL12RB2
Uniprot No.
Research Area
Cancer
Alternative Names
I12R2_HUMAN; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2; IL12 receptor beta 2; IL12R beta2; IL12RB2; Interleukin 12 receptor beta 2; Interleukin 12 receptor beta 2 chain; Interleukin-12 receptor subunit beta-2; RP11 102M16.1
Species
Homo sapiens (Human)
Source
Yeast
Expression Region
24-622aa
Target Protein Sequence
KIDACKRGDVTVKPSHVILLGSTVNITCSLKPRQGCFHYSRRNKLILYKFDRRINFHHGHSLNSQVTGLPLGTTLFVCKLACINSDEIQICGAEIFVGVAPEQPQNLSCIQKGEQGTVACTWERGRDTHLYTEYTLQLSGPKNLTWQKQCKDIYCDYLDFGINLTPESPESNFTAKVTAVNSLGSSSSLPSTFTFLDIVRPLPPWDIRIKFQKASVSRCTLYWRDEGLVLLNRLRYRPSNSRLWNMVNVTKAKGRHDLLDLKPFTEYEFQISSKLHLYKGSWSDWSESLRAQTPEEEPTGMLDVWYMKRHIDYSRQQISLFWKNLSVSEARGKILHYQVTLQELTGGKAMTQNITGHTSWTTVIPRTGNWAVAVSAANSKGSSLPTRINIMNLCEAGLLAPRQVSANSEGMDNILVTWQPPRKDPSAVQEYVVEWRELHPGGDTQVPLNWLRSRPYNVSALISENIKSYICYEIRVYALSGDQGGCSSILGNSKHKAPLSGPHINAITEEKGSILISWNSIPVQEQMGCLLHYRIYWKERDSNSQPQLCEIPYRVSQNSHPINSLQPRVTYVLWMTALTAAGESSHGNEREFCLQGKAN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
69.7kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

N-terminally 6xHis-tagged recombinant human IL12RB2 is produced by expressing the gene fragment encoding the amino acids 24-622 of the human IL12RB2 in the yeast and fusing the 6xHis-motif to the resulting protein. Its purity is greater than 90% determined by SDS-PAGE. It migrated to the molecular weight band of about 66-90 kDa on the gel. The target protein IL12RB2 is a subunit of the IL-12 receptor complex, which is co-expressed with IL12RB1 to form the high-affinity IL-12 binding sites and to reconstitute IL-12-dependent signaling. The up-regulation of IL12RB2 is associated with numerous infectious diseases such as Crohn's disease and leprosy.

Customer Reviews and Q&A

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 Q&A
Q:

I have a customer question about item CSB-YP859518HU Recombinant human Interleukin-12 receptor subunit beta-2. I recently purchased lot # 03363.
It stated the protein is his-tagged however the amino acid of the recombinant protein shown in the page didn’t contain His tag. We also tried western blot in house and we couldn’t detect the his tag. Therefore, I want to clarity if the protein is His tagged.”
Is this protein His tagged? Do you have data to verify that the His-tag is present on Lot # 03363? If so, could you please send the testing data that shows the His tag is present?

A:
Thanks for your inquiry,We have test the protein of the same lot , and we have get the tag . Attach pls find the result.

Target Background

Function
Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Gene References into Functions
  1. The Cox proportional hazard models revealed that IL12Rb2 and p38MAPK predicted a long OS. To the best of our knowledge, the present study is the first to reveal a close association between IL12Rb2 and p38MAPK, and their possible function in nonsmall cell lung cancer progression PMID: 29956791
  2. Tumor cell differentiation is associated with TILs' expression of IL-12Rbeta2, and an IL-12Rbeta2(+) TIL ratio >/=35%) indicates favorable prognosis in LC PMID: 29103744
  3. inhibition of the H3K27 demethylase JMJD3 in naive CD4 T cells demonstrates how critically important molecules required for T cell differentiation, such as JAK2 and IL12RB2, are regulated by H3K27me3. PMID: 28947543
  4. this study identified susceptibility single nucleotide polymorphisms in IL12RB2 with Behcet's disease in Han Chinese PMID: 27464962
  5. In ankylosing spondylitis, conditional analysis identified rs11209032 as the probable causal single-nucleotide polymorphism within a 1.14 kb putative enhancer between IL23R and IL12RB2. The rs11209032 single-nucleotide polymorphism downstream of IL23R forms part of an enhancer, allelic variation of which may influence Th1-cell numbers. PMID: 26916345
  6. study confirmed the relationship of IL12RB2 polymorphisms with primary biliary cholangitis (PBC) susceptibility; provided evidence that IL12RB2 polymorphisms are associated with liver cirrhosis and an increased concentration of disease-specific anti-mitochondrial antibodies in sera of PBC patients PMID: 28299343
  7. this study shows that single nucleotide polymorphisms of the IL23R-IL12RB2 region are associated with Behcet's disease in a Northern Chinese Han population PMID: 27660093
  8. IL12RB2 polymorphisms correlate with risk of lung adenocarcinoma. PMID: 26547104
  9. The effects of rs3762315 and rs3762316 single nucleotide polymorphisms in 5' flanking region on IL12RB2 transcription PMID: 26552659
  10. Estrogen receptor beta 2 regulates IL12RB2 expression via p38 MAPK signaling and inhibits non-small-cell lung cancer proliferation and invasion. PMID: 25695486
  11. IL12RB2 polymorphism is associated with systemic lupus erythematosus in the Chinese population. PMID: 25720506
  12. results suggest the rs924080 risk A allele does not affect baseline expression of IL-12RB2, IL-23R, IFN-gamma and TNF-alpha but enhances IL-23R and TNF-alpha expression capacity in response to LPS stimulation; the A allele appears to enhance baseline IL-6 production PMID: 24434271
  13. a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero PMID: 22715389
  14. Polymorphisms present in the promoter region of IL12RB2 may not be associated with susceptibility to leprosy or its clinical forms. PMID: 24486579
  15. ERbeta2 and IL-12Rbeta5 had longer OS. PMID: 23677568
  16. Data indicate that four of the IL-12Rbeta2 variants, N271Y, R313G, A604V and L808R showed reduced IL-12 responses compared to the wild type variant. PMID: 23911393
  17. Association of IL12RB2 rs6679356 polymorphism with the age of type 1 diabetes mellitus onset suggests that this gene plays a role in defining the time of disease onset. PMID: 23152861
  18. the -237 polymorphic site in the 5' promoter region of the IL-12Rbeta2 (SNP ID: rs11810249) gene associated with the AP-4 transcription motif GAGCTG PMID: 22509293
  19. data clearly support the IL12RB2 genetic association with systemic sclerosis (SSc), and suggest a relevant role of the interleukin 12 signaling pathway in SSc pathogenesis. PMID: 22076442
  20. Our results support a potential influence of the rs3790567 IL12RB2 polymorphism in the pathogenesis of giant cell arteritis. PMID: 21285166
  21. significant interaction between the IL-12RB1 and IL-12RB2 genes contributes to a 4-fold increased risk for developing extrinsic atopic dermatitis PMID: 20060272
  22. STATE OF ART REVIEW. IL-12Rb2 gene is not expressed in EBV-transformed normal B-lymphocytes and in Burkitt's lymphoma B-cell lines. PMID: 11940489
  23. upregulated in Il-12 augmented T cell activation of mononclear leukocytes from HIV seropositive individuals PMID: 12804004
  24. promoter activity is increased in case of the -465G allele, disrupting the intact GATA site PMID: 15140029
  25. Study showed recombinant human interleukin-12 (rhIL-12) safe/well tolerated in healthy subjects given single doses (1 up to 8 ug) subcutaneously. Interferon-gamma and, signal transducer and activator of transcription may be useful biomarkers of rhIL-12. PMID: 15901746
  26. Epigenetic silencing of IL-12Rbeta2 is a frequent event in lung cancers. Aberrant methylation of this gene seems to be a useful predictor of long-term outcome for adenocarcinoma of the lung. PMID: 17602269
  27. frequencies of variant alleles were significantly higher in aggressive periodontitis patients as compared with healthy controls or chronic periodontitis patients PMID: 18353079
  28. IL-12R beta 2 directly restrains multiple myeloma cell growth PMID: 18474725
  29. These results suggest that these SNPs in IL12RB2 have differential effects on cellular activation of T cells and NK cells. PMID: 18771340
  30. Single Nucleotide Polymorphism in interleukin 12 receptor beta 2 is associated with susceptibility to radiation dermatitis in breast cancer. PMID: 18927311
  31. Our data show significant associations between primary biliary cirrhosis and common genetic variants at the HLA class II, IL12A, and IL12RB2 loci PMID: 19458352
  32. Studies established the concept that the IL-12Rbeta2 gene is a gatekeeper from cancer. PMID: 19720917
  33. In the generation of cell-mediated immunity against intracellular infection, the expression and function of the IL-12 receptor beta 2 chain correlate with the response of Th1-type cells to Mycobacterium leprae antigen. PMID: 11441083

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Type I cytokine receptor family, Type 2 subfamily
Tissue Specificity
Isoform 2 is expressed at similar levels in both naive and activated T-cells.
Database Links

HGNC: 5972

OMIM: 601642

KEGG: hsa:3595

STRING: 9606.ENSP00000262345

UniGene: Hs.479347

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