Code | CSB-YP018603HU |
Abbreviation | Recombinant Human PRAME protein |
MSDS | |
Size | $250 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
To achieve the expression of the recombinant Human PRAME protein in Yeast cells, a DNA fragment encoding the Human PRAME protein (1-509aa) is inserted into a plasmid vector, which is then transferred to Yeast cells. After screening positive cells, they are cultured and induced to generate the PRAME protein. A N-terminal 6xHis tag is attached to the protein. Cells are lysed to collect the recombinant Human PRAME protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the obtained recombinant Human PRAME protein is over 90%.
There are currently no reviews for this product.