PRAME Antibody

Datasheet
Code CSB-PA018603LA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: A375 whole cell lysate, Mouse brain tissue
    All lanes: PRAME antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA018603LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA018603LA01HU at dilution of 1:100

  • Immunofluorescence staining of HepG2 cells with CSB-PA018603LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PRAME Polyclonal antibody
Uniprot No. P78395
Target Names PRAME
Alternative Names Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma), PRAME, MAPE OIP4
Raised in Rabbit
Species Reactivity Human, Mouse
Immunogen Recombinant Human Melanoma antigen preferentially expressed in tumors protein (1-509AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
Subcellular Location Nucleus, Cell membrane
Protein Families PRAME family
Tissue Specificity Expressed in testis. Detected in samples of kidney, brain and skin.
Database Links

HGNC: 9336

OMIM: 606021

KEGG: hsa:23532

STRING: 9606.ENSP00000381726

UniGene: Hs.30743

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