Recombinant Human Methylcytosine dioxygenase TET3 (TET3), partial

Code CSB-YP023398HU
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Source Yeast
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Code CSB-EP023398HU
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Source E.coli
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Code CSB-EP023398HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023398HU
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Source Baculovirus
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Code CSB-MP023398HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TET3
Uniprot No.
Alternative Names
hCG_40738; KIAA0401; Methylcytosine dioxygenase TET3; Probable methylcytosine dioxygenase TET3; Tet methylcytosine dioxygenase 3; Tet oncogene family member 3; Tet3; TET3_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.
Gene References into Functions
  1. High mRNA levels of TET3 is independent predictor of poor outcome in Renal Cell Carcinoma patients. PMID: 28069330
  2. TET3 interacts with different members of the thyroid hormone nuclear receptors superfamily and also enhances their association to chromatin. PMID: 28716910
  3. TET3-5-hydroxymethylcytosine was upregulated in the femoral head tissues of SAON patients and MLO-Y4 cells with dexamethasone (Dex) treatment. PMID: 27627619
  4. Results revealed that TET3 acted as a suppressor of ovarian cancer by demethylating miR-30d precursor gene promoter to block TGF-beta1-induced epithelial-mesenchymal transition. PMID: 27141829
  5. TET3 expression inhibits glioblastoma tumorigenesis and self-renewal in glioblastoma stem cells. PMID: 26838672
  6. levels of TET3 and TDG mRNAs were independent prognostic factors for early breast cancer patients who received anthracycline chemotherapy PMID: 26207381
  7. Hypoxia deregulates TET3. TET1/3 levels were associated with tumor hypoxia, tumor malignancy, and poor prognosis in breast cancer patients. Coordinate functions of TET1 and TET3 were needed to activate TNFalpha-p38-MAPK signaling in hypoxia. PMID: 26294212
  8. In hepatic stellate cells, cell proliferation rise significantly and cell apoptosis reduce obviously after knockdown of TET3. PMID: 25550811
  9. OGT catalyzes the O-GlcNAcylation of TET3, promotes TET3 nuclear export, and, consequently, inhibits the formation of 5-hydroxymethylcytosine catalyzed by TET3. PMID: 24394411
  10. TET3 enhanced its localization to chromatin through the stabilization of OGT protein. PMID: 24304661
  11. variable association with CXXC modules may contribute to context specific functions of Tet proteins PMID: 23690950
  12. Studies indicate that the TET-family enzymes are Fe(II), 2-oxoglutarate-dependent oxygenases and catalyze demethylation of 5-methylcytosine (5mC) by iterative oxidation reactions. PMID: 23727577

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Subcellular Location
Nucleus. Cytoplasm. Chromosome.
Protein Families
TET family
Tissue Specificity
Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
Database Links

HGNC: 28313

OMIM: 613555

KEGG: hsa:200424

STRING: 9606.ENSP00000386869

UniGene: Hs.516107

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