Recombinant Human Ras-related protein Rab-11A(RAB11A)

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Code CSB-EP019153HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names RAB11A
Uniprot No. P62491
Research Area Cancer
Alternative Names MGC1490; Rab 11; Rab 11A; RAB 11A, member oncogene family; Rab-11; RAB11 A; RAB11; Rab11a; RAB11A member RAS oncogene family; Ras related protein Rab 11A; Ras related protein Rab11A; Ras-related protein Rab-11A; RB11A_HUMAN; YL 8; YL8
Species Homo sapiens (Human)
Source E.coli
Expression Region 2-213aa
Target Protein Sequence GTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 39.9kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
Gene References into Functions
  1. irregularly coated vesicle formation is observed only in influenza A virus infected cells and requires an active Rab11 GTPase. PMID: 29123131
  2. Study suggestes that Rab11a serves as an important oncoprotein and a regulator of YAP in human non-small cell lung cancer. PMID: 28468127
  3. This review will focus on the structure and function of the novel Rab11-FIP3-Rabin8 dual effector complex and its implications for the targeting of sensory receptors to primary cilia, dysfunction of which causes cilia defects underlying human diseases and disorders know as ciliopathies. PMID: 26399276
  4. Data suggest that membrane tethering mediated by endosomal RAB11A is drastically and selectively stimulated by its cognate Rab effectors, class V myosins (MYO5A and MYO5B), in a GTP-dependent manner. (RAB11A = ras-related GTPase Rab-11A; MYO5 = myosin class V) PMID: 28939769
  5. these studies are not only the first to identify alpha4 helix of Rab11a as a protein binding domain on the GTPase but also reveal novel mechanistic insights into the intracellular trafficking of the hIP, and potentially of other members of the GPCR superfamily, involving Rab11-dependent mechanisms PMID: 28739266
  6. transient expression of ATG16L1 not only inhibited autophagosome biogenesis, but also aberrantly targeted RAB11-positive recycling endosomes, resulting in recycling endosome aggregates. PMID: 27875067
  7. The results from this analysis indicated that Rab11a, Rab11c(Rab25) and Rab14 were expressed in a wide range of cell lines, including the human placental trophoblastic BeWo cell line. PMID: 28922401
  8. The results suggest that progressive assembly of vRNA segments may be cell type dependent and that vRNA may be transported through the cytoplasm without Rab11A-containing recycling endosomes in the absence of intact microtubules. PMID: 28724771
  9. Rac1-Rab11-FIP3 regulatory hub coordinates vesicle traffic with actin remodeling and T-cell activation PMID: 27154205
  10. In colorectal carcinoma, overexpression of Rab11 was found to promote collective cell migration through the increased distribution of E-cadherin, which enhanced cell-cell connections. PMID: 27696383
  11. A novel tribasic Golgi export signal directs cargo protein interaction with activated Rab11 and AP-1-dependent Golgi-plasma membrane trafficking. PMID: 26941330
  12. Analysis reveals novel aspects of the PI4KIIIb-Rab11 complex, specifically conformational changes induced in the switch regions of activated Rab11. PMID: 26756197
  13. Substituted V20 of Rab11A was found to interfere with proper localization of the water molecule, which mediated GTP hydrolysis, resulting in GTP being locked in an active form of Rab11A S20V. PMID: 26879265
  14. Data (including data from studies in knockout mice) suggest Munc13-4 binds to Rab11 and regulates trafficking of Rab11-containing vesicles; Munc13-4 appears to regulate final steps of Rab11-positive vesicle docking at plasma membrane in exocytosis. PMID: 26637356
  15. Rab11A and Rab11B differentially regulate intracellular trafficking of PAR1 through distinct endosomal sorting mechanisms PMID: 26635365
  16. Rab11a/Rab11 family-interacting protein 2-mediated VE-cadherin recycling is required for formation of adherens junctions and restoration of vascular endothelial barrier integrity. PMID: 26663395
  17. Mumps Virus release from the apical surface of polarized epithelial cells is facilitated by a Rab11-mediated transport system. PMID: 26378159
  18. Vaccinia virus traffics to early endosomes, where recycling endosome markers Rab11 and Rab22 are recruited to participate in subsequent virus trafficking prior to virus core uncoating in the cytoplasm. PMID: 26041286
  19. Rab11a-knockdown cells displayed mislocalization of STX3 and the presentation of microvilli in the basolateral compartment PMID: 25673875
  20. Rab11 co-localizes with alpha-synuclein in intracellular inclusions and modulates its aggregation, secretion and toxicity. PMID: 25092884
  21. PI4KIIIbeta likely plays a role in breast oncogenesis and that cooperation between Rab11a and PI4KIIIbeta represents a novel Akt activation pathway. PMID: 24962317
  22. Additionally, Rab11 recruitment is absent and Shigella-containing vacuolar rupture is delayed in the ipgD mutant that does not dephosphorylate PI(4,5)P2 into PI(5)P. PMID: 25299335
  23. Data demonstrated that Rab11 regulated E-cadherin expression and promoted colon cancer cell transformation. PMID: 25117932
  24. Rab11-dependent EGFR recycling has a role in synergistic cytotoxicity of afatinib and cetuximab against EGFR T790M PMID: 25446083
  25. Microvilli establishment required interaction between RAB8A and MYO5B, while loss of the interaction between RAB11A and MYO5B induced microvillus inclusions. PMID: 24892806
  26. Ubiquitylation and activation of RAB11a is promoted by a beta2 adrenergic receptor-HACE1 complex. PMID: 24190883
  27. Rab11A controls BACE1 recycling to endosomes and thus affects beta-amyloid production. PMID: 24373285
  28. these data provide a mechanism for how PI4KIIIbeta coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIbeta to combat malaria. PMID: 24876499
  29. Mitochondrial remodeling was associated with increased proximity between Rab11a and mitochondrial membranes, changes in fusion-fission dynamics, and mitochondrial relocalization of the Drp1, which was regulated by the Rab11a effector protein FIP1/RCP. PMID: 24302731
  30. Data indicate that alpha2-macroglobulin (alpha2M*), through its receptor LRP1, induces cellular migration of Muller glial cells by a mechanism that involves MT1-MMP intracellular traffic to the plasma membrane by a Rab11-dependent recycling pathway. PMID: 23640058
  31. Dominant negative (GDP-locked)-Rab5 and -Rab11 reproduced the effects of inhibitors of tubulin and actin, respectively, on the cycling of bradykinin B receptor. PMID: 23454239
  32. These findings suggest that Rab11-FIP1 proteins participate in spatially and temporally distinct steps of the recycling process along a complex and dynamic tubular network in which Rab11-FIPs occupy discrete domains. PMID: 23283983
  33. The potential roles of the Rab11 pathway in the trafficking, assembly and budding steps of negative-strand viruses. PMID: 23176490
  34. Rab11 depletion gives rise to accumulation of recycling carriers containing endocytosed transferrin and transferrin receptor beneath the plasma membrane. PMID: 22685325
  35. findings show 2 mother centriole appendage proteins, centriolin and cenexin/ODF2, regulate association of the endosome components Rab11, the Evi5, and the exocyst at the mother centriole PMID: 22981775
  36. Rab11A/myosin Vb/Rab11-FIP2 complex frames two late recycling steps of langerin from the ERC to the plasma membrane PMID: 22420646
  37. TBC1D14 binds activated Rab11 but is not a GAP for Rab11, and loss of Rab11 prevents TBC1D14-induced tubulation of recycling endosomes. PMID: 22613832
  38. Our data suggest a selective role of PICK1 in clustering and reducing the recycling rates of PDZ domain binding partners sorted to the Rab11-dependent recycling pathway. PMID: 22303009
  39. Recycling was accompanied by dephosphorylation of VEGFR-2 between Rab4 and Rab11 vesicles and quantitatively and qualitatively altered signal output. PMID: 21586748
  40. Our results uncover a critical host factor with an essential contribution to influenza virus genome delivery and reveal a potential role for RAB11A in the transport of ribonucleoprotein cargo. PMID: 21525351
  41. The authors propose that influenza virus ribonucleoproteins are routed from the nucleus to the pericentriolar recycling endosome (RE), where they access a Rab11-dependent vesicular transport pathway to the cell periphery. PMID: 21307188
  42. Rabin8 and Rab11 are associated with the Bardet-Biedl syndrome. PMID: 21273506
  43. Rab11a, but not Rab8a, is required for recycling of transferrin in nonpolarized cells. PMID: 21282656
  44. Rab11a played a prominent role in recycling of the wild type ss1-adrenergic receptors because dominant negative rab11a inhibited. PMID: 20727405
  45. Rab11a is an important regulator of TLR4 and Toll-receptor-associated molecule transport to E. coli phagosomes thereby controlling IRF3 activation from this compartment. PMID: 20933442
  46. Mutant prion protein expression is associated with an alteration of the Rab GDP dissociation inhibitor alpha (GDI)/Rab11 pathway. PMID: 19996123
  47. Results describe the involvement of two Rab GTPases, Rab9 and Rab11, in the trafficking of TRPC6. PMID: 20346379
  48. Transmission electron microscopy of thin sections confirmed that Rab11 depletion resulted in significant numbers of abnormally formed virus particles that had failed to pinch off from the plasma membrane. PMID: 20357086
  49. Rab11, in its GTP-bound form, interacts with Rabin8 and kinetically stimulates the guanine nucleotide-exchange activity of Rabin8 toward Rab8. PMID: 20308558
  50. mutation impairs a specific subset of Rab11 interactions, possibly those involved in cytoskeleton-based movements driving the slow recycling pathway PMID: 14699104
  51. Rab11a, RPL7, and RPL28 showed moderate levels of differential expression in esophageal squamous cell carcinoma PMID: 15188492
  52. there is a pivotal role for rab11 in regulating the traffic of a G protein-coupled receptor at the level of the recycling endosome PMID: 15190120
  53. an interaction between Rab11 and TPbeta directs TPbeta recycling PMID: 16126723
  54. Data suggest that polarization of hepatocytes requires recruitment of rab11a and myosin Vb to intracellular membranes that contain apical ABC transporters and transcytotic markers. PMID: 16214890
  55. We conclude that the glutamine residue of Rab11a at position 70 is not strictly essential for GTPase activity of this protein in contrast to Ras and other Rab proteins. PMID: 16473307
  56. Results describe the distinct C-terminal binding regions of FIP3 and FIP4 for Rab11 and ARF5/ARF6, and the crystal structure of Rab11 in complex with the Rab11-binding domain (RBD) of FIP3. PMID: 17030804
  57. Rab 11 and Arf6 function synergistically in the switch from furrowing to abscission, as well as in the terminal stage of abscission PMID: 17157409
  58. CaR is constitutively endocytosed and recycled to the plasma membrane by a Rab11a-dependent mechanism. PMID: 17426287
  59. myosin Vb is required for CFTR recycling in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells PMID: 17462998
  60. Rab11 plays a role in the intracellular trafficking of various types of LG-molecule from the TGN to the cell surface PMID: 17476295
  61. These results show that Rab11A and Langerin are required for BG biogenesis, and they illustrate the role played by a Rab GTPase in the formation of a specialized subcompartment within the endocytic-recycling system. PMID: 17538027
  62. These data therefore show that (i) Rab11a regulates cell surface abundance of both GLUT4 and FAT/CD36. PMID: 17854769
  63. Both phosphorylated and nonphosphorylated MOR internalize via Rab5-dependent pathway after agonist stimulation, and the phosphorylated and nonphosphorylated MORs recycle through distinct vesicular trafficking pathways mediated by Rab4 and Rab11. PMID: 18550774
  64. endosomal activation of the PI3K/AKT signaling pathway in response to Gbetagamma trafficking, via its interaction with Rab11, is a relevant step in the mechanism controlling cell survival and proliferation PMID: 18701709
  65. Rab11a was expressed in umbrella cells (but not Rab11b or Rab25) and was associated with discoidal/fusiform vesicles, which were dependent on the activity of the Rab11a GTPase for exocytosis. PMID: 18843107
  66. Both rab11a and HSP90 appear to be involved in the process of exocytosis of internalized extracellular alpha-synuclein. PMID: 19193894
  67. Recycling endosome trafficking to the Golgi complex through a pathway involving Src-family kinases, Cdc42, and Rab11a plays a general role in death signaling by mediating regulated changes in Golgi dynamics. PMID: 19641023
  68. A novel mechanism for cellular dysfunction by the HD mutation arising from the inhibition of Rab11 activity and a deficit in vesicle formation at recycling endosomes, is proposed. PMID: 19752198
  69. D-AKAP2 promotes accumulation of recycling proteins in the Rab4/Rab11-positive endocytic recycling compartment PMID: 19797056

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Subcellular Location Cell membrane, Lipid-anchor, Recycling endosome membrane, Lipid-anchor, Cleavage furrow, Cytoplasmic vesicle, phagosome
Protein Families Small GTPase superfamily, Rab family
Database Links

HGNC: 9760

OMIM: 605570

KEGG: hsa:8766

STRING: 9606.ENSP00000261890

UniGene: Hs.321541

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