Recombinant Human Sulfotransferase family cytosolic 2B member 1(SULT2B1)

Code CSB-EP022943HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names SULT2B1
Uniprot No. O00204
Research Area Cardiovascular
Alternative Names Alcohol sulfotransferase; EC 2.8.2.2; HSST2; Hydroxysteroid sulfotransferase 2; ST2B1; ST2B1_HUMAN; Sulfotransferase 2B1; Sulfotransferase family cytosolic 2B member 1; SULT2B1; SULT2B1a; SULT2B1b
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-365aa
Target Protein Sequence MDGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDEDPEEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 68.3kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Plays a role in epidermal cholesterol metabolism and in the regulation of epidermal proliferation and differentiation
Gene References into Functions
  1. findings showed clearly the impact of genetic polymorphisms on the cholesterol-sulphating activity of SULT2B1b allozymes, which may underscore the differential metabolism of cholesterol in individuals with different SULT2B1b genotypes PMID: 29701841
  2. Mutation in SULT2B1 leads to an autosomal-recessive congenital ichthyosis phenotype via increased proliferation of human keratinocytes, thickening of epithelial layers, and altered epidermal cholesterol metabolism. PMID: 28575648
  3. Since 1'-hydroxyestragole exposure readily produced DNA injury in B-13/H cells, these data suggest that cholangiocarcinomas generated in rats fed estragole may be dependent, in part, on SULT2B1 activation of the 1'-hydroxyestragole metabolite. PMID: 26739637
  4. Overexpression of SULT2B1b is an independent prognostic indicator and promotes cell growth and invasion in colorectal carcinoma. PMID: 26121319
  5. This study identified specific germline variations in estradiol metabolism-related pathways, namely CYP1B1, SULT2B1, and HSD17B2, as novel prognostic markers that are cumulatively associated with increased risk of prostate cancer progression PMID: 24682418
  6. The SULT2B1 isoforms have a unique 50 amino acid carboxy-terminal sequence that is not present in the other human SULT isoforms (review). PMID: 24020383
  7. Data indicate that cholesterol sulfate (CS) and SULT2B1b inhibited gluconeogenesis by targeting the gluconeogenic factor hepatocyte nuclear factor 4alpha (HNF4alpha) in both cell cultures and human SULT2B1b transgenic mice. PMID: 24277929
  8. SULT2B1 is induced by calcitriol activated vitamin D receptor in human prostate cancer cells. PMID: 23579488
  9. SULT2B1b expression promotes proliferation of hepatocellular carcinoma cells in vitro and in vivo, which may contribute to the progression of HCC. PMID: 23593328
  10. In HT29 cells expression of SULT2B1 was enhanced by probiotic fermentation supernatants without aleurone. PMID: 21840698
  11. The Ca(2+)-bound form may be physiologically relevant for stressed cells with an elevated free calcium level. PMID: 20329768
  12. The expression of SULT2B1b mRNA in endometrial stromal cells is induced by progesterone and that in endometrial epithelial cells is induced by relaxin via the cAMP pathway. PMID: 19243756
  13. alternative splicing of SULT2B1 results in cholesterol sulfotransferase or pregnenolone sulfotransferase PMID: 12145317
  14. crystal structure of hydroxysteroid sulfotransferases SULT2B1a and SULT2B1b bound to the substrate donor product 3'-phosphoadenosine 5'-phosphate and acceptor substrate pregnenolone revealed a different catalytic binding orientation PMID: 12923182
  15. We have documented the presence of the enzyme (SULT2B1b) that sulfonates cholesterol in human platelets and examined the influence of plasma lipoproteins on the expression and activity of this enzyme PMID: 14676143
  16. Differential subcellular localization of SULT2B1b in prostate and placenta suggests that SULT2B1b may be differentially regulated and have different physiological functions in these two hormonally responsive human tissues PMID: 14741047
  17. confinement of SULT2B1b to the granular layer coincides with this being the area with the highest cholesterol sulfate content suggesting that the physiologic action of cholesterol sulfate is likely carried out in this region of the living epidermis PMID: 15140224
  18. Immunohistochemical localization of SULT2B1b in human tissues showed expression in the cytoplasm of ciliated columnar or cuboidal epithelial cells in terminal bronchia. PMID: 15878639
  19. Of five Sp1 elements identified in SULT2B1, two had regulatory activity utilizing immortalized human keratinocytes. PMID: 15953604
  20. SULT2B1b, by acting on a variety of oxysterols, offers a potential pathway for modulating in vivo the injurious effects of these compounds. PMID: 17347498
  21. Results show SULT2B1 polymorpohisms affect enzyme functions. PMID: 17496163
  22. SULT2B1 is involved in reulating prostate cell responsiveness to dehydroepiandrosterone and delta5-androstenediol. PMID: 17626250
  23. Data show that SULT2B1b is localized in cytosol and nuclei of cancerous and normal breast tissue, and that African Americans had significantly more SULT2B1b in nuclei of epithelial cells of normal breast tissue compared to Caucasians. PMID: 18586095

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Involvement in disease Ichthyosis, congenital, autosomal recessive 14 (ARCI14)
Subcellular Location Cytoplasm, Microsome, Nucleus, Cytoplasm, cytosol
Protein Families Sulfotransferase 1 family
Tissue Specificity Expressed in the stratum granulosum-stratum corneum junction in the skin (at protein level). Expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung.
Database Links

HGNC: 11459

OMIM: 604125

KEGG: hsa:6820

STRING: 9606.ENSP00000201586

UniGene: Hs.369331

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