Recombinant Human T-box transcription factor TBX21(TBX21)

Code CSB-YP890744HU
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Source Yeast
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Code CSB-EP890744HU
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Source E.coli
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Code CSB-EP890744HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890744HU
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Source Baculovirus
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Code CSB-MP890744HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names TBX21
Uniprot No. Q9UL17
Alternative Names T bet; T box 21; T box expressed in T cells; T box protein 21; T box transcription factor TBX21; T cell specific T box transcription factor; T cell specific T box transcription factor T bet; T PET; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; TBET; TBLYM; TBX 21; Tbx21; TBX21_HUMAN; TPET; Transcription factor TBLYM
Species Homo sapiens (Human)
Expression Region 1-535
Target Protein Sequence MGIVEPGCGD MLTGTEPMPG SDEGRAPGAD PQHRYFYPEP GAQDADERRG GGSLGSPYPG GALVPAPPSR FLGAYAYPPR PQAAGFPGAG ESFPPPADAE GYQPGEGYAA PDPRAGLYPG PREDYALPAG LEVSGKLRVA LNNHLLWSKF NQHQTEMIIT KQGRRMFPFL SFTVAGLEPT SHYRMFVDVV LVDQHHWRYQ SGKWVQCGKA EGSMPGNRLY VHPDSPNTGA HWMRQEVSFG KLKLTNNKGA SNNVTQMIVL QSLHKYQPRL HIVEVNDGEP EAACNASNTH IFTFQETQFI AVTAYQNAEI TQLKIDNNPF AKGFRENFES MYTSVDTSIP SPPGPNCQFL GGDHYSPLLP NQYPVPSRFY PDLPGQAKDV VPQAYWLGAP RDHSYEAEFR AVSMKPAFLP SAPGPTMSYY RGQEVLAPGA GWPVAPQYPP KMGPASWFRP MRTLPMEPGP GGSEGRGPED QGPPLVWTEI APIRPESSDS GLGEGDSKRR RVSPYPSSGD SSSPAGAPSP FDKEAEGQFY NYFPN
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation. Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells. Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program.
Gene References into Functions
  1. The repression of TBX21 expression by high-affinity binding of YY1 to the -1993C allele may contribute to a decreased development of AIH-1 via suppression of type 1 immunity. PMID: 29358858
  2. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  3. siRNA-mediated knockdown of T-bet and RORgammaT contributes to decreased inflammation in preeclampsia. PMID: 28849203
  4. Investigated the associations of IL-28B rs12979860 and TBX21 rs17250932, rs4794067 polymorphisms with the susceptibility to, and outcomes of, hepatitis C virus (HCV) infection; rs4794067 variant genotypes significantly correlated with increased risk of HCV chronic infection and susceptibility PMID: 29399747
  5. TBX21 expression level showed a reduction expression in aged men and women. PMID: 28509479
  6. Studies indicate the accumulation of T-bet transcription factor (T-bet)+ B cells in autoimmune patients. PMID: 28641866
  7. Data show that T-box transcription factor TBX21 (T-bet) expressed in unstimulated memory more than naive B cells, and more in young individuals. PMID: 28457482
  8. Studies indicate the induction of T-bet transcription factor (T-bet) in B cells following both HIV and HCV infections. PMID: 28739077
  9. Studies indicate that CD11c+ T-bet+ memory B cells exhibit a distinct transcriptome. PMID: 28838763
  10. Studies indicate a feedback loop amongst T-bet, STAT and cytokines. PMID: 28923237
  11. Studies indicate enriched expression of TBX21 (T-bet), which is important for B cell survival and response to antigen, was observed in CD11c+ B cells. PMID: 28756897
  12. the model of mutually antagonistic differentiation programs driven by mutually exclusively expressed T-bet or GATA-3 does not completely explain natural CD4 T cell priming outcomes PMID: 29088218
  13. The aim of this study was to investigate the clinical significance of three immune cell-related transcription factors, T-bet, GATA-3 and Bcl-6 in bladder cancer in Tunisian patients. PMID: 27237631
  14. this study shows that T-bet restrains IFN-gamma-induced collateral type I IFN circuitry in the Th1 response in vivo PMID: 28623086
  15. this review discusses how T-bet regulates transcriptional programs in response to type 1 inflammatory signals PMID: 28279590
  16. HDAC11-knockout T cells displayed enhanced proliferation, proinflammatory cytokine production, and effector molecule expression of Eomes and TBX21 transcription factors previously shown to regulate inflammatory cytokine and effector molecule production. PMID: 28550044
  17. severe but not euthyroid Hashimoto's thyroiditis is associated with robust upregulation of T-bet and FOXP3 mRNA in peripheral T cells, independent of the thyroid hormone status but proportional to disease activity PMID: 27478306
  18. T-helper Cell Type-1 Transcription Factor T-Bet Is Down-regulated in Type 1 Diabetes. PMID: 27917625
  19. Results suggest that genetic polymorphisms may predispose individuals to mucosal autoimmune disease through alterations in transcription factor T-bet (TBX21) binding. PMID: 28187197
  20. There is a genetic association of ankylosing spondylitis with TBX21 which influences T-bet and pro-inflammatory cytokine expression in humans and SKG mice as a model of spondyloarthritis. PMID: 27125523
  21. this study shows that in peripheral blood, end-stage renal disease patients show increased levels of t-bet PMID: 26970513
  22. IFNG and IFNG-TBX21 allele interaction are involved in systemic lupus erythematosus susceptibility in a Chinese Han population. PMID: 26916970
  23. expression of T-bet may influence the function of HBV-specific CD8+ T cells and thus can be an attractive target for modulation to improve HBV-specific immunity in CHB. PMID: 26809262
  24. HMGB1 contributed to IFN-gamma-producing Th17-cell bias in coronary artery atherosclerosis by controlling expression of T-bet and RUNX3. PMID: 26520896
  25. A high copy number of T-bet and GATA-3 confers susceptibility to AAU and AS, and a high copy number of FOXP3 confers susceptibility to female patients with AAU either with or without AS. PMID: 27082299
  26. There is a common program of effector T cell differentiation that is regulated by IL-2 and IL-12 signaling and the combined activities of the transcriptional regulators Blimp-1 and T-bet. PMID: 26950239
  27. TBX21 expression was low in multiple sclerosis (MS), and stable over time. The low EOMES/TBX21 phenotype in MS reflects cd56+ cell dysregulation. PMID: 26762769
  28. Data indicate that overexpression of micrRNA miR-135a alters transcription factor T-bet and GATA binding protein 3 (GATA-3) mRNA expression in allergic rhinitis (AR). PMID: 26418311
  29. Our data indicate that Tim-3 expression on NK cells is regulated by T-bet, and that Tim-3 levels correlate with advanced stages of gastric cancer PMID: 26214042
  30. T-bet expression might be associated with differentiation into effector memory cells and PD-1 expression in mycobacterial antigen-specific CD4(+) T cells. PMID: 26302932
  31. Our results suggested that TBX21 variants may be involved in the etiology of this disease. PMID: 25759111
  32. the level of T-bet and Eomesodermin, two T-box transcription factors regulating lymphocyte effector functions, is strongly reduced in NK cells from allogeneic hematopoietic stem cell transplantation recipients compared with healthy control subjects. PMID: 26438526
  33. observed the polymorphic allele T was enriched in the chronic periodontitis patients compared to chronic gingivitis patients; investigating the putative functionality TBX21-1993T/C in modulation of local response, observed transcript levels of T-bet, but not of IFN-gamma, were upregulated in homozygote and heterozygote polymorphic subjects PMID: 25832120
  34. The tested 25 SNPs in TBX21, GATA3, Rorc and Foxp3 did not associate with BD and VKH syndrome. PMID: 25873156
  35. Data show that the expression levels of transcription factors GATA-3 and FOXP3 were upregulated with 1.0 mug/ml galectin-1, while transcription factors TBX21 and RORC expression levels were reduced with both 1.0 and 2.0 mug/ml concentrations of galectin-1. PMID: 25292313
  36. Studies indicate that a key feature of age-associated B cells (ABCs) is that they express and depend upon B cell-intrinsic expression of the T-bet transcription factor. PMID: 26297793
  37. study concludes that TBX21 gene polymorphism was associated with an increased risk of asthma in Indian population PMID: 25056814
  38. The mRNA level of Th1-specific transcription factor T-bet was reduced in patients with liver cancer. PMID: 25552913
  39. This study supports the concept that poor human viral-specific CD8(+) T cell functionality is due to an inverse expression balance between T-bet and Eomes PMID: 25032686
  40. Polymorphism rs9910408 in TBX21 gene was associated with response to inhaled corticosteroids in asthma. PMID: 24107858
  41. Expression of T-bet was high in gammadeltaT cells that were expanded in the Th2 polarizing condition. PMID: 25575062
  42. Especially, the ACC, ACT, and ATC haplotypes derived from the TBX21 gene would increase the susceptibility to ESCC in the high-risk Chinese population. PMID: 25577251
  43. High tumor nuclei T-bet expression in primary tumors of breast cancer was correlated with poor prognosis and high density of T-bet+ interstitial lymphocytes in primary tumors of breast cancer were correlated with favorable prognosis. PMID: 25400774
  44. Knockdown of T-bet expression in Mart-127-35 -specific T-cell-receptor-engineered human CD4(+) CD25(-) and CD8(+) T cells attenuates effector function. PMID: 25495780
  45. The transcription factor, t-bet, primes intestinal transplant rejection, and associates with disrupted homeostatic relationships between innate and adaptive immune cells in the allograft mucosa during rejection. PMID: 25340608
  46. Results indicate that the pre-existence and nuclear mobilization of transcription factor T-bet in resting memory CD4(+) T cells might be a possible transcriptional mechanism for rapid production of cytokines by memory CD4(+) T cells. PMID: 25378399
  47. expression in CD8+ T cells during early chronic infection differentiates controllers from relapsers PMID: 25339676
  48. Chronic lymphocytic leukemia cells express CD38 in response to Th1 cell-derived IFN-gamma by a T-bet-dependent mechanism. PMID: 25505279
  49. TOX2 plays a crucial role in controlling normal NK cell development by acting upstream of TBX21 transcriptional regulation PMID: 25352127
  50. In the presence of 100 ng/ml IL-11, GATA-3 transcript abundance rose up to ~85-fold of that measured in untreated cells, whereas T-bet transcripts were lowered merely to ~41% PMID: 24338248

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Involvement in disease Asthma, with nasal polyps and aspirin intolerance (ANPAI)
Subcellular Location Nucleus.
Tissue Specificity T-cell specific.
Database Links

HGNC: 11599

OMIM: 208550

KEGG: hsa:30009

STRING: 9606.ENSP00000177694

UniGene: Hs.272409

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