Recombinant Human Ubiquitin carboxyl-terminal hydrolase CYLD(CYLD) ,partial

Code CSB-YP865105HU
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Source Yeast
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Code CSB-EP865105HU
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Source E.coli
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Code CSB-EP865105HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865105HU
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Source Baculovirus
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Code CSB-MP865105HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CYLD
Uniprot No. Q9NQC7
Alternative Names BRSS; CDMT; Cyld; CYLD gene; CYLD_HUMAN; CYLD1; CYLDI; cylindromatosis (turban tumor syndrome); cylindromatosis 1; Deubiquitinating enzyme CYLD; EAC; HSPC057 ; KIAA0849; MFT; MFT1; Probable ubiquitin carboxyl terminal hydrolase CYLD; SBS; TEM; turban tumor syndrome; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; Ubiquitin thiolesterase CYLD; Ubiquitin-specific processing protease CYLD; Ubiquitin-specific-processing protease CYLD; USPL2
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Deubiquitinase that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation
Gene References into Functions
  1. A substantial number of CLL patient samples express sCYLD. PMID: 28566736
  2. No genotype-phenotype correlation was found in tumor disorders of the skin appendages associated with mutations in the CYLD gene. PMID: 29974194
  3. Catalytic domain mutation in CYLD gene is associated with osteosarcoma. PMID: 29807073
  4. Studied the role of maternally expressed 3 (lncRNA-MEG3) as an antitumor lncRNA for malignant melanoma by regulating miR-499-5p/CYLD axis. PMID: 29808164
  5. CYLD inhibits post-transcriptional regulation of RIG-I and MDA5 expression following TLR3 activation in MCs. CYLD may be involved in the pathogenesis of CKD PMID: 29166644
  6. miR-197-3p-induced downregulation of CYLD promotes cell proliferation and inhibits cell apoptosis in lung adenocarcinoma cell lines. PMID: 29286108
  7. Knockdown of CYLD expression reversed the cell proliferation promotion by miR1288in in glioblastoma. PMID: 28901464
  8. TNF phase III signalling displays ongoing TNFR1/NF-kappa B activation in monocytic cells. High- and low-sensitive genes are induced including differentially regulated A20. A20 strictly controls this signalling in an IKK- and partially RIP-dependent manner. The A20-mediated control mechanisms are supported by ABIN1 and CYLD. PMID: 28629782
  9. Low expression of CYLD is associated with glioma. PMID: 28534976
  10. theses data show that CYLD regulates the magnitude of ubiquitination of several major effectors of the EGFR pathway by assisting the recruitment of the ubiquitin ligase Cbl-b to the activated EGFR complex. PMID: 28572092
  11. Through deceasing the expression of Cylindromatosis (CYLD), a K63-specific DUB and endogenous blocker of NF-kappaB signaling, miR-130b can in return sustain the persistent activation of NF-kappaB, which may promote the malignant progression of transitional cell carcinoma of bladder. PMID: 27391066
  12. While previous reports have described CYLD as a regulator of DVL proteins; this data suggests the presence of a more complicated reciprocal regulatory mechanism in CML cell lines. PMID: 28840581
  13. Thrombin-mediated MALT1 protease activation triggers acute disruption of endothelial barrier integrity via CYLD cleavage. PMID: 27681433
  14. MiR-767 acted as a role of tumor promoter by targeting CYLD in human melanoma. PMID: 29054757
  15. SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes. PMID: 27545878
  16. The data reveal SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling. PMID: 27591049
  17. The current investigations identified a subset of HPV-positive HNSCCs with mutations in the genes TRAF3 (tumor necrosis factor receptor-associated factor 3) and CYLD (cylindromatosis lysine 63 deubiquitinase). Defects in TRAF3 and CYLD correlated with the activation of transcriptional factor nuclear factor kappaB, episomal HPV status of tumors, and improved patient survival. PMID: 28295222
  18. The predicted PUB domain in the N-terminus of SPATA2 interacts with the USP domain of CYLD and SPATA2 is required for recruitment of CYLD to the TNF-alpha receptor-associated signaling complexes. PMID: 27307491
  19. STAT3 and miR-181b control each other's expression in a positive feedback loop that regulates SFCs via CYLD pathway. PMID: 27189061
  20. CYLD Promotes TNF-alpha-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells PMID: 27738385
  21. Low CYLD expression is associated with hepatocellular carcinoma. PMID: 28184924
  22. miR20a directly repressed the expression of CYLD, leading to activation of the NFkappaB pathway and the downstream targets, livin and survivin, which potentially induced GC chemoresistance. PMID: 27357419
  23. Data show that cylindromatosis (CYLD) overexpression and livin knockdown might improve gemcitabine chemosensitivity by decreasing autophagy and increasing apoptosis in bladder cancer (BCa) cells. PMID: 27448305
  24. aberrantly expressed miR-130b may regulate cell apoptosis and proliferation of human gastric cancer cells via CYLD, which appears to be a promising therapeutic target for gastric cancer PMID: 26711782
  25. These results demonstrate the involvement of histone deacetylases in the down regulation of Cyld expression in hepatocellular carcinoma cells. PMID: 27570065
  26. Novel CYLD germline mutations c.1821_1826+1delinsCT/L607Ffs*9, c.2666A>T/p.D889V and c.2712delT/p.905Kfs*8 were identified in unrelated Brooke-Spiegler Syndrome patients. PMID: 26329847
  27. Study founds the levels of CYLD and SMAD7 significantly decreased in oral squamous cell carcinoma (OSSC) cells and proposes a model that CYLD suppresses OSCC metastases through SMAD7. Downregulation of CYLD appears to directly contribute to the distal metastases of primary OSCC and subsequently poor prognosis. PMID: 26982322
  28. Haplotype analysis was performed for the patients with multiple familial trichoepithelioma type 1, patients with familial cylindromatosis and a patient with Brooke-Spiegler syndrome, all of whom carry the same heterozygous nonsense CYLD mutation. PMID: 26861065
  29. Ultraviolet radiation induced CYLD translocation from the cytoplasm to microtubules, posttranslational modification and degradation in a proteasome-independent manner. PMID: 26717101
  30. Deubiquitinase CYLD negatively regulates MyD88-mediated signaling by directly interacting with MyD88 and deubiquitinating nontypeable Haemophilus influenzae (NTHi)-induced K63-linked polyubiquitination of MyD88 at lysine 231. PMID: 26719415
  31. CYLD interrupts the ERK- and p38-/AP-1 and c-Myc pathways to suppress Nrf2-operated antioxidative capacity, thereby enhancing oxidative stress in the heart. PMID: 25935309
  32. Data suggest OPTN (optineurin) is involved in up-regulation of innate immunity in mitosis; mechanism involves phosphorylation/nuclear translocation of CYLD and phosphorylation/mitochondrial translocation of TBK1 (NF-kB-activating kinase). PMID: 25923723
  33. CaMKII-mediated recruitment and upregulation of CYLD is expected to remove K63-linked polyubiquitins and facilitate proteasomal degradation at the postsynaptic density. PMID: 24614225
  34. The deubiquitinating enzyme CYLD controls apical docking of basal bodies in ciliated epithelial cells. PMID: 25134987
  35. Phenotype-genotype correlations for clinical variants caused by CYLD mutations. [Review] PMID: 25782638
  36. we identified a novel mutation of the CYLD gene in a Chinese multiple familial trichoepithelioma family. PMID: 25117167
  37. Low CYLD expression is associated with colorectal cancer. PMID: 25824771
  38. Expression of nuclear CYLD is a novel prognostic factor for improved survival in patients with HCC undergoing liver resection or transplantation. PMID: 25329885
  39. A recurrent R936X nonsense mutation of CYLD was identified in a large Chinese family with multiple familial trichoepithelioma. PMID: 25751345
  40. Clinical management of patients with germline CYLD mutations is challenging and we discuss genetic counselling and surgical interventions PMID: 26370355
  41. Data show that inhibiting the expression of cylindromatosis (CYLD) gene can suppress the proliferation and migration of umbilical vein endothelial cells (HUVECs). PMID: 26271973
  42. TRAF-6 mediates S. p-induced PAI-1 expression, and CYLD inhibits PAI-1 expression probably through deubiquitinating TRAF-6 PMID: 26056944
  43. DC-SIGN directs adaptive T helper cell type-2immunity to fucose-expressing pathogens via an IKKepsilon-CYLD-dependent signalling pathway leading to Bcl3 activation. PMID: 24867235
  44. Here, we report a novel interaction between CYLD and MIB2 that supports the hypothesis that the CYLD/MIB2 interaction might play a pathogenic role in human cancer. PMID: 25565632
  45. A heterozygous missense mutation (c.1112C>A) in the 9th exon of the CYLD gene was detected in some mother-daughter patients with multiple trichoepitheliomas. PMID: 25501176
  46. CYLD displayed its ability to restrict the cells in G2 phase of cell cycle PMID: 25641919
  47. the 35kDa CYLD cleaved factor generated by MALT-1 mediated proteolytic cleavage was conspicuously present in human T- ALL subjects of pediatric age group. PMID: 25130432
  48. CYLD negatively regulates nontypeable Haemophilus influenzae-induced IL-8 expression via MKP-1-dependent inhibition of ERK. PMID: 25389768
  49. Overexpression of non-SUMOylatable mutant CYLD in neuroblastoma cells reduced retinoic acid-induced NF-kappaB activation and differentiation of cells, but instead promoted cell death. PMID: 24909169
  50. Report a novel heterozygous frameshift mutation c.1169_1170delCA (p.Thr390Argfs) of the CYLD gene in Chinese family with multiple familial trichoepithelioma. PMID: 25234269

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Involvement in disease Cylindromatosis, familial (FCYL); Multiple familial trichoepithelioma 1 (MFT1); Brooke-Spiegler syndrome (BRSS)
Subcellular Location Cytoplasm, Cytoplasm, perinuclear region, Cytoplasm, cytoskeleton, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, cilium basal body
Protein Families Peptidase C19 family
Tissue Specificity Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney.
Database Links

HGNC: 2584

OMIM: 132700

KEGG: hsa:1540

STRING: 9606.ENSP00000308928

UniGene: Hs.578973

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