Recombinant Mouse Alsin (Als2), partial

Code CSB-YP835624MO
MSDS
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Source Yeast
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Code CSB-EP835624MO
MSDS
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Source E.coli
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Code CSB-EP835624MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835624MO
MSDS
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Source Baculovirus
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Code CSB-MP835624MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Als2
Uniprot No.
Alternative Names
Als2; Alsin; Amyotrophic lateral sclerosis 2 protein homolog
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.
Gene References into Functions
  1. two ALS-linked factors, SQSTM1 and ALS2, have distinct but additive protective roles against mutant SOD1-mediated toxicity by modulating neuronal proteostasis possibly through the autophagy-endolysosomal system. PMID: 27439389
  2. By taking advantage of this CSMN reporter line of AlsinKO, authors reveal that apical dendrite degeneration is a common cellular pathology for CSMN that become diseased for different causes. PMID: 26755825
  3. alsin and spartin may interact each other physically. PMID: 22982304
  4. Alsin and SOD1(G93A) proteins regulate endosomal reactive oxygen species production by glial cells and proinflammatory pathways responsible for neurotoxicity. PMID: 21937428
  5. these results suggest that Als2 is a binding partner of Uxt and Als2/Uxt interaction could be important for the activation of Nf-kappaB pathway. PMID: 21907703
  6. Rab5-mediated endocytosis was severely altered in ALS2(-/-) neurons. PMID: 19630956
  7. ALS2/Alsin exacerbates motor dysfunction in a SOD1-expressing mouse ALS model by disturbing endolysosomal trafficking PMID: 20339559
  8. Als2(-/-) mice showed a significantly lower spontaneous rearing activity than wild-type litters. These genetic background- and/or gender-specific findings suggest the presence of modifiers for life span and motor activities in Als2(-/-) mice. PMID: 20558214
  9. Alsin is a Rab5 and Rac1 guanine nucleotide exchange factor PMID: 15033976
  10. Rac1, PI3 kinase, and Akt3 have roles in an anti-apoptotic pathway triggered by ALS2 that antagonizes SOD1 mutant-induced motoneuronal cell death PMID: 15579468
  11. loss of ALS2 function is not sufficient to cause motor neuron disease in a mouse model. However, lack of ALS2 did predispose neurons to oxidative stress, implying that ALS2 might serve as a risk factor for motor neuron disease. PMID: 16107644
  12. The homozygous deletion in exon 4 of the ALS2 gene (553delA). PMID: 16240357
  13. Mice deficient in the Rab5 guanine nucleotide exchange factor ALS2/alsin exhibit age-dependent neurological deficits and altered endosome trafficking. PMID: 16321985
  14. Altered trophic receptor trafficking in neurons of Als2(-/-) mice may underlie the histopathological and behavioral changes observed and the pathogenesis of ALS2 PMID: 16769894
  15. deficiency in ALS2 causes an upper motor neuron disease that closely resembles a severe form of hereditary spastic paralysis, and that is quite distinct from amyotrophic lateral sclerosis. PMID: 16802286
  16. alsin controls the growth and survival of motoneurons in a Rac1-dependant manner PMID: 16802292
  17. Our data suggest that deficiency in the ALS2 gene does not affect the pathogenesis of SOD1(G93A) mice. PMID: 16973244
  18. Novel function of ALS2 gene in lymphopoiesis and hematopoiesis, suggesting that immune system is involved in pathogenesis of one form of juvenile onset autosomal recessive amyotrophic lateral sclerosis(ALS2). PMID: 17156857
  19. ALS2 is a novel Rac1 effector and is involved in Rac1-activated macropinocytosis. PMID: 17409386
  20. alsin-deficient mice have motor impairment and degenerative pathology in the distal corticospinal tracts without apparent motor neuron pathology PMID: 17855450
  21. Mouse models fail to recapitulate hallmarks of motor neuron disease, but the subtle deficits are observed in behavior and pathology. [RREVIEW] PMID: 17955197
  22. ALS2 may act as a modulator in neuronal differentiation and/or development through regulation of membrane dynamics. PMID: 18358238
  23. The Als2(-/-) mice lacking exon 2 and part of exon 3 developed mild signs of neurodegeneration compatible with axonal transport deficiency. PMID: 18558633
  24. Als2-depleted spinal motor neurons can be rescued by co-cultured astrocytes. PMID: 19304783

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