Recombinant Mouse Apoptotic protease-activating factor 1(Apaf1) ,partial

Code CSB-YP001885MO
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Source Yeast
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Code CSB-EP001885MO
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Source E.coli
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Code CSB-EP001885MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001885MO
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Source Baculovirus
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Code CSB-MP001885MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Apaf1
Uniprot No. O88879
Alternative Names Apaf1Apoptotic protease-activating factor 1; APAF-1
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity).
Gene References into Functions
  1. data collectively demonstrated that Apaf1 is a negative regulator of T cell responses and implicated Apaf1 as a potential target for immunosuppressive drug discovery PMID: 29596528
  2. miR-183 was induced in protective postconditioning and reduced reperfusion injury of the livers via the targeting of apoptotic signaling. miR-183 mediated the tolerance induced by iPoC in livers via Apaf-1 repressing. PMID: 27580417
  3. Apaf1's pivotal role during embryonic development as has been demonstrated to be in regulation of neuronal cell death PMID: 26374523
  4. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
  5. Apaf1 is required for proper cortical neuron differentiation since its deletion specifically impairs axonal outgrowth. PMID: 25975226
  6. study demonstrates that MMP-3 leads to caspase-9 activation and suggests that this occurs indirectly via a cytosolic protein, possibly involving Apaf-1 PMID: 25285627
  7. results provide evidence that Apaf-1 pharmacological inhibition has therapeutic potential for the treatment of apoptosis-related diseases. PMID: 25330150
  8. Study reports experimental evidence suggesting the likely participation of the Apaf1 protein in the aggregation of polyQ stretches; Apaf1 may enhance polyglutamine aggregation by reducing the cellular protein levels of available functional Hsp70 PMID: 24412373
  9. miR-23a/b and miR-27a/b are endogenous inhibitory factors of Apaf-1 expression and regulate the sensitivity of neurons to apoptosis. PMID: 24651435
  10. a role for Apaf1 at the mitochondria PMID: 24416260
  11. The Apaf-1-independent caspase activation was not inhibited by Bcl-2, and occurred without the release of cytochrome c from mitochondria, suggesting that the mitochondrial pathway was not involved in this death process. PMID: 23197294
  12. These data are consistent with a model in which alterations of GH signaling and/or insulin sensitivity lead to increased lifespan mediated by decreased renal expression of pro-apoptotic genes. PMID: 21431351
  13. we have for the first time demonstrated the critical role of Apaf-1 in the regulation of MAP kinases, rective oxygen species and mitochondrial membrane potential in UVC-radiated fibroblasts. PMID: 22226830
  14. plays a pro-survival role by regulating centrosome morphology and function PMID: 21984814
  15. Crystal structure of full-length Apaf-1, proper function of Apaf-1 relies on R265 in the vicinity of the bound nucleotide PMID: 21827944
  16. Ku70/86 binds to the Apaf1 promoter and represses its activity. PMID: 20966962
  17. Findings suggest that Apaf-1/caspase-9-mediated nonapoptotic caspase signaling is required for the proper neural network formation during olfactory development. PMID: 20624980
  18. Non-apoptotic cell death of neural progenitor cells may compensate for APAF-1 deficiency in developing central nervous systems. PMID: 19914347
  19. A drug targeting Apaf-1 allows protection from apoptosis as well as regeneration in the course of inflammation-induced tissue injury PMID: 19675677
  20. Apaf-1 plays a critical role in apoptosis in a subset of tissues and that both E2F1:p53:Apaf-1-dependent and -independent apoptotic pathways operate downstream of Rb. PMID: 11731416
  21. The Apaf-1-mediated pathway of apoptosis is dispensable for negative selection of thymocytes PMID: 11859117
  22. Apaf1 reduced expression levels generate a mutant phenotype in adult brain and skeleton of mice. PMID: 11859416
  23. Apaf-1(-/-) (null) fibroblasts were resistant to cell death initiated by proapoptotic stimuli such as tamoxifen; susceptible to apoptosis induced by thapsigargin and brefeldin-A, both of which induce ER stress PMID: 11919205
  24. apoptotic cells show perinuclear cytochrome c aggregation, which may be critical for nuclear redistribution of cytochrome c and Apaf-1. PMID: 12062423
  25. Apaf-1 is not involved in positive selection or death by neglect, but may have a partial role in negative selection during early thymic T-cell development. PMID: 12121437
  26. Bcl-2 regulates a caspase activation programme independently of the cytochrome c/Apaf-1/caspase-9 'apoptosome', which seems to amplify rather than initiate the caspase cascade PMID: 12374983
  27. Apaf-1 has been detected in developing kidney cells and may be required for caspase-9 activation in kidney organogenesis. PMID: 12660414
  28. APAF-1 has a nonessential role in apoptosis PMID: 12773531
  29. p53 regulation of Apaf-1 expression is tissue-specific PMID: 12883670
  30. Apaf-1 and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation. PMID: 14709542
  31. Coupling of caspase-9 to Apaf1 in apoptotic pathways is cell-type-specific. PMID: 14713951
  32. the cytochrome c-Apaf-1-procaspase-9 complex functions in the caspase amplification rather than in its initiation PMID: 14747474
  33. disruption of the Apaf1 gene led to a decrease in apoptosis in the inner ear epithelium, morphogenetic defects and a size reduction of the membranous labyrinth, demonstrating an Apaf1-dependent apoptotic pathway is necessary for inner ear development PMID: 15105372
  34. Loss of Apaf-1 or caspase-9 would be unlikely to enhance survival of cancer cells. PMID: 15210730
  35. Apaf-1/pro-caspase-9 complex is recruited by Nucling for the induction of stress-induced apoptosis PMID: 15271982
  36. Hsp72 inhibits apoptosis upstream of the mitochondria and not through interactions with Apaf-1 PMID: 15371421
  37. loss of HAND2 in the pharyngeal arch mesenchyme leads to apoptosis in an Apaf-1-dependent fashion PMID: 15649468
  38. These studies indicate the existence of a Cyt c- and apoptosome-independent but Apaf-1-dependent mechanism(s) for caspase activation. PMID: 15907471
  39. The data indicate that ultraviolet-C light activates in mouse embryonic fibroblasts both the Fas (CD95, Apo-1) receptor and the mitochondrial damage pathways. PMID: 16215690
  40. neuroprotective effects of Hsp70 overexpression in neonatal hypoxic/ischemic injury are mediated by apoptotic pathways through upregulation of FLIP and sequestering Apaf-1, leading to reduced cleavage of caspase-8 and caspase-9 PMID: 16397188
  41. progression of UV-induced apoptosis requires IRES-mediated translation of Apaf-1 to ensure continuous levels of Apaf-1 despite an overall suppression of protein synthesis PMID: 16595687
  42. Apaf-1 and the mitochondrial pathway of apoptosis play significant roles in ER stress-induced apoptosis. PMID: 16954146
  43. These findings identify a critical function of Bax in committing neurons to endoplasmic reticulum stress-induced apoptosis and clarify the importance of the apoptosome as the non-redundant caspase activation pathway. PMID: 17218955
  44. Data support a model in which Apaf-1 is necessary for the cleavage or activation of all procaspases and the promotion of mitochondrial apoptotic events induced by genotoxic drugs. PMID: 17348858
  45. Caspase 9 activation in response to intrinsic apoptotic stimuli can be uncoupled from Apaf-1 in vivo by XIAP antagonists. PMID: 17562856
  46. Apaf-1 and caspase-9 deficiency prevents apoptosis in a Bax-controlled pathway and promotes clonogenic survival during paclitaxel treatment. PMID: 17652622
  47. These data point to a role for Apaf-1 as a bona fide tumor suppressor. PMID: 18042457
  48. As neurons mature, cytochrome c- mediated apoptosis progresses from inhibitor of apoptosis protein-dependent to -independent regulation because of a complete loss of Apaf-1 expression. PMID: 18056406
  49. Survival study of mature postmitotic neuronal populations in mice in which Apaf-1 has been genetically deleted reveals quantitatively normal programmed cell death of developing postmitotic neurons. PMID: 18256270
  50. Data show that Faf1 protein level is down-regulated during apoptosis in a caspase-3- and Apaf1-dependent manner. PMID: 18480964

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Subcellular Location Cytoplasm
Tissue Specificity Highly expressed in lung and spleen, weakly in brain and kidney and not detectable in liver.
Database Links

KEGG: mmu:11783

STRING: 10090.ENSMUSP00000020157

UniGene: Mm.220289

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