Recombinant Mouse Aromatic-L-amino-acid decarboxylase (Ddc)

Code CSB-YP006583MO
MSDS
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Source Yeast
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Code CSB-EP006583MO
MSDS
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Source E.coli
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Code CSB-EP006583MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006583MO
MSDS
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Source Baculovirus
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Code CSB-MP006583MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ddc
Uniprot No.
Alternative Names
DdcAromatic-L-amino-acid decarboxylase; AADC; EC 4.1.1.28; DOPA decarboxylase; DDC
Species
Mus musculus (Mouse)
Expression Region
1-480
Target Protein Sequence
MDSREFRRRG KEMVDYIADY LDGIEGRPVY PDVEPGYLRP LIPATAPQEP ETYEDIIKDI EKIIMPGVTH WHSPYFFAYF PTASSYPAML ADMLCGAIGC IGFSWAASPA CTELETVMMD WLGKMLELPE AFLAGRAGEG GGVIQGSASE ATLVALLAAR TKVIRQLQAA SPEFTQAAIM EKLVAYTSDQ AHSSVERAGL IGGIKLKAVP SDGNFSMRAS ALREALERDK AAGLIPFFVV ATLGTTSCCS FDNLLEVGPI CNQEGVWLHI DAAYAGSAFI CPEFRYLLNG VEFADSFNFN PHKWLLVNFD CSAMWVKRRT DLTGAFNMDP VYLKHSHQDS GFITDYRHWQ IPLGRRFRSL KMWFVFRMYG VKGLQAYIRK HVELSHEFES LVRQDPRFEI CTEVILGLVC FRLKGSNELN ETLLQRINSA KKIHLVPCRL RDKFVLRFAV CARTVESAHV QLAWEHISDL ASSVLRAEKE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
Gene References into Functions
  1. We found that only the modified U1 snRNA (IVS-AAA) that completely matched both the intronic and exonic U1 binding sequences of the mutated DDC gene could correct splicing errors of either the mutated human DDC minigene or the mouse artificial splicing construct in vitro PMID: 27658936
  2. The data show that endothelial AADC plays an important role in cardiac synthesis of 5-HT and possibly in 5-HT-dependent regulation of nitric oxide generation. PMID: 22829864
  3. Mash1 plays an important role in regulating the expression of AADC in type III cells in taste buds. PMID: 21322090
  4. evidence for PKGIalpha-dependent phosphorylation and activation of neuronal AAAD in vitro, and introduce AAAD as a putative PKGIalpha substrate. PMID: 20456015
  5. Data suggest a possible participation of aromatic L-amino acid decarboxylase (AADC) in the regulation of catecholamine biosynthesis in stellate ganglia of repeatedly stressed mice. PMID: 15207355
  6. Results suggest that AADC expression begins at early stages of taste bud cell differentiation, and biogenic amines may act on taste bud differentiation of tongue epithelial cells, and further may regulate innervation of taste bud progenitor cells. PMID: 17211625
  7. analysis of Ddc redefines the imprinted Grb10 domain on mouse proximal chromosome 11 and identifies Ddc_exon1a as the first example of a heart-specific imprinted gene PMID: 17967881
  8. the fact that renal DDC inversely correlated with renal sodium reabsorption suggest that renal and intestinal gender dimorphism in DDC could be related to sex-related differences in sodium balance observed between males and females PMID: 18310457

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Protein Families
Group II decarboxylase family
Database Links
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