Recombinant Mouse Axin-1(Axin1) ,partial

Code CSB-YP520310MO
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Source Yeast
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Code CSB-EP520310MO
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Source E.coli
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Code CSB-EP520310MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP520310MO
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Source Baculovirus
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Code CSB-MP520310MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Axin1
Uniprot No. O35625
Alternative Names Axin1; Axin; FuAxin-1; Axis inhibition protein 1; Protein Fused
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
Gene References into Functions
  1. Axin-1 is critical for spindle organization and cell cycle progression during meiotic maturation in mouse oocytes. PMID: 27284927
  2. VDR is important for the maintenance of physiological level of Axin1 PMID: 27601169
  3. Axin1 promotes dendritic spine stabilization through Cdc42-dependent cytoskeletal reorganization. PMID: 26204446
  4. Axin1 and Axin2 do not have equivalent functions in satellite cells, but are both involved in repression of Wnt/beta-catenin signalling to maintain proliferation and contribute to controlling timely myogenic differentiation. PMID: 25866367
  5. Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature. PMID: 25336178
  6. propose that disrupted Fu function in these structures underlies the head and flagellar defects in Fu-deficient sperm PMID: 24525297
  7. Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799
  8. This study demonistrated that Axin directs the amplification and differentiation of intermediate progenitors in the developing cerebral cortex in mice. PMID: 23972596
  9. results suggestg HSV-1 replication enhancement might be mediated by the axin RGS domain. PMID: 23535572
  10. an asymmetrical distribution pattern for Axin1 transcripts in 2-cell embryos. PMID: 22071115
  11. Conditional disruption of Axin1 leads to development of liver tumors in mice. PMID: 22960659
  12. The Axin/TNKS2/KIF3A complex is critical for GLUT4 translocation in response to insulin. PMID: 22473005
  13. Phosphorylation of axin-1 by glycogen synthase kinase-3beta accelerates association of axin-1 with beta-catenin in dorsal root ganglion growth cones. PMID: 22933777
  14. Axin1 apparently has a preventive effect on bacterial invasiveness and inflammatory response during the early stages of infection. The results suggest a distinct biological function of Axin1 and Axin2 in infectious disease and intestinal inflammation PMID: 22509369
  15. phosphorylation of Axin and its interaction with GSK-3beta are critically required for axon formation in mouse cortex development. PMID: 21940452
  16. Axin1 expression renders L929 cells sensitive to Aurora inhibition-induced cell death in a PARP- and AIF-dependent manner. PMID: 21520248
  17. show that AXIN interacts with PML in vivo, and further that AXIN, PML and p53 form a ternary complex PMID: 21057547
  18. Axin(Fu) mutation induces alternative splicing and ultimately leads to abnormal tail development in mice. There is a potential connection between alternative splicing with the expressivity and penetrance of the mutant allele. PMID: 11703504
  19. CKI and CKII exhibit differential effects on Axin-MEKK1 interaction and Axin-mediated JNK activation; elucidation of the functional switch of Axin PMID: 11884395
  20. sumoylation plays a role for Axin to function in the JNK pathway PMID: 12223491
  21. Ectopic expression of this protein blocks neuronal differentiation of embryonic carcinoma P19 cells. PMID: 12569091
  22. murine Axin(Fu) does not undergo epigenetic reprogramming during gametogenesis, and epigenetic inheritance is influenced by strain background PMID: 12601169
  23. A signaling pathway involving Axin and GSK-3, but not beta-catenin, regulates Li(+)-induced neurite outgrowth in Neuro-2a cells. PMID: 14664817
  24. highly conserved protein-protein interaction site in the axin protein PMID: 15820042
  25. Axin was shown to be fuctionally equivalent to axin 2. PMID: 15899843
  26. Fu-deficient mice do not exhibit any embryonic phenotypes indicative of perturbed Hh signaling PMID: 16055716
  27. the mFu homologue is not required for Hh signaling during embryonic development but is required for proper postnatal development, possibly by regulating the CSF homeostasis or ciliary function PMID: 16055717
  28. The activation of p38 was dependent on Axin and was required for the enhancement of mesenchymal stem cells differentiation by Wnt-4. PMID: 17720811
  29. Axin inhibits EGF-induced proliferation of L929 fibroblasts by blocking Akt activation. PMID: 17934337
  30. Both Fz and Dvl functions are critical for Wnt-induced Lrp6 phosphorylation through Fz-Lrp6 interaction. Axin, a key scaffolding protein in the Wnt pathway, is required for Lrp6 phosphorylation via its ability to recruit Gsk3. PMID: 18077588
  31. Axins act to integrate signals between the Wnt/beta-catenin and TGF-beta/Smad pathways PMID: 18083923
  32. altered JNK signaling contributes, at least in part, to the developmental defects seen in Axin(Fu) mice PMID: 18316368
  33. the C6 motif seems to reduce the interaction of Axin with Dvl-1. PMID: 18632848
  34. Wnt pathway inhibitors, Axin (intracellular) or Dickkopf-1 (extracellular) blocked the regulation of VCAM-1 by diffusible Wnt3a. PMID: 18951693
  35. These data indicate that a previously unrecognized N-cadherin-axin-LRP5 interaction negatively regulates Wnt/beta-catenin signaling and is critical in the regulation of osteoblast function, bone formation, and bone mass. PMID: 19075000
  36. Zbed3 is a novel Axin-binding protein that is involved in Wnt/beta-catenin signaling modulation. PMID: 19141611
  37. Both the RGS domain and the six C-terminal amino acids of mouse Axin are required for normal embryogenesis. PMID: 19204372
  38. Axin expression is not affected by ablation of ghrelin receptors in aging mice, suggesting a ghrelin-independent mechanism for regulation of axin. PMID: 19299626
  39. Results suggest that Axin modulates distribution of Axin-associated proteins in an expression level-dependent manner and these interactions affect the mitotic process. PMID: 19331826
  40. Dab2 stabilizes Axin and attenuates Wnt/beta-catenin signaling by preventing PP1 from binding Axin PMID: 19581931
  41. Axin is a critical determinant in p53-dependent tumour suppression in which Pirh2 and Tip60 have different roles in triggering cell-cycle arrest or apoptosis depending on the severity of genotoxic stress. PMID: 19731416

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Subcellular Location Cytoplasm, Nucleus, Cell membrane, Membrane
Tissue Specificity Expressed in embryonic stem cells.
Database Links

KEGG: mmu:12005

STRING: 10090.ENSMUSP00000073974

UniGene: Mm.23684

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