Recombinant Mouse CCN family member 2(Ccn2),partial

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Code CSB-EP006147MOe0
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Ccn2
Uniprot No. P29268
Research Area Cardiovascular
Alternative Names Ccn2; betaIG-M2; Ctgf; Fisp-12; Fisp12; Hcs24; CCN family member 2; Cellular communication network factor 2; Connective tissue growth factor; Hypertrophic chondrocyte-specific protein 24; Protein FISP-12
Species Mus musculus (Mouse)
Source E.coli
Expression Region 26-344aa
Target Protein Sequence QDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.0kDa
Protein Length Partial
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).
Gene References into Functions
  1. These results reveal a novel mechanism of macrophage migration into glomeruli with nephritis mediated by connective tissue growth factor derived from mesangial cells. PMID: 28191821
  2. early myocardial CTGF mRNA expression (six hours) after Ang-II exposure is likely dependent on latent TGF-beta activation via the canonical Smad-dependent pathway in resident cardiac cells. PMID: 29575960
  3. These results indicate that the hepatocytic expression of TGF-beta and CTGF is mediated by Wnt signalling in Schistosoma japonicum infection. PMID: 28331224
  4. Data (including data from studies using transgenic mice) suggest that Ctgf secreted from vascular endothelium in pancreas plays critical role in up-regulation of insulin secretion in pancreatic beta-cells during pregnancy; here, pregnant mice with global Ctgf haplo-insufficiency (heterozygous for loss-of-function mutation) (a) exhibit impairment in maternal beta-cell proliferation and (b) develop gestational diabetes. PMID: 29111856
  5. The data demonstrate that MMP13 and CTGF play a crucial role in modulation of fibrogenic mediators while promoting hepatic fibrogenesis. PMID: 28782260
  6. p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF exp p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF expression during tooth development. PMID: 28825193
  7. Ctgf is the direct target gene of SOX9 in chondrocytes and nucleus pulposus cells. PMID: 27436052
  8. As shown in mouse model of kidney fibrosis CTGF is significantly involved in fibrosis-associated renal lymphangiogenesis through regulation of, and direct interaction with, VEGF-C. PMID: 28545716
  9. this study suggested that CTGF antibody protected podocytes against injury in DN mice by reducing beta-catenin overexpression and preventing podocyte EMT, which might provide new insight into the mechanism of CTGF inhibition in the treatment of DN. PMID: 28370212
  10. LPA-LPA1 signaling initiates profibrotic epithelial cell fibroblast communication mediated by epithelial cell derived connective tissue growth factor. PMID: 27927603
  11. Down-regulation of CTGF is effective in inhibiting postoperative scarring in vivo. This suggests that RNAi with CTGF siRNA may potentially pave the road for a novel therapeutic strategy to improve glaucoma surgery results. PMID: 26554857
  12. CTGF role in cardiac fibrosis. Long noncoding RNA H19 mediates CTGF expression. PMID: 28753062
  13. Notch1 haploinsufficiency decreased the expression of Ctgf in the aorta and in vitro cell culture system. In vitro studies on SMCs using the Notch1 intracellular domain (NICD) plasmid, dominant negative mastermind-like (dnMAML), or specific siRNA suggest that Notch1, not Notch3, directly modulates the expression of CTGF PMID: 28562688
  14. these findings show that cardiac ERK1/2 activity is modulated in part by TGF-b/Smad signaling, leading to altered activation of CTGF/CCN2 to mediate fibrosis and alter cardiac function. This identifies a novel mechanism in the development of LMNA cardiomyopathy. PMID: 27131347
  15. the mechanistic target of rapamycin (mTOR) pathway in neurons regulates CTGF production and secretion, revealing a paracrine mechanism by which neuronal signaling regulates oligodendrocyte maturation and myelination in tuberous sclerosis complex (TSC). PMID: 28183733
  16. CCN2 suppression by miR-199a-5p accounts, in part, for low-level fibrogenic gene expression in quiescent hepatic stellate cells (HSCs) and causes dampened gene expression in activated HSCs after horizontal transfer of miR-199a-5p in exosomes from quiescent HSCs. PMID: 27662798
  17. Overexpression of CTGF in cardiomyocytes attenuates left ventricular hypertrophy in angiotensin II induced hypertension. PMID: 28287886
  18. CTGF is overexpressed in gastric cancer and adjacent tissue compared to normal gastric tissue. Gastrin induces expression of CTGF in gastric epithelial cells. PMID: 27179776
  19. These results indicate that tensile force induces in vivo gene expression associated with vascularization early in tensile-force-induced sutural bone formation. Moreover, the early induction of Vegf gene expression is regulated by CTGF and ROCK2. PMID: 26825658
  20. these results suggested that loss of miR-30c may contribute to the pathogenesis of diabetic nephropathy (DN) by inhibiting target CTGF expression; replenishing miR-30c may ameliorate renal structure and function by reducing renal fibrosis in DN. PMID: 26775556
  21. Data suggest that PTEN phosphatase (PTEN) regulates renal extracellular matrix production via activated protein kinase B (Akt) and increased connective tissue growth factor (CTGF) in diabetes mellitus. PMID: 26447680
  22. Under physiological conditions, CCN2 may be regulating the levels of free amino acids in the extracellular matrix of cartilage. PMID: 26364758
  23. These data indicate that LPA increases CCN2 expression through the activation of PKC and PKA. Thus, the regulatory functions of the PKC and PKA pathways are implicated in the LPA-induced increase in CCN2 expression PMID: 26743816
  24. CTGF inhibition may benefit patients with dilated cardiomyopathy. PMID: 26549358
  25. These results suggest that TGF-beta1 and CTGF may be involved in the process of denervated skeletal muscle fibrosis. PMID: 26686381
  26. CCN genes are activated in heart failure. PMID: 25712640
  27. CTGF knockout does not affect cardiac hypertrophy and fibrosis formation upon chronic pressure overload. PMID: 26410398
  28. these findings reveal a key role of the SRF/CTGF/miR-133a axis in the regulation of cardiac fibrosis PMID: 26440278
  29. Descriptive Statement Elimination of TGF-betaIIR is not sufficient to completely prevent liver fibrosis. TGF-beta-independent mechanism of type I collagen production and suggest connective tissue growth factor as its potent mediator. PMID: 25108224
  30. CCN2 up regulation by tumor stromal cells is responsible for the melanoma metastasis. PMID: 26168233
  31. CTGF siRNA ameliorates tubular cell apoptosis and tubulointerstitial fibrosis in obstructed mouse kidneys in a Sirt1-independent manner. PMID: 26257513
  32. CTGF overexpression induces epithelial to mesenchymal transition in mouse primary AT II cells, which is mediated by ILK PMID: 25580742
  33. Data suggest that, in mice with type 1 diabetes mellitus and diabetic nephropathy, up-regulation of urinary CTGF level is independently associated with biomarkers of proximal and distal tubular dysfunction and pathology. PMID: 26171399
  34. Report showed that loss of PTEN expression resulted in collagen deposition in the lung in a CCN2-dependent manner supporting the notion that alterations in PTEN expression contribute to fibrogenesis and that CCN2 mediates collagen production in fibrosis. PMID: 25644104
  35. knockdown of MRTF-A synthesis abolishes the systemic sclerosis myofibroblast enhanced basal contractility and synthesis of type I collagen and inhibits the matricellular profibrotic protein, connective tissue growth factor CCN2/CTGF PMID: 25955164
  36. findings suggest CCN2 as a candidate of the fourth factor in the RANK/RANKL/OPG system for osteoclastogenesis, which regulates OPG and RANK via direct interaction PMID: 25554597
  37. MRTF-A promotes microvessel growth (via CCN1) and maturation (via CCN2), thereby enabling functional improvement of ischaemic muscle tissue PMID: 24910328
  38. CTGF reporter mice selectively identify a subpopulation of bone marrow mesenchymal progenitor cells that reside in the trabecular bone region. PMID: 25464947
  39. Neither heart-specific Ctgf deletion nor CTGF overexpression altered cardiac remodeling and function with aging or after multiple acute stress stimuli. PMID: 25870108
  40. This study provides a better understanding of the functions of connective tissue growth factor within the bone marrow, showing the dual regulatory role of the growth factor in skeletogenesis and in stage-specific B lymphopoiesis. PMID: 24727816
  41. functional connection between CTGF and integrin alphavbeta6 during hepatic progenitor/oval cell activation and associated fibrosis PMID: 25203810
  42. Ctgf is a target of Notch canonical signaling in osteoblasts, and may act in concert with Notch to regulate skeletal homeostasis PMID: 24792956
  43. application of exogenous CCN2 protein caused ERK1/2 activation, and the neutralizing CCN2 antibody inhibited loading-induced ERK1/2 activation. PMID: 24155087
  44. ET1 contributes to AF-dependent atrial fibrosis by synergistic activity with AngII to stimulate SGK1 expression and enhance phosphorylation of the SGK1 protein which, in turn, induces CTGF. PMID: 23414741
  45. CTGF attenuates cardiac hypertrophy upon chronic pressure-overload due to inhibition of signaling mechanisms that promote pathologic myocardial hypertrophy. PMID: 23452880
  46. CCN2 is packaged into secreted, nano-sized exosomes that mediate its intercellular transfer between hepatic stellate cells. PMID: 24882759
  47. Transverse aortic constriction induced the cardiac expression of profibrotic connective tissue growth factor and attenuated the expression of SERCA2a (sarcoplasmic reticulum Ca2+-ATPase) in knockout mice PMID: 25027872
  48. These findings uncover a novel functional role of CCN2 as a metabolic supporter in the growth-plate chondrocytes, which is required for skeletogenesis in mammals. PMID: 24288211
  49. In a cardiomyocyte cell line, CTGF was secreted in response to cell stretching on a flexible membrane base (a cellular phenotype of cardiac diastolic dysfunction). PMID: 24464932
  50. CCN2 expression by dermal papillae cells is a physiologically relevant suppressor of hair follicle formation by destabilization of beta-catenin and CCN2 normally acts to maintain stem cell quiescence. PMID: 24152728
  51. Reducing CTGF/CCN2 slows down mdx muscle dystrophy and improves cell therapy. PMID: 23904456
  52. Data indicate that connective tissue growth factor (CTGF) is expressed in cardiomyocytes and cardiac Fibroblasts. PMID: 24688123
  53. Loss of ACE2 exacerbates AngII-mediated inflammation, myocardial injury and dysfunction in ACE2-deficient hearts via activation of the CTGF-FKN-ERK and MMP signaling. PMID: 24161906
  54. show that ROS accumulation correlates with media layer degeneration and increased connective tissue growth factor (CTGF) expression PMID: 23985903
  55. Deletion of CTGF had an attenuated fibrotic response to bleomycin. PMID: 24508728
  56. Connective tissue growth factor is expressed in the naive cornea, lens, iris, and retina, and is expressed immediately after epithelial injury. Loss of CTGF impairs efficient re-epithelialization of corneal wounds. PMID: 24627144
  57. Demonstrate a novel role for CTGF/CCN2 during pancreatitis and its ability to modulate pro-inflammatory pathways. PMID: 24754049
  58. Exosomal transfer of miR-214 is a paradigm for the regulation of CCN2-dependent fibrogenesis and identifies fibrotic pathways as targets of intercellular regulation by exosomal miRs. PMID: 24122827
  59. The PDZ-binding motif of YAP is critical for YAP-mediated oncogenesis, and that this effect is mediated by YAP's co-activation of TEAD-mediated CTGF transcription. PMID: 24380865
  60. CTGF is involved in structural retinal vascular changes in diabetic rodents. PMID: 24217924
  61. genetic variability in CTGF expression directly modulates the severity of diabetic nephropathy. PMID: 23950936
  62. CCN family member 2/connective tissue growth factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes. PMID: 23951098
  63. Impaired intervertebral disc development and premature disc degeneration develops in mice with notochord-specific deletion of CCN2. PMID: 23839921
  64. ACE inhibition might improve muscle strength and decrease fibrosis by diminishing specifically CTGF expression and activity without affecting TGF-beta1 signaling. PMID: 23673415
  65. Loss of CCN2 resulted in resistance to the increases in collagen production and myofibroblast recruitment that are caused by loss of PTEN. PMID: 23983074
  66. promotes epithelium-to-mesenchyma transition morphological changes in fibroblasts PMID: 23525012
  67. CCN2 plays a key role in extracellular matrix gene expression in severe hypertrophic cardiomyopathy and heart failure. PMID: 23756156
  68. CCN-2 plays a role in adipocyte differentiation in vivo and thus in the pathogenesis of obesity linked with insulin resistance. PMID: 23571711
  69. this study uncovers a novel mechanism controlling beta-adrenergic receptor responsiveness in cardiomyocytes involving connective tissue growth factor mediated regulation of GRK5. PMID: 23778361
  70. This study showed that the odorant-specific recruitment of distinct glomeruli resulted in enhanced local CTGF expression levels in the activated glomeruli. Collectively our data reveal a molecular PMID: 23993699
  71. suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
  72. Ctgf is a downstream target of the Smad-dependent TGF-beta signaling pathway in the developing palate. PMID: 23816882
  73. The data suggest an important role of CTGF in mesenchymal stromal cells differentiation into adipocytes and of leptin in homing and progression of leukemia. PMID: 23741006
  74. Cartilage-specific over-expression of CCN2 stimulates insulin-like growth factor expression and bone growth. PMID: 23555635
  75. CTGF contributes to fibrocyte proliferation in the myocardium and enhances fibrocyte differentiation into a myofibroblast phenotype responsible for extracellular matrix deposition. PMID: 23287510
  76. findings reveal a negative feedback loop between CCN2 and HIF-1alpha in NP cells and demonstrate a role for CCN2 in maintaining matrix homeostasis in this tissue. PMID: 23530034
  77. LPA1-induced cytoskeleton reorganization makes a critically important contribution to the profibrotic activities of LPA by driving MRTF-dependent CTGF expression, which, in turn, drives fibroblast proliferation PMID: 23322166
  78. Increased myocardial CTGF activities after MI are associated with attenuation of LV remodeling and improved LV function mediated by attenuation of inflammatory responses and inhibition of apoptosis. PMID: 23284892
  79. Hemizygous deletion of CTGF/CCN2 does not suffice to prevent fibrosis of the severely injured kidney. PMID: 23199377
  80. The skeletal site-specific role of connective tissue growth factor in prenatal osteogenesis. PMID: 23073844
  81. Subcutaneously implanted, MSC-loaded polyvinyl alcohol (PVA) sponges and stented excisional wounds were used as wound models to study the dynamics of CTGF expression. PMID: 23212098
  82. CTGF is an important mediator of high glucose-induced podocyte damage and decreases the protein level of podocalyxin by the ERK1/2 pathway. PMID: 21602138
  83. CTGF regulates retinal neovascularization through p53 protein-dependent transactivation of the MMP-2 gene. PMID: 23048035
  84. CTGF but not TGFb1 mRNA expression in the liver is significantly related to the duration of infection in schistosomal hepatic fibrosis. PMID: 22913183
  85. SRF plays a key role in the modulation of cardiac fibrosis through repression of cardiomyocyte CTGF expression in a paracrine fashion. PMID: 22563064
  86. Bladder remodeling corresponding to experimental autoimmune encephalomyelitis severity may be due to enhanced expression of CTGF and increased growth of connective tissue. PMID: 22993071
  87. It conclude that in the murine atria, CCN1 and CCN2 proteins are expressed constitutively. In chronic heart failure, CCN proteins tend to be upregulated, which may be related to the action of angiotensin II. PMID: 22532143
  88. These results provided mechanistic insight into the angiogenic action of CCN2/CTGF and demonstrated the therapeutic potential of dominant-negative CCN2/CTGF mutants for antiangiogenesis. PMID: 22611085
  89. The MAPKs/TGFb1/TRAF6 pathway is an important signaling pathway in AngII-induced CTGF expression. PMID: 22749815
  90. Connective tissue growth factor causes glaucoma by modifying the actin cytoskeleton of the trabecular meshwork. PMID: 22542845
  91. CCN2 is required for the normal production of vascular basement membranes. PMID: 22363445
  92. CTGF and ILK were involved in high glucose-induced phenotypic alterations of podocytes. ILK acted as a downstream kinase of CTGF and high glucose-induced ILK expression might occur through CTGF-dependent and -independent pathways. PMID: 21913214
  93. The increase of CTGF expression was associated with the severity of myocardial fibrosis in viral myocarditis. PMID: 22321187
  94. These findings demonstrate that CTGF is a downstream mediator in TGF-beta and progesterone signaling cascades and is necessary for normal follicle development and ovulation. PMID: 21868453
  95. Ctgf is important for vessel growth during early retinal development and promotes the fibrovascular reaction in murine retinal ischemia after laser injury. PMID: 21969300
  96. CTGF acts downstream of muscle injury and also contributes directly to the deterioration of skeletal muscle phenotype and function in skeletal muscle dystrophy. PMID: 21826667
  97. these findings support the chemoattractive role of CCN2 for P19 cells, but not its adhesive role when compared to laminin or fibronectin. PMID: 21925146
  98. findings suggest CCN2 regulates integrin expression in primary culture hepatic stellate cells (HSC) and supports HSC adhesion via its binding of cell surface integrin alpha(5)beta(1) PMID: 20406330
  99. SPARC and connective tissue growth factor appeared to be involved in the same biological pathway PMID: 21978691
  100. Overexpression of PTEN reduces the overexpression of type I collagen and CCN2 by dSSc fibroblasts PMID: 21654839

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Subcellular Location Secreted, extracellular space, extracellular matrix, Secreted
Protein Families CCN family
Tissue Specificity Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).
Database Links

KEGG: mmu:14219

STRING: 10090.ENSMUSP00000020171

UniGene: Mm.390287

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