Mouse connective tissue growth factor,CTGF ELISA Kit

Code CSB-E07877m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CTGF ELISA Kit was designed for the quantitative measurement of Mouse CTGF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL .

Target Name connective tissue growth factor
Alternative Names Ccn2 ELISA Kit; betaIG-M2 ELISA Kit; Ctgf ELISA Kit; Fisp-12 ELISA Kit; Fisp12 ELISA Kit; Hcs24 ELISA Kit; CCN family member 2 ELISA Kit; Cellular communication network factor 2 ELISA Kit; Connective tissue growth factor ELISA Kit; Hypertrophic chondrocyte-specific protein 24 ELISA Kit; Protein FISP-12 ELISA Kit
Abbreviation CTGF
Uniprot No. P29268
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 92  
Range % 87-96  
1:200 Average % 95  
Range % 90-100  
1:400 Average % 87  
Range % 82-93  
1:800 Average % 90  
Range % 84-96  
Recovery
The recovery of mouse CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-102  
EDTA plasma (n=4) 94 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.597 2.580 2.589 2.412  
25 2.263 2.298 2.281 2.104  
12.5 1.779 1.799 1.789 1.612  
6.25 1.217 1.252 1.235 1.058  
3.12 0.690 0.674 0.682 0.505  
1.56 0.458 0.427 0.443 0.266  
0.78 0.285 0.297 0.291 0.114  
0 0.176 0.178 0.177    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Gene References into Functions
  1. These results reveal a novel mechanism of macrophage migration into glomeruli with nephritis mediated by connective tissue growth factor derived from mesangial cells. PMID: 28191821
  2. early myocardial CTGF mRNA expression (six hours) after Ang-II exposure is likely dependent on latent TGF-beta activation via the canonical Smad-dependent pathway in resident cardiac cells. PMID: 29575960
  3. These results indicate that the hepatocytic expression of TGF-beta and CTGF is mediated by Wnt signalling in Schistosoma japonicum infection. PMID: 28331224
  4. Data (including data from studies using transgenic mice) suggest that Ctgf secreted from vascular endothelium in pancreas plays critical role in up-regulation of insulin secretion in pancreatic beta-cells during pregnancy; here, pregnant mice with global Ctgf haplo-insufficiency (heterozygous for loss-of-function mutation) (a) exhibit impairment in maternal beta-cell proliferation and (b) develop gestational diabetes. PMID: 29111856
  5. The data demonstrate that MMP13 and CTGF play a crucial role in modulation of fibrogenic mediators while promoting hepatic fibrogenesis. PMID: 28782260
  6. p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF exp p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF expression during tooth development. PMID: 28825193
  7. Ctgf is the direct target gene of SOX9 in chondrocytes and nucleus pulposus cells. PMID: 27436052
  8. As shown in mouse model of kidney fibrosis CTGF is significantly involved in fibrosis-associated renal lymphangiogenesis through regulation of, and direct interaction with, VEGF-C. PMID: 28545716
  9. this study suggested that CTGF antibody protected podocytes against injury in DN mice by reducing beta-catenin overexpression and preventing podocyte EMT, which might provide new insight into the mechanism of CTGF inhibition in the treatment of DN. PMID: 28370212
  10. LPA-LPA1 signaling initiates profibrotic epithelial cell fibroblast communication mediated by epithelial cell derived connective tissue growth factor. PMID: 27927603
  11. Down-regulation of CTGF is effective in inhibiting postoperative scarring in vivo. This suggests that RNAi with CTGF siRNA may potentially pave the road for a novel therapeutic strategy to improve glaucoma surgery results. PMID: 26554857
  12. CTGF role in cardiac fibrosis. Long noncoding RNA H19 mediates CTGF expression. PMID: 28753062
  13. Notch1 haploinsufficiency decreased the expression of Ctgf in the aorta and in vitro cell culture system. In vitro studies on SMCs using the Notch1 intracellular domain (NICD) plasmid, dominant negative mastermind-like (dnMAML), or specific siRNA suggest that Notch1, not Notch3, directly modulates the expression of CTGF PMID: 28562688
  14. these findings show that cardiac ERK1/2 activity is modulated in part by TGF-b/Smad signaling, leading to altered activation of CTGF/CCN2 to mediate fibrosis and alter cardiac function. This identifies a novel mechanism in the development of LMNA cardiomyopathy. PMID: 27131347
  15. the mechanistic target of rapamycin (mTOR) pathway in neurons regulates CTGF production and secretion, revealing a paracrine mechanism by which neuronal signaling regulates oligodendrocyte maturation and myelination in tuberous sclerosis complex (TSC). PMID: 28183733
  16. CCN2 suppression by miR-199a-5p accounts, in part, for low-level fibrogenic gene expression in quiescent hepatic stellate cells (HSCs) and causes dampened gene expression in activated HSCs after horizontal transfer of miR-199a-5p in exosomes from quiescent HSCs. PMID: 27662798
  17. Overexpression of CTGF in cardiomyocytes attenuates left ventricular hypertrophy in angiotensin II induced hypertension. PMID: 28287886
  18. CTGF is overexpressed in gastric cancer and adjacent tissue compared to normal gastric tissue. Gastrin induces expression of CTGF in gastric epithelial cells. PMID: 27179776
  19. These results indicate that tensile force induces in vivo gene expression associated with vascularization early in tensile-force-induced sutural bone formation. Moreover, the early induction of Vegf gene expression is regulated by CTGF and ROCK2. PMID: 26825658
  20. these results suggested that loss of miR-30c may contribute to the pathogenesis of diabetic nephropathy (DN) by inhibiting target CTGF expression; replenishing miR-30c may ameliorate renal structure and function by reducing renal fibrosis in DN. PMID: 26775556
  21. Data suggest that PTEN phosphatase (PTEN) regulates renal extracellular matrix production via activated protein kinase B (Akt) and increased connective tissue growth factor (CTGF) in diabetes mellitus. PMID: 26447680
  22. Under physiological conditions, CCN2 may be regulating the levels of free amino acids in the extracellular matrix of cartilage. PMID: 26364758
  23. These data indicate that LPA increases CCN2 expression through the activation of PKC and PKA. Thus, the regulatory functions of the PKC and PKA pathways are implicated in the LPA-induced increase in CCN2 expression PMID: 26743816
  24. CTGF inhibition may benefit patients with dilated cardiomyopathy. PMID: 26549358
  25. These results suggest that TGF-beta1 and CTGF may be involved in the process of denervated skeletal muscle fibrosis. PMID: 26686381
  26. CCN genes are activated in heart failure. PMID: 25712640
  27. CTGF knockout does not affect cardiac hypertrophy and fibrosis formation upon chronic pressure overload. PMID: 26410398
  28. these findings reveal a key role of the SRF/CTGF/miR-133a axis in the regulation of cardiac fibrosis PMID: 26440278
  29. Descriptive Statement Elimination of TGF-betaIIR is not sufficient to completely prevent liver fibrosis. TGF-beta-independent mechanism of type I collagen production and suggest connective tissue growth factor as its potent mediator. PMID: 25108224
  30. CCN2 up regulation by tumor stromal cells is responsible for the melanoma metastasis. PMID: 26168233
  31. CTGF siRNA ameliorates tubular cell apoptosis and tubulointerstitial fibrosis in obstructed mouse kidneys in a Sirt1-independent manner. PMID: 26257513
  32. CTGF overexpression induces epithelial to mesenchymal transition in mouse primary AT II cells, which is mediated by ILK PMID: 25580742
  33. Data suggest that, in mice with type 1 diabetes mellitus and diabetic nephropathy, up-regulation of urinary CTGF level is independently associated with biomarkers of proximal and distal tubular dysfunction and pathology. PMID: 26171399
  34. Report showed that loss of PTEN expression resulted in collagen deposition in the lung in a CCN2-dependent manner supporting the notion that alterations in PTEN expression contribute to fibrogenesis and that CCN2 mediates collagen production in fibrosis. PMID: 25644104
  35. knockdown of MRTF-A synthesis abolishes the systemic sclerosis myofibroblast enhanced basal contractility and synthesis of type I collagen and inhibits the matricellular profibrotic protein, connective tissue growth factor CCN2/CTGF PMID: 25955164
  36. findings suggest CCN2 as a candidate of the fourth factor in the RANK/RANKL/OPG system for osteoclastogenesis, which regulates OPG and RANK via direct interaction PMID: 25554597
  37. MRTF-A promotes microvessel growth (via CCN1) and maturation (via CCN2), thereby enabling functional improvement of ischaemic muscle tissue PMID: 24910328
  38. CTGF reporter mice selectively identify a subpopulation of bone marrow mesenchymal progenitor cells that reside in the trabecular bone region. PMID: 25464947
  39. Neither heart-specific Ctgf deletion nor CTGF overexpression altered cardiac remodeling and function with aging or after multiple acute stress stimuli. PMID: 25870108
  40. This study provides a better understanding of the functions of connective tissue growth factor within the bone marrow, showing the dual regulatory role of the growth factor in skeletogenesis and in stage-specific B lymphopoiesis. PMID: 24727816
  41. functional connection between CTGF and integrin alphavbeta6 during hepatic progenitor/oval cell activation and associated fibrosis PMID: 25203810
  42. Ctgf is a target of Notch canonical signaling in osteoblasts, and may act in concert with Notch to regulate skeletal homeostasis PMID: 24792956
  43. application of exogenous CCN2 protein caused ERK1/2 activation, and the neutralizing CCN2 antibody inhibited loading-induced ERK1/2 activation. PMID: 24155087
  44. ET1 contributes to AF-dependent atrial fibrosis by synergistic activity with AngII to stimulate SGK1 expression and enhance phosphorylation of the SGK1 protein which, in turn, induces CTGF. PMID: 23414741
  45. CTGF attenuates cardiac hypertrophy upon chronic pressure-overload due to inhibition of signaling mechanisms that promote pathologic myocardial hypertrophy. PMID: 23452880
  46. CCN2 is packaged into secreted, nano-sized exosomes that mediate its intercellular transfer between hepatic stellate cells. PMID: 24882759
  47. Transverse aortic constriction induced the cardiac expression of profibrotic connective tissue growth factor and attenuated the expression of SERCA2a (sarcoplasmic reticulum Ca2+-ATPase) in knockout mice PMID: 25027872
  48. These findings uncover a novel functional role of CCN2 as a metabolic supporter in the growth-plate chondrocytes, which is required for skeletogenesis in mammals. PMID: 24288211
  49. In a cardiomyocyte cell line, CTGF was secreted in response to cell stretching on a flexible membrane base (a cellular phenotype of cardiac diastolic dysfunction). PMID: 24464932
  50. CCN2 expression by dermal papillae cells is a physiologically relevant suppressor of hair follicle formation by destabilization of beta-catenin and CCN2 normally acts to maintain stem cell quiescence. PMID: 24152728

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Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families CCN family
Tissue Specificity Testis, spleen, kidney, lung, heart, and brain (lowest level in testis and highest in lung).
Database Links

KEGG: mmu:14219

STRING: 10090.ENSMUSP00000020171

UniGene: Mm.390287

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