Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
GbaLysosomal acid glucosylceramidase; Lysosomal acid GCase; EC 3.2.1.45; Acid beta-glucosidase; Beta-glucocerebrosidase; Cholesterol glucosyltransferase; SGTase; EC 2.4.1.-; Cholesteryl-beta-glucosidase; EC 3.2.1.104; D-glucosyl-N-acylsphingosine glucohydrolase
Species
Mus musculus (Mouse)
Expression Region
20-515aa
Target Protein Sequence
AQPCIPKSFGYSSVVCVCNASYCDSLDPVTLPALGTFSRYESTRRGRRMELSVGAIQANRTGTGLLLTLQPEKKFQKVKGFGGAMTDATALNILALSPPTQKLLLRSYFSTNGIEYNIIRVPMASCDFSIRVYTYADTPNDFQLSNFSLPEEDTKLKIPLIHQALKMSSRPISLFASPWTSPTWLKTNGRVNGKGSLKGQPGDIFHQTWANYFVKFLDAYAKYGLRFWAVTAENEPTAGLFTGYPFQCLGFTPEHQRDFISRDLGPALANSSHDVKLLMLDDQRLLLPRWAEVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPIIVDIPKDAFYKQPMFYHLGHFSKFIPEGSQRVALVASESTDLETVALLRPDGSAVVVVLNRSSEDVPLTISDPDLGFLETVSPGYSIHTYLWRRQ
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Full-length of mature Mouse Lysosomal acid Glucosylceramidase (Gba) with a 6xHis-tag at the N-terminus is produced in the yeast. It contains 20-515aa of mouse Gba. The SDS-PAGE measured its purity by up to 90%. On the reducing SDS-PAGE gel, the Gba protein migrated to a band of approximately 65 kDa. In addition to being used to produce specific antibodies, this recombinant Gba protein also may be applied to study its catalytic substrate-related metabolism.
Gba is a lysosomal enzyme that catalyzes the hydrolysis of glucosylceramide into ceramide and glucose. Genetic deficiency of Gba results in the accumulation of glucosylceramide in the lysosomes of macrophages, inducing the transformation of macrophages into Gaucher cells. The Gaucher cells infiltrate various organs such as bone marrow, spleen, and liver and are responsible for Gaucher's disease (GD), a recessively inherited disorder that affects the central nervous system to varying degrees.