Recombinant Full-length of mature Mouse Lysosomal acid Glucosylceramidase (Gba) with a 6xHis-tag at the N-terminus is produced in the yeast. It contains 20-515aa of mouse Gba. The SDS-PAGE measured its purity by up to 90%. On the reducing SDS-PAGE gel, the Gba protein migrated to a band of approximately 65 kDa. In addition to being used to produce specific antibodies, this recombinant Gba protein also may be applied to study its catalytic substrate-related metabolism.
Gba is a lysosomal enzyme that catalyzes the hydrolysis of glucosylceramide into ceramide and glucose. Genetic deficiency of Gba results in the accumulation of glucosylceramide in the lysosomes of macrophages, inducing the transformation of macrophages into Gaucher cells. The Gaucher cells infiltrate various organs such as bone marrow, spleen, and liver and are responsible for Gaucher's disease (GD), a recessively inherited disorder that affects the central nervous system to varying degrees.
||Greater than 90% as determined by SDS-PAGE.
||GbaLysosomal acid glucosylceramidase; Lysosomal acid GCase; EC 188.8.131.52; Acid beta-glucosidase; Beta-glucocerebrosidase; Cholesterol glucosyltransferase; SGTase; EC 2.4.1.-; Cholesteryl-beta-glucosidase; EC 184.108.40.206; D-glucosyl-N-acylsphingosine glucohydrolase
||Mus musculus (Mouse)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
||Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.