Recombinant Mouse Nidogen-1(Nid1),partial

In Stock
Code CSB-YP015802MOe1
Size US$860
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details


The first step of making this recombinant Mouse Nid1 protein is synthesizing the Nid1 gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has Tag-Free encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant Nid1 protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.

Nid1, also known as entactin-1, is a glycoprotein found in basement membranes. It functions as a linker between laminins, collagens, and proteoglycans in the extracellular matrix and binds to cell surface integrins, assisting in the establishment and maintenance of the basement membrane and tissue architecture. Nid1 also regulates cell polarization, migration, and invasion through interactions with cell receptor molecules. Braconi et al. reported that Nid1 is upregulated in hepatocellular cancer with vascular invasion by whole-genome analyses. Zhang Y et al. discovered that Nid1 was an ovarian cancer-associated protein and can be detectable in peripheral blood, with increased expression in ovarian cancer patients. Later, Nid1 was found to hold the potential to be a helpful biomarker in the diagnosis of ovarian serious carcinoma.

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Nid1
Uniprot No. P10493
Research Area Others
Alternative Names
Nid1; Ent; Nidogen-1; NID-1; Entactin
Species Mus musculus (Mouse)
Source Yeast
Expression Region 428-665aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 26.3kDa
Protein Length Partial
Tag Info Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Gene References into Functions
  1. Analysis of nidogen-1 and laminin-gamma1 interaction reveals multiple binding modes. PMID: 25387007
  2. The C-terminal domains of mouse laminin gamma 1 and nidogen 1 mediate interactions complex formation. PMID: 23948589
  3. nidogen-1 is essential to provide an anti-angiogenic environment of differentiated vessels. PMID: 24280453
  4. finding demonstrates that nidogens, although homologous proteins, exert their effect on BM assembly through different binding domains, which may in turn result in alterations of BM structure and functions PMID: 22623588
  5. show that loss of nidogen 2, but not of nidogen 1, promotes lung metastasis of melanoma cells. Histological and ultrastructural analysis of nidogen 1- and 2-deficient lungs did not reveal differences in morphology and ultrastructure of basement membrane PMID: 22260998
  6. Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain. PMID: 21084308
  7. This study demonstrates that nidogen-1 is essential for maintenance of normal hippocampal excitability and synaptic plasticity. PMID: 19530222
  8. Results suggest a non-structural role for nidogen 1 in wound repair. PMID: 19766719
  9. Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development. PMID: 12015298
  10. These findings revealed extensive coregulation of nidogen-1 and -2 expression and much more complementary functions of the two nidogens than previously recognized. PMID: 12243745
  11. Three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene. PMID: 12475645
  12. structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5) PMID: 12931195
  13. Expression may promote formation of sinusoids after partial hepatectomy. PMID: 15777791
  14. Laminin gamma3 chain, located in murine basement membranes, binds to nidogen PMID: 15824114
  15. Nid1 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue PMID: 16024816
  16. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone PMID: 17008882
  17. nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud PMID: 17023412
  18. expression of the entactin-1 dramatically declined in myogenesis PMID: 17177854
  19. The lack of nidogen-1, nidogen-2, or both nidogens, plays no crucial role in the occurrence and localization of laminin-111, collagen type IV, and perlecan in murine tubular renal basement membranes. PMID: 17616934
  20. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411. PMID: 18356808

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane.
Database Links

KEGG: mmu:18073

STRING: 10090.ENSMUSP00000005532

UniGene: Mm.4691

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