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The first step of making this recombinant Mouse Nid1 protein is synthesizing the Nid1 gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has Tag-Free encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant Nid1 protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.Nid1, also known as entactin-1, is a glycoprotein found in basement membranes. It functions as a linker between laminins, collagens, and proteoglycans in the extracellular matrix and binds to cell surface integrins, assisting in the establishment and maintenance of the basement membrane and tissue architecture. Nid1 also regulates cell polarization, migration, and invasion through interactions with cell receptor molecules. Braconi et al. reported that Nid1 is upregulated in hepatocellular cancer with vascular invasion by whole-genome analyses. Zhang Y et al. discovered that Nid1 was an ovarian cancer-associated protein and can be detectable in peripheral blood, with increased expression in ovarian cancer patients. Later, Nid1 was found to hold the potential to be a helpful biomarker in the diagnosis of ovarian serious carcinoma.
|Purity||Greater than 90% as determined by SDS-PAGE.|
Nid1; Ent; Nidogen-1; NID-1; Entactin
|Species||Mus musculus (Mouse)|
|Target Protein Sequence||SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
|Gene References into Functions||
|Subcellular Location||Secreted, extracellular space, extracellular matrix, basement membrane.|