Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Nid1

Uniprot No.

P10493

Research Area

Others

Alternative Names

Nid1; Ent; Nidogen-1; NID-1; Entactin

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

428-665aa

Target Protein Sequence

SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

26.3kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Unlock the potential of your research with the Recombinant Mouse Nid1 protein. This protein closely mirrors the native Nidogen-1 (also known as NID-1 and Entactin) present in Mouse species, making it an ideal candidate for varied research applications.

This recombinant protein covers a partial expression region from 428-665 amino acids, precisely cloned and expressed in a yeast system for optimal functionality. For the convenience of detection and minimal interference with the protein’s activity, the protein is tag-free, allowing for studies in conditions mirroring the natural biological environment.

The Recombinant Mouse Nid1 protein assures high reliability in your experiments, with purity greater than 90% as confirmed by SDS-PAGE. It is available in both liquid and lyophilized powder forms, providing you the flexibility to choose the right format for your experiments.

Target Background

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Gene References into Functions

Analysis of nidogen-1 and laminin-gamma1 interaction reveals multiple binding modes. PMID: 25387007
The C-terminal domains of mouse laminin gamma 1 and nidogen 1 mediate interactions complex formation. PMID: 23948589
nidogen-1 is essential to provide an anti-angiogenic environment of differentiated vessels. PMID: 24280453
finding demonstrates that nidogens, although homologous proteins, exert their effect on BM assembly through different binding domains, which may in turn result in alterations of BM structure and functions PMID: 22623588
show that loss of nidogen 2, but not of nidogen 1, promotes lung metastasis of melanoma cells. Histological and ultrastructural analysis of nidogen 1- and 2-deficient lungs did not reveal differences in morphology and ultrastructure of basement membrane PMID: 22260998
Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain. PMID: 21084308
This study demonstrates that nidogen-1 is essential for maintenance of normal hippocampal excitability and synaptic plasticity. PMID: 19530222
Results suggest a non-structural role for nidogen 1 in wound repair. PMID: 19766719
Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development. PMID: 12015298
These findings revealed extensive coregulation of nidogen-1 and -2 expression and much more complementary functions of the two nidogens than previously recognized. PMID: 12243745
Three- to sevenfold increase in nidogen-2 was observed in heart and muscle of mice with nidogen-1 deficiency. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene. PMID: 12475645
structure of the interacting domains reveals a six-bladed Tyr-Trp-Thr-Asp (YWTD) beta-propeller domain in nidogen bound to laminin epidermal-growth-factor-like (LE) modules III3-5 in laminin (LE3-5) PMID: 12931195
Expression may promote formation of sinusoids after partial hepatectomy. PMID: 15777791
Laminin gamma3 chain, located in murine basement membranes, binds to nidogen PMID: 15824114
Nid1 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue PMID: 16024816
nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone PMID: 17008882
nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud PMID: 17023412
expression of the entactin-1 dramatically declined in myogenesis PMID: 17177854
The lack of nidogen-1, nidogen-2, or both nidogens, plays no crucial role in the occurrence and localization of laminin-111, collagen type IV, and perlecan in murine tubular renal basement membranes. PMID: 17616934
In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411. PMID: 18356808

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Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Database Links

KEGG: mmu:18073

STRING: 10090.ENSMUSP00000005532

UniGene: Mm.4691

Code	CSB-YP015802MOe1
Abbreviation	Recombinant Mouse Nid1 protein, partial
MSDS	MSDS Datasheet
Size	$534
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Nidogen-1 (Nid1), partial

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