Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Nid1; Ent; Nidogen-1; NID-1; Entactin
Species
Mus musculus (Mouse)
Expression Region
428-665aa
Target Protein Sequence
SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Unlock the potential of your research with the Recombinant Mouse Nid1 protein. This protein closely mirrors the native Nidogen-1 (also known as NID-1 and Entactin) present in Mouse species, making it an ideal candidate for varied research applications.
This recombinant protein covers a partial expression region from 428-665 amino acids, precisely cloned and expressed in a yeast system for optimal functionality. For the convenience of detection and minimal interference with the protein’s activity, the protein is tag-free, allowing for studies in conditions mirroring the natural biological environment.
The Recombinant Mouse Nid1 protein assures high reliability in your experiments, with purity greater than 90% as confirmed by SDS-PAGE. It is available in both liquid and lyophilized powder forms, providing you the flexibility to choose the right format for your experiments.