Recombinant Mouse Nidogen-1 (Nid1) comes from yeast expression and covers a partial sequence spanning amino acids 428 to 665. It includes an N-terminal 6xHis-tag that makes purification straightforward. The protein shows a purity level exceeding 90% when verified by SDS-PAGE, which appears to ensure high-quality results in research applications. This product is intended for research use only.
Nidogen-1 serves as an essential component of the extracellular matrix. It's primarily involved in the structural organization and stability of basement membranes. The protein plays a critical role in cell-matrix interactions and participates in various biological pathways, including tissue development and repair processes. Its importance in research likely stems from its involvement in cell adhesion and signaling, making it a key focus in studies related to tissue engineering and regenerative medicine.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Extracellular Matrix Interaction Studies
This recombinant mouse Nidogen-1 fragment (428-665aa) can help investigate protein-protein interactions within the extracellular matrix network. The expressed region may contain binding domains that interact with other ECM components such as laminin, collagen IV, or perlecan. Researchers might use this protein in binding assays, surface plasmon resonance, or co-immunoprecipitation experiments to map interaction networks. The N-terminal 6xHis tag makes purification and immobilization easier for these interaction studies.
2. Antibody Development and Validation
The recombinant Nidogen-1 fragment works well as an antigen for generating and validating antibodies specific to mouse Nidogen-1. Its high purity (>90%) and defined amino acid sequence (428-665aa) make it suitable for immunizing animals to produce polyclonal or monoclonal antibodies. This protein can also validate antibody specificity through ELISA, Western blot, or immunoprecipitation assays. The His-tag allows easy coating onto ELISA plates or immobilization for antibody screening applications.
3. Protein Structure-Function Analysis
This partial Nidogen-1 protein may prove useful in structural biology studies to understand the folding and domain organization of specific Nidogen-1 regions. Researchers can perform circular dichroism spectroscopy, dynamic light scattering, or limited proteolysis experiments to characterize the structural properties of this fragment. The defined boundaries (428-665aa) allow for systematic structure-function relationship studies by comparing this fragment with other Nidogen-1 domains or full-length protein.
4. Cell Adhesion and Migration Assays
The recombinant Nidogen-1 fragment works as a substrate in cell culture studies to examine its role in cell adhesion, spreading, and migration. Researchers can coat culture plates with this protein to assess how different cell types respond to this specific Nidogen-1 region. The His-tag makes controlled immobilization and quantification of protein coating density more manageable. These studies might provide insights into the functional significance of this particular Nidogen-1 domain in cellular processes.
