Recombinant Rat Achaete-scute homolog 1(Ascl1)

Code CSB-YP002199RA
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Source Yeast
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Code CSB-EP002199RA
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Source E.coli
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Code CSB-EP002199RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002199RA
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Source Baculovirus
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Code CSB-MP002199RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ascl1
Uniprot No. P19359
Alternative Names Ascl1; Ash1; Mash-1; Achaete-scute homolog 1
Species Rattus norvegicus (Rat)
Expression Region 1-233
Target Protein Sequence MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAAQSAQQQ QQQQAPQQQA PQLSPVADGQ PSGGGHKSAA KQVKRQRSSS PELMRCKRRL NFSGFGYSLP QQQPAAVARR NERERNRVKL VNLGFATLRE HVPNGAANKK MSKVETLRSA VEYIRALQQL LDEHDAVSAA FQAGVLSPTI SPNYSNDLNS MAGSPVSSYS SDEGSYDPLS PEEQELLDFT NWF
Protein Length Full length protein
Tag Info The following tags are available.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Transcription factor that plays a key role in neuronal differentiation
Gene References into Functions
  1. Data found that Ascl1 promoted tangential migration along the ventricular zone/subventricular zone (VZ/SVZ) and intermediate zone (IZ) of the dorsal telencephalon. Also, Ascl1 induces Ephb2 expression in the telencephalon. PMID: 28276447
  2. miR-34a appears to play an important role in the process of liver fibrosis by targeting ACSL1 and may show promise as a therapeutic molecular target for hepatic fibrosis PMID: 26437572
  3. Data demonstrate that MASH1/Ascl1a is a fundamental regulator of axonal growth across vertebrates and can induce modifications to the intrinsic state of neurons to promote functional regeneration in response to central nervous system injury. PMID: 25751153
  4. data suggest that a prominent down-regulation of hnRNP-A2/B1 during hypoxia is associated with the post-transcriptional suppression of hASH1 synthesis. PMID: 25124043
  5. This study demonistrated that over-expression of Mash1 can improve GABAergic differentiation of bone marrow mesenchymal stem cells. PMID: 24144723
  6. after FF transection, Mash1 expression in the deafferented hippocampus increased and might play an important role in inducing local progenitors to differentiate into neurons. PMID: 22583763
  7. TLX directly targets and activates the MASH1 promoter through interaction with Sp1, recruiting co-activators whereas dismissing the co-repressor HDAC4. PMID: 20599619
  8. Differentiation of cardiac ganglionic cells is affected, after RETINOIC ACID treatment, by the down-regulation of Mash-1. PMID: 11803116
  9. In cultures of neural tube-derived, primitive PNS progenitors NGNs promote expression of sensory markers and MASH1 that of autonomic markers. PMID: 11923194
  10. The expression of MASH 1 mRNA is increased in olfactory tissure from vitamin A deficiency rats PMID: 12555267
  11. The level of Mash1 mRNA increased on day 1 and then returned to the basal level by 3 days after ischemia. PMID: 15854743
  12. The Mash1 contributes to the generation of DA neurons in cooperation with Nurr1 in the developing midbrain whereas atonal-related bHLH genes inhibit the process. PMID: 16723737
  13. cooperation between Mash1 and Olig2 is necessary for neural stem/progenitor cells to develop into fully mature neurons and that down-regulation of Olig2 by Abeta in Mash1-overexpressing cells switches the cell fate to death. PMID: 17488716
  14. The present study suggests that the majority of the Mash1+ cells in the SGZ may be transit-amplifying neural progenitor cells. PMID: 17727826
  15. increased GABA neuron yield by Ngn2 and decreased yield by Mash1 were shown in the precursors of posterior brain regions PMID: 17936272
  16. Ascl1 is required for oligodendrocyte development in the spinal cord. PMID: 18287202
  17. Upon transplantation into adult rat brains, Mash1-expressing NPCs yielded large grafts enriched with neurons compared to control LacZ-transduced NPCs. PMID: 18781144
  18. Improved translation efficiency of ASH1 mRNA by FMRP may represent an important regulatory switch in neuronal differentiation. PMID: 19097999

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Subcellular Location Nucleus
Tissue Specificity Developing CNS and PNS at embryonic and post-natal stages.
Database Links

KEGG: rno:64186

STRING: 10116.ENSRNOP00000005674

UniGene: Rn.32936

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