Recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase(Eif2ak2)

Code CSB-YP723560RA
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Source Yeast
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Code CSB-EP723560RA
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Source E.coli
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Code CSB-EP723560RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723560RA
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Source Baculovirus
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Code CSB-MP723560RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Eif2ak2
Uniprot No. Q63184
Alternative Names Eif2ak2; PrkrInterferon-induced; double-stranded RNA-activated protein kinase; EC 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; Protein kinase RNA-activated; PKR; Protein kinase R; Tyrosine-protein kinase EIF2AK2; EC 2.7.10.2
Species Rattus norvegicus (Rat)
Expression Region 2-513
Target Protein Sequence ASDTPGFYV DKLNKYSQIH KVKIIYKEIS VTGPPHDRRF TFQVIIEERE FPEGEGRSKQ EAKNNAAKLA VEILDNENKV DSHTDASEQG LIEGNYIGLV NSFAQKENLP VNFELCDPDS QLPHRFICKC KIGQTTYGTG FGANKKEAKQ LAAKNAYQKL SEKSPSKTGF VTSLSSDFSS SSSITSNSAS QSASGRDFED IFMNGLREKR KSGVKVPSDD VLRNKYTLDD RFSKDFEDIE EIGSGGFGQV FKAKHRIDGK TYAIKRITYN TKKAKREVQA LAELNHANIV QYRVCWEGED YDYDPENSTN GDTSRYKTRC LFIQMEFCDK GTLQQWLEKR NRSQEDKALV LELFEQIVTG VDYIHSKGLI HRDLKPGNIF LVDEKHIKIG DFGLATALEN DGNPRTKYTG TPQYMSPEQK SSLVEYGKEV DIFALGLILA ELLHICKTDS EKIEFFQLLR NGIFSDDIFD NKEKSLLQKL LSSKPRERPN TSEILKTLAE WKNISEKKKR NTC
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses. Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3 and IRS1. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Gene References into Functions
  1. PKR plays a pivotal role in LPS-induced bone loss in periodontal disease. PMID: 27718290
  2. Zn(2+) stimulated the phosphorylation of eIF2a and promoted the nuclear accumulation of activating transcription factor 4 (ATF4). Treatment with salubrinal enhanced Zn(2+)-induced ATF4 accumulation and IL-23 p19 mRNA expression. PMID: 26174742
  3. PERK pathway is involved in oxygen-glucose-serum deprivation-induced NF-kappaB activation via reactive oxygen species generation in spinal cord astrocytes. PMID: 26454173
  4. Taken together, these data suggest that PP1-12 protects cardiomyocytes from TM- and I/R-induced apoptosis, and this effect is achieved at least in part through the inhibition of cell apoptosis and the induction of eIF2alpha phosphorylation PMID: 24366244
  5. The results suggested that the diminished hypertrophic response in the obese Zucker rat may be mediated, at least in part, by the hyperactivation of AMPK- and double-stranded-RNA-dependent protein kinase-related signaling. PMID: 22653991
  6. These results suggest that the successive events of activation of eIF2 alpha kinases and eIF2 alpha phosphorylation leading to ATF4 nuclear translocation may contribute to neuronal death. PMID: 20600673
  7. PKR is expressed in intestinal epithelial cells (IECs) as potent barriers to antigens and is a possible modulator of the differentiation of rat IECs. PMID: 20213745
  8. These results indicate that rats fed the sulfur amino acid-deficient diet had a prolonged activation of an eIF2alpha kinase that leads to upregulation of adaptive components of the integrated stress response. PMID: 20357079
  9. IFN treatment induced Mx production and increased PKR production in immature germ cells PMID: 12892903
  10. PKR activation may be involved in the mechanisms underlying ER stress-induced cell death in retinal ganglion cells PMID: 17652745
  11. Proteolytic activation of PKR may play a general role in the apoptosis of PC12 cells induced by various forms of cellular stress. PMID: 18080832
  12. Taken together with the established role of CDK activation in the promotion of neurodegeneration, our results suggest that PKRi exerts its neuroprotective action by inhibiting CDK. PMID: 19046382

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Subcellular Location Cytoplasm. Nucleus. Cytoplasm, perinuclear region.
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
Database Links

KEGG: rno:54287

STRING: 10116.ENSRNOP00000067694

UniGene: Rn.10022

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