Recombinant Rat Serine racemase (Srr)

Code CSB-YP022691RA
MSDS
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Source Yeast
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Code CSB-EP022691RA
MSDS
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Source E.coli
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Code CSB-EP022691RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022691RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022691RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Srr
Uniprot No.
Alternative Names
SrrSerine racemase; EC 5.1.1.18; D-serine ammonia-lyase; D-serine dehydratase; EC 4.3.1.18; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17
Species
Rattus norvegicus (Rat)
Expression Region
1-333
Target Protein Sequence
MCAQYCISFA DVEKAHLNIQ DSVHLTPVLT SSILNQIAGR NLFFKCELFQ KTGSFKIRGA LNAIRGLIPD TLEGKPKAVV THSSGNHGQA LTYAAKLEGI PAYIVVPQTA PNCKKLAIQA YGASIVYSEP SDESRENVAQ RIIQETEGIL VHPNQEPAVI AGQGTIALEV LNQVPLVDAL VVPVGGGGMV AGIAITIKTL KPSVKVYAAE PSNADDCYQS KLKGELTPNL HPPETIADGV KSSIGLNTWP IIRDLVDDVF TVTEDEIKYA TQLVWERMKL LIEPTAGVGL AAVLSQHFQT VSPEVKNICI VLSGGNVDLT SLSWVKQAER PAP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
Gene References into Functions
  1. Study to demonstrate that serine racemase inhibitor alleviates neuronal ischemic injury. Serine racemase inhibition acts on the neurovascular unit as a neuroprotectant and ameliorant of reductions in regional cerebral blood flow. PMID: 27693471
  2. results indicate that SRR indeed acts as a d-Asp biosynthetic enzyme in some organs and/or tissues, and also provide evidences that there should be some additional enzyme for d-Asp synthesis in mammals. PMID: 27387750
  3. In aging cerebellum, oxidative DNA stress was associated with reduced serine racemase expression. PMID: 26505919
  4. that nuclear translocation of SR provides a fail-safe mechanism to prevent or limit secondary NMDAR-mediated toxicity in nearby synapses. PMID: 26553873
  5. In serine racemase, similarly to the related enzyme alanine racemase, the unprotonated pyridoxal-5'-phosphate -substrate intermediate is stabilized mostly due to solvation effects contributed by water molecules and active-site residues. PMID: 25493718
  6. neuronal, but not astrocytic expression of serine racemase is increased at the late stage of hyposmotic challenge in vivo. PMID: 23361961
  7. serine racemase is expressed in lower esophageal sphincter PMID: 23022330
  8. The serine racemase-dependent pathway is a prime target of hippocampus-dependent cognitive deficits during aging. PMID: 19800712
  9. The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding PMID: 20106978
  10. Expression patterns of serine racemase in Schwann cells and epineural fibroblasts may indicate roles for D-serine in peripheral nerve transduction PMID: 15312798
  11. Data suggest that there is a link between the expression of serine racemase mRNA and the activity of the NMDA receptors. PMID: 15337321
  12. plays a role in biosynthesis of free D-amino acid. PMID: 18516911
  13. The srr mRNA is expressed in both neurons and glial cells of the rat retina. PMID: 18974604

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Protein Families
Serine/threonine dehydratase family
Database Links
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