Recombinant Saccharomyces cerevisiae Centractin (ARP1)

Code CSB-YP339995SVG
MSDS
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Source Yeast
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Code CSB-EP339995SVG
MSDS
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Source E.coli
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Code CSB-EP339995SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339995SVG
MSDS
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Source Baculovirus
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Code CSB-MP339995SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARP1
Uniprot No.
Alternative Names
ARP1; ACT3; ACT5; YHR129CCentractin; Actin-like protein; Actin-related protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-384
Target Protein Sequence
MDQLSDSYAL YNQPVVIDNG SGIIKAGFSG EERPKALEYC LVGNTKYDKV MLEGLQGDTF IGNNAQKLRG LLKLRYPIKH GVVEDWDSME LIWSYVLNEV LQLQNIGEHP LLITEAPMNP LKNREQMAQV LFETFDVSAL YVSNPAVLSL YASGRTTGCV VDCGEGYCST VPIYDGFALP ASMMRMDIGG ADITEQLQFQ LRKSAGVSLF SSSEREIVRT MKEKVCYLAK NIKKEEEKYL QGTQDLISTF KLPDGRCIEV GNDRYRAPEI LFSPQIIGLG YDGLSDMCMQ SIWKVDLDLR KPLLSSIILS GGTTTLKGFG DRMLWDLEAL TKGTSKIKII APSERKYTTW IGGSILTGLS TFQRLWTKKS DWLEDSTRVY SNLM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Core component of the dynactin complex which assists cytoplasmic dynein by increasing its processivity and by regulation of its cargo binding. The dynactin complex is required for the spindle translocation late in anaphase and is involved in a cell wall synthesis checkpoint. ARP1 forms the backbone filament of the dynactin rod structure and serves as the scaffold for the remaining subunits. Required for proper orientation of the mitotic spindle.
Gene References into Functions
  1. None of the ARP1 alleles disrupt filament formation; however, one pointed-end allele delayed the elution of Arp1p on gel filtration, consistent with loss of additional subunits. PMID: 15975903
  2. Analysis of 32 mutant alleles shows separation of its 2 functions, nuclear migration & the cell wall integrity checkpoint, in its molecular structure & indicate that a local surface region maintains the cell wall integrity checkpoint function. PMID: 16415535
Subcellular Location
Cytoplasm, cytoskeleton. Membrane. Note=Membrane-associated.
Protein Families
Actin family, ARP1 subfamily
Database Links

KEGG: sce:YHR129C

STRING: 4932.YHR129C

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