Recombinant Saccharomyces cerevisiae Histone acetyltransferase GCN5 (GCN5)

1. It conserved Spt-Ada-Gcn5 acetyltransferase (SAGA) complex as a paradigm to illustrate how co-activators share and combine a relatively limited set of functional tools. PMID: 28964624
2. In this study it has been demonstrated for the first time that Gcn5 and SAGA are needed for respiratory metabolism and oxygen consumption. PMID: 27741413
3. We find that RTS1, one of two genes encoding PP2A regulatory subunits, is a robust and specific high-copy suppressor of temperature sensitivity of gcn5 and a subset of other gcn5 phenotypes PMID: 27317677
4. e describe here our observation of both full length and a truncated form of a yeast protein (Gcn5) expressed in Escherichia coli, and the reduction or elimination of the truncated form by mutating a cryptic Shine-Dalgarno or START codon within the Gcn5 coding region. PMID: 26739786
5. Gcn5, Snf2, and Ydj1 cooperate in eviction of promoter nucleosomes genome-wide, achieving robust transcription of the most highly expressed subset of genes in yeast. PMID: 26602697
6. Gcn5 plays an important role in the regulatory network of FLO11 expression via Gcn4 by downregulating ICR1 expression, which derepresses FLO11 for promoting pseudohyphal development PMID: 25922832
7. the Gcn5 bromodomain contributes to lysine specificity and is necessary for processive acetylation on histone H3. PMID: 25106422
8. Acetylome profiling reveals overlap in the regulation of diverse processes by sirtuins, gcn5, and esa1 PMID: 25381059
9. NuA4 and Gcn5 enzymes are both required for the robust recruitment of SWI/SNF to a DSB, which in turn promotes the phosphorylation of H2A.X. PMID: 25869823
10. Gcn5-dependent H3K36 acetylation increases chromatin accessibility, increases resection and promotes homologous recombination DNA repair. PMID: 24909977
11. an alternative explanation for previously indicated repressive role of Gcn5 in gene transcription PMID: 23865462
12. we show that under derepressing conditions the recruitment of the histone acetyltransferase Gcn5 is abolished by SNF1 deletion, possibly explaining the lack of increased histone H3 acetylation and nucleosome remodelling PMID: 22306658
13. Data support a model in which p23 molecular chaperone and GCN5 regulate diverse multistep pathways by controlling the longevity of protein-DNA complexes. PMID: 23022381
14. We present our working model in which Elp3/Gcn5 and the APC work together to facilitate passage through mitosis and G(1). To progress into S, we propose that at least Gcn5 must then be targeted for degradation in an APC-dependent fashion. PMID: 20709786
15. Gcn5 regulates divergent sets of KCl responsive genes in S. cerevisiae and S. pombe. PMID: 20338033
16. propose that GCN5 acts as a positive regulator of DNA replication by counteracting the inhibitory effect of Histone Deacetylases PMID: 20126453
17. show that Gcn5, a KAT that functions in transcription, works in parallel with Rtt109, the H3 lysine 56 KAT, to promote replication-coupled nucleosome assembly. PMID: 20188666
18. The HatB3.1 complex is a novel histone H3-specific type B histone acetyltransferase that contains Gcn5p and Ada3p, but not Ada2p. Gcn5p appears to be the catalytic subunit of HatB3.1. PMID: 15274751
19. The importance of amino acidic residues present in the bromodomain of the histone acetyltransferase Gcn5p. PMID: 16180204
20. Gcn5 sumoylation may have an inhibitory role in transcriptional regulation PMID: 16411780
21. results show correlation between transcriptional regulation by Gcn5p and by histone H3 amino terminus indicating that H3 tail plays a small but significant positive role in transcriptional activation of Gcn5p PMID: 16461773
22. Gcn5p regulates transcription and DNA repair at the MET16 gene. PMID: 16473851
23. a role for global Gcn5 activity in modulating transcription independently of its known coactivator function PMID: 16478983
24. These investigators have characterized the genome-wide gene expression responses to KCl stress and show that Gcn5 is involved in the regulation of a subset of stress response genes. PMID: 16896217
25. The combined action of GCN5 and Abf1p determines the bidirectional capacity of the UGA3-GLT1 intergenic region. PMID: 16904075
26. Gcn5p, most likely in SAGA, stimulates modification and eviction of nucleosomes in transcribed coding sequences and promotes Pol II elongation. PMID: 17218269
27. The HATs (histone acetyltransferases) Gcn5 (general control non-derepressible 5) and Elp3 (elongation protein 3) modulated hsp70 gene transcription by affecting the acetylation status of histone H3. PMID: 17910533
28. Gcn5p is physically linked to the centromere, where it affects the structure of the variant centromeric nucleosome. PMID: 18039853
29. Rsc4p is acetylated at lysine 25 by Gcn5p PMID: 18809572
30. Data sugegst that Esa1 cooperates with Gcn5 to mediate acetylation and occupancy and histone eviction in coding sequences and stimulates the rate of transcription elongation. PMID: 19822662
31. report the discovery that Gcn5, which encodes the histone acetyltransferase (HAT) activity of the SAGA complex, has genetic interactions with the genes encoding the heterodimeric U2 snRNP proteins Msl1 and Lea1. PMID: 19834536

Show More

Hide All

KEGG: sce:YGR252W

STRING: 4932.YGR252W