|Have Questions?||Leave a Message or Start an on-line Chat|
The production of the BIRC5 recombinant antibody involves four steps, including sequencing the BIRC5 monoclonal antibody gene, cloning the gene into a plasmid vector, transfecting the recombinant vector into a host cell line, purifying the BIRC5 recombinant monoclonal antibody using affinity chromatography from the cell culture supernatant, and testing and characterizing the purified antibody. The BIRC5 monoclonal antibody is created from BIRC5 antibody-producing hybridomas, and during its production, a synthesized peptide from human BIRC5 is used as the immunogen. This BIRC5 recombinant monoclonal antibody has been recommended for ELISA and IHC applications to detect human BIRC5 protein.
BIRC5, also known as Survivin, plays an important role in regulating cell division and preventing cell death by inhibiting apoptosis. It is primarily involved in the regulation of mitosis and cytokinesis during cell division. BIRC5 is overexpressed in many human cancers and has been implicated in promoting tumor growth and progression, as well as contributing to chemotherapy resistance. In addition, BIRC5 has been shown to play a role in other cellular processes such as DNA repair, cell differentiation, and angiogenesis.
Applications : WB
Sample type: Human Cell
Review: Western bolt assays showed that the protein levels of Birc5 was promoted after shRNA-LTBP4-KD11 or KD2 was transfected into SK-MEL-1 and VMM5A cells. Data are presented as the mean ± standard deviation. ** P< 0.01