Code | CSB-RA006394A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The CYP19A1 recombinant monoclonal antibody is produced using DNA recombinant technology and in vitro genetic manipulation. Initially, an animal is immunized with a synthesized peptide derived from human CYP19A1, and B cells are isolated and screened to identify positive cells. Through PCR amplification, the light and heavy chains of the CYP19A1 antibody are obtained and inserted into a plasmid vector. This recombinant vector is then transfected into a host cell line to enable antibody expression. The CYP19A1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It exhibits specificity for human CYP19A1 protein and is well-suited for ELISA, WB, IHC, and IF applications.
The CYP19A1 protein, also known as aromatase, is an enzyme that plays a critical role in estrogen biosynthesis. In cells, the CYP19A1 protein converts androgens into estrogens, which are important for the development and maintenance of various tissues and organs, including the female reproductive system, bone, and brain. The dysregulation of CYP19A1 activity has been implicated in a number of diseases and disorders, including breast cancer, endometriosis, and osteoporosis.
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