CYP19A1 Antibody

Datasheet
Code CSB-RA006394A0HU
Size US$350
Uniprot No. P11511
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate
    All lanes: CYP19A1 antibody at 2.3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 58, 25 KDa
    Observed band size: 58 KDa

  • IHC image of CSB-RA006394A0HU diluted at 1:230 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-RA006394A0HU at 1:76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from human CYP19A1
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Relevance Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Gene Names CYP19A1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Involvement in disease Aromatase excess syndrome (AEXS); Aromatase deficiency (AROD)
Subcellular Location Membrane, Peripheral membrane protein
Protein Families Cytochrome P450 family
Tissue Specificity Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
Database Links

HGNC: 2594

OMIM: 107910

KEGG: hsa:1588

STRING: 9606.ENSP00000260433

UniGene: Hs.260074

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