E2F4 Antibody

Code CSB-RA179313A0HU
Size US$210
  • IHC image of CSB-RA179313A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of Hela cell with CSB-RA179313A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing MCF7 cells stained with CSB-RA179313A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Target Names
Alternative Names
Transcription factor E2F4 (E2F-4), E2F4
Species Reactivity
A synthesized peptide derived from human E2F4
Immunogen Species
Homo sapiens (Human)
Rabbit IgG
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
IF 1:50-1:200
FC 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The production of the E2F4 recombinant monoclonal antibody is a complex process that involves several stages. Initially, the E2F4 monoclonal antibody is obtained, and its gene sequence is determined. Then, a vector carrying the E2F4 monoclonal antibody gene is constructed and introduced into a host cell line for culture. A synthesized peptide from human E2F4 is used as the immunogen during the E2F4 monoclonal antibody production process. Finally, affinity chromatography is used to purify the resulting E2F4 recombinant monoclonal antibody, which is then evaluated for specificity via ELISA, IHC, IF, and FC applications. This E2F4 recombinant monoclonal antibody is only reactive with human E2F4 protein.

E2F4 is a transcription factor that plays a key role in cell cycle regulation and DNA replication. It binds to specific DNA sequences in target genes and either activates or represses their transcription. E2F4 is involved in the G1/S phase transition of the cell cycle and is required for proper progression through the G1 phase. It also has a role in regulating apoptosis and differentiation, and the aberrant expression or function of E2F4 has been associated with various types of cancer.

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Target Background

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Gene References into Functions
  1. E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these organizing centres and that, without cytoplasmic E2f4, deuterosomes are not assembled, halting multiciliogenesis PMID: 28675157
  2. Studied expression of E2F4 in breast cancer patients undergoing neoadjuvant chemotherapy; found target gene-based signature of E2F4 can be used to predict neoadjuvant response. PMID: 28464832
  3. The authors found that phosphorylation of residues S650 and S975 in p107 weakens the E2F4 transactivation domain binding. PMID: 27567532
  4. E2F4 gene expression in glioblastoma. PMID: 27983535
  5. This study found evidence that the number of triplet AGC repeats in the E2F4 gene may play a role in the susceptibility to early-onset colorectal cancer. PMID: 26343152
  6. PHF8 reduces the H3K9me2 level at the E2F4 transcriptional start site, demonstrating a direct function of PHF8 in endothelial E2F4 gene regulation PMID: 26751588
  7. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
  8. E2F4 promoter occupancy is globally associated with p53-repression targets, but not with p53 activation targets. PMID: 24096481
  9. cancer-associated E2F4 mutations enhance the capacity of colorectal cancer cells to grow without anchorage, thereby contributing to tumor progression. PMID: 24100580
  10. Short alleles (<13 repeats) of (AGC)n in E2F4 were less frequent in women with breast cancer than in the control sample. PMID: 23015403
  11. the loss of CDH1/E2F4 may be associated with worse clinical and pathological findings in mammary ductal carcinoma. PMID: 23007606
  12. In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component. PMID: 23093672
  13. Silica could induce the high expression of cyclin D1 and CDK4 and the low expression of E2F-4, resulting in the cell cycle changes by AP-1/cyclin D1 pathway in human embryonic lung fibroblasts. PMID: 22357515
  14. data demonstrate that enforced E2F4 expression in Burkitt lymphoma (BL) cells not only diminishes E2F1 levels, but also reduces selectively the tumorigenic properties and proliferation of BL cells PMID: 22475873
  15. Our data indicate that E2F4 is required for cardiomyocyte proliferation and suggest a function for E2F4 in mitosis PMID: 19955219
  16. E2F4, PHACTR3, PRAME family member and CDH12 most probably play important role in non-small-cell lung cancer geneses PMID: 19473719
  17. regulation of expression of p130, p107 and E2F-4 in human cells PMID: 12006580
  18. data point to Tat as an adaptor protein that recruits cellular factors such as E2F-4 to exert its multiple biological activities PMID: 12055184
  19. Mutations in E2F-4 gene is associated with hereditary non-polyposis colorectal cancer PMID: 12148576
  20. EBV LMP1 blocks p16INK4 pathway by promoting nuclear export of E2F-4. PMID: 12860972
  21. E2F-a and E2F-c binding sites are involved in the TCR-induced down-regulation of ICBP90 gene transcription PMID: 15964557
  22. E2F4 is a transcriptional regulator of the cell cycle genes. PMID: 16135806
  23. Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
  24. in response to radiation, E2F4 becomes active in the nucleus, enforces a stable G(2) arrest by target gene repression, and thus provides increased cell survival ability by minimizing propagation of cells that have irreparable DNA damage PMID: 17043659
  25. Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
  26. deregulated nuclear E2F4 expression induces apoptosis via multiple pathways in normal intestinal epithelial cells but not in colon cancer cells. PMID: 17656449
  27. E2F4, binding sites are located within 2 kb of a transcription start site, in both normal and tumor cells PMID: 17908821
  28. Cell cycle genes are the evolutionarily conserved targets of the E2F4 transcription factor PMID: 17957245
  29. FBI-1 is the first transcriptional repressor shown to act as a dual regulator in adipogenesis exerting repressor activities on target genes by both, direct and indirect mechanisms. PMID: 18368381
  30. E2F4 may be determinant in the promotion of proliferation of human intestinal epithelial crypt cells and colorectal cancer cells. PMID: 19562678
  31. By interacting with p130, E2F4 plays a key role in the maintenance of a stable G2 arrest. Increased E2F4 levels and its translocation to the nucleus following genotoxic stress result in downregulation of mitotic genes. PMID: 17507799
  32. Increased E2F4/p130 complex formation seen after irradiation depended on increased nuclear E2F4, dissociation of p130 from Cdk2, and p130 dephosphorylation. E2F4 siRNA prevented p130/E2F4 formation and sensitized cells to radiation-induced apoptosis. PMID: 15231644

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Subcellular Location
Protein Families
E2F/DP family
Tissue Specificity
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 3118

OMIM: 600659

KEGG: hsa:1874

STRING: 9606.ENSP00000368686

UniGene: Hs.108371

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