Code | CSB-RA179313A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The production of the E2F4 recombinant monoclonal antibody is a complex process that involves several stages. Initially, the E2F4 monoclonal antibody is obtained, and its gene sequence is determined. Then, a vector carrying the E2F4 monoclonal antibody gene is constructed and introduced into a host cell line for culture. A synthesized peptide from human E2F4 is used as the immunogen during the E2F4 monoclonal antibody production process. Finally, affinity chromatography is used to purify the resulting E2F4 recombinant monoclonal antibody, which is then evaluated for specificity via ELISA, IHC, IF, and FC applications. This E2F4 recombinant monoclonal antibody is only reactive with human E2F4 protein.
E2F4 is a transcription factor that plays a key role in cell cycle regulation and DNA replication. It binds to specific DNA sequences in target genes and either activates or represses their transcription. E2F4 is involved in the G1/S phase transition of the cell cycle and is required for proper progression through the G1 phase. It also has a role in regulating apoptosis and differentiation, and the aberrant expression or function of E2F4 has been associated with various types of cancer.
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