ENPEP Recombinant Monoclonal Antibody

Code CSB-RA552900A0HU
Size US$210
  • IHC image of CSB-RA552900A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
Alternative Names
Glutamyl aminopeptidase (EAP) (EC (Aminopeptidase A) (AP-A) (Differentiation antigen gp160) (CD antigen CD249), ENPEP
Species Reactivity
A synthesized peptide derived from human Aminopeptidase A / CD249
Immunogen Species
Homo sapiens (Human)
Rabbit IgG
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The process of developing the ENPEP recombinant monoclonal antibody requires several steps. Firstly, the ENPEP monoclonal antibody is harvested and its gene is sequenced. An ENPEP monoclonal antibody gene-carrying vector is constructed and then transfected into a host cell line, followed by culture. To create the ENPEP monoclonal antibody, a synthesized peptide derived from human ENPEP is used as an immunogen. The resulting ENPEP recombinant monoclonal antibody is then purified using affinity chromatography and tested for specificity via ELISA and IHC assays. IT only detects human ENPEP protein.

The ENPEP is a membrane-bound zinc metallopeptidase that plays important roles in the regulation of blood pressure, metabolism of bioactive peptides, immune response, and extracellular matrix remodeling. ENPEP is involved in the regulation of blood pressure by controlling the renin-angiotensin-aldosterone system (RAAS). ENPEP has been shown to regulate the immune response by modulating the activity of T cells and macrophages. Dysregulation of ENPEP activity has been implicated in various diseases, including hypertension, heart failure, and cancer.

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Target Background

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Gene References into Functions
  1. We suggest that APA enzymatic activity affects tumor initiation and cancer malignancy in a TWIST-dependent manner PMID: 28177885
  2. The substrate Angiotensin II, the enzymes aminopeptidases-A, B, M as well as IRAP were detected in the jejunal mucosa. PMID: 26311161
  3. This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms. PMID: 24885240
  4. These results explain the calcium-modulated substrate specificity of APA in central hypertension regulation PMID: 23888046
  5. There was no significant alteration in plasma APA activity in the patients with Chagas disease or dilated cardiomyopathies, as compared with that in health controls PMID: 21697726
  6. Rieger syndrome with microdeletions including only the PITX2 and ENPEP (glutamyl aminopeptidase) genes. PMID: 17850355
  7. Data show that APA appears to be an essential enzyme in the control of blood pressure via degradation of Angiotensin II. PMID: 17990103
  8. Data show that aminopeptidase A (APA) plays important roles in the regulation of blood pressure under both the physiological and pathological conditions. PMID: 17999179
  9. Data show that brain aminopeptidases act upon Ang I, Ang II and Ang III in order to activate brain AT(1) receptors. PMID: 18188697
  10. rare and common variation in ENPEP may contribute to the development of renal and hypertensive disorders and warrants further study PMID: 18206321
  11. The presence of APA in several human brain nuclei sensitive to angiotensins and involved in blood pressure regulation suggests that APA in humans is an integral component of the brain renin angiotensin system. PMID: 18410507
  12. A novel nonsense polymorphism in the aminopeptidase-a gene was associated with hypertension among postmenopausal women, and those with marginal calcium and vitamin D intake. PMID: 18550936
  13. Angiotensin III and aminopeptidase A and N, related converting enzymes, contribute to cell proliferation of prostate cancer and may be implicated in cancer progression. PMID: 18677709
  14. Over-expressed APA drastically reduces, in a calcium dependent manner, full-length-Abeta but not amyloid-beta precursor protein (APP) intracellular domain in a cell-free model of Abeta production. PMID: 19187443
  15. The activity of glutamyl aminopeptidase was significally increased in head and neck squamous cell carcinoma tissue. PMID: 19373777

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Protein Families
Peptidase M1 family
Tissue Specificity
Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Database Links

HGNC: 3355

OMIM: 138297

KEGG: hsa:2028

STRING: 9606.ENSP00000265162

UniGene: Hs.435765

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