Product Details

Uniprot No.

Target Names

Alternative Names

Glutamyl aminopeptidase (EAP) (EC 3.4.11.7) (Aminopeptidase A) (AP-A) (Differentiation antigen gp160) (CD antigen CD249), ENPEP

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Aminopeptidase A / CD249

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Glutamyl aminopeptidase, also known as aminopeptidase A or CD249, plays a critical role in the renin-angiotensin system by cleaving angiotensin II to generate angiotensin III, positioning it as a key regulator of blood pressure and fluid homeostasis. Beyond its enzymatic function, ENPEP serves as a differentiation antigen (gp160) expressed on vascular endothelial cells and renal proximal tubule epithelium, making it a valuable marker for studies in immunology and stem cell biology where lineage-specific identification is essential.

This recombinant monoclonal antibody, clone 2G11, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures experimental comparability across collaborating laboratories. The rabbit host origin provides high affinity binding characteristics, while affinity chromatography purification delivers a preparation optimized for sensitive detection applications.

Validation in immunohistochemistry demonstrates reliable performance in paraffin-embedded human kidney tissue, where ENPEP is abundantly expressed. Using a citrate buffer antigen retrieval protocol at pH 6.0 and working dilutions between 1:50 and 1:200, the antibody produces clear visualization of target protein distribution when detected with HRP-conjugated secondary systems and DAB chromogen. This validated IHC protocol, along with confirmed ELISA compatibility, provides researchers with flexibility across experimental platforms.

For investigators exploring renal physiology, cardiovascular regulation, or stem cell differentiation pathways, this antibody delivers the technical reliability needed to confidently characterize ENPEP expression in human tissue specimens.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA552900A0HU
Size	US$210
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Image	IHC image of CSB-RA552900A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ENPEP Recombinant Monoclonal Antibody

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