FEN1 Antibody

Datasheet
Code CSB-RA008585A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
    All lanes: FEN1 antibody at 0.775μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 43, 36 KDa
    Observed band size: 43 KDa

  • IHC image of CSB-RA008585A0HU diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-RA008585A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Uniprot No. P39748
Target Names FEN1
Alternative Names DNase IV antibody; FEN-1 antibody; FEN1 antibody; FEN1_HUMAN antibody; Flap endonuclease 1 antibody; Flap structure specific endonuclease 1 antibody; Flap structure-specific endonuclease 1 antibody; hFEN-1 antibody; hFEN1 antibody; Maturation factor 1 antibody; MF1 antibody; Rad2 antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human FEN1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Gene References into Functions
  1. Results provide evidence that FEN1 rs174538 and rs4246215 are significantly associated with Wilms tumor susceptibility. PMID: 29937070
  2. FEN1 was an independent predictor of survival in pancreatic ductal adenocarcinoma PMID: 29735403
  3. The FEN1 rs174538 A allele is a novel protective biomarker for endometriosis PMID: 29109095
  4. This study revealed that cell apoptosis dysfunction plays a key role in the pathogenesis of LN, even though the difference in FEN1 gene 61563142-61563342 between LN patients and healthy individuals was not statistically significant. PMID: 29239254
  5. Results suggest that FEN1 polymorphisms may reduce the risk of breast cancer in Chinese women. PMID: 27801669
  6. FEN1 is essential for prolifera- tion and cisplatin resistance of lung cancer cells. PMID: 28371273
  7. Our results suggest that functional polymorphism FEN1 rs174538 G>A might affect personal susceptibility to esophageal squamous cell carcinoma . This result provides a solid theoretical foundation for further clinical study using larger sample sizes. PMID: 28319330
  8. FEN1 sculpts DNA with diffusion-limited kinetics to test DNA substrate. This DNA distortion mutually 'locks' protein and DNA conformation and enables substrate verification with extreme precision. PMID: 28230529
  9. Notably, non-small cell lung cancer patients with FEN1-overexpressed cancers were prone to have poor differentiation and poor prognosis. Furthermore, knockdown of FEN1 resulted in G1/S or G2/M phase cell cycle arrest and suppressed in vitro cellular proliferation in NSCLC cancer cells. PMID: 29037854
  10. Data indicate that human cancer-associated genetic alterations in the FEN1 gene can contribute substantially to cancer development. PMID: 27270424
  11. WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase delta progression across telomeric G-rich repeats, only WRN promotes sequential strand displacement synthesis and FEN1 cleavage. PMID: 27849570
  12. The FEN1 rs174538 A allele is a protective biomarker for childhood ALL and this association is more significant in males and in patients at onset age of 3.5 years or older. PMID: 26708601
  13. Data indicate that Flap endonuclease 1 (FEN1) single nucleotide polymorphisms and haplotypes are associated with gallbladder cancer risk. PMID: 26668074
  14. the aim of the present study was to determine the role of FEN1 in the chemosensitivity of SGC7901 cells. PMID: 26718738
  15. Binding of DNA to human FEN1 in a bent conformation occurred independently of 5'-flap accommodation and did not require active site metal ions or the presence of conserved active site residues. PMID: 26884332
  16. In this study, we found that cohesion establishment factors, like CHlR1, cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. PMID: 26032365
  17. Under DNA repair conditions, FEN1 efficiently repaired the 5'-phoshotyrosyl bond-containing SSB substrates in the presence of DNA ligase and DNA polymerase. PMID: 26581212
  18. WDR4 regulates FEN1's potential DNA cleavage threat near the site of replication. PMID: 26751069
  19. Patients with expression of both FEN1 and RAD54B were prone to have advanced nodal involvement and significantly poor prognosis. PMID: 26431531
  20. Fen1 significantly stimulated trinucleotide repeats expansion by Pol beta, but not by the related enzyme Pol lambda. PMID: 25687118
  21. YY1 is a transcription repressor of FEN1 regulating FEN1 levels in response to DNA damaging agents. PMID: 25885449
  22. EXO1 and FEN1 cleaved the substrate at the boundary between the single-stranded 5' flap and the duplex, whereas FAN1 incised it three to four nucleotides in the double-stranded region. PMID: 26221031
  23. Rate-determining step of FEN1 reflects a kinetic bias against long flaps and trinucleotide repeat sequences. PMID: 26160176
  24. FEN1 -69G>A and 4150G>T polymorphisms may be associated with cancer susceptibility in Chinese population. PMID: 25154853
  25. Because FEN1 mutations have been identified in human cancers, our findings raise the possibility that unresolved RNA:DNA hybrid structures contribute to the genomic instability associated with cancer PMID: 25922071
  26. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
  27. FEN1 protein expression was evaluated in 568 oestrogen receptor (ER) negative breast cancers, 894 ER positive breast cancers and 156 ovarian epithelial cancers. FEN1 is a promising biomarker in breast and ovarian epithelial cancer. PMID: 24880630
  28. The FEN1 E359K germline mutation disrupts the FEN1-WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers. PMID: 24608430
  29. the mutations of the FEN1 gene maybe a rare event in the development of hepatocellular carcinomas in Korean population PMID: 24164220
  30. FEN1 SNPs and haplotypes were significantly associated with breast cancer risk. The risk alleles showed decreased FEN1 mRNA expression in breast tissues. PMID: 24440783
  31. The homotrimeric proliferating cell nuclear antigen protein (PCNA) coordinates the actions of DNA polymerase, FEN1 and DNA ligase by facilitating the hand-off intermediates between each protein during Okazaki fragment maturation PMID: 22918592
  32. Conserved basic amino acids are not required for double nucleotide unpairing and appear to act cooperatively, whereas the helical cap plays an unexpected role in hFEN1-substrate rearrangement PMID: 24126913
  33. The data provides the first observations of unpaired conformations in DNA substrates bound to hFEN1 in the presence of catalytically inert active-site metal ions and define the role of amino acid residues and divalent metal ion cofactors in this process. PMID: 23975198
  34. A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties. PMID: 23675412
  35. This study suggested that FEN1 polymorphisms and haplotypes are associated with glioma risk. PMID: 23184144
  36. We identified small-molecule inhibitors of one such target, FEN1, and showed that these compounds were able to selectively kill human cells carrying cancer-relevant mutations. PMID: 23382697
  37. These studies demonstrate a novel role for APC in the suppression of Fen1 activity in the BER pathway PMID: 22787431
  38. Cell-cycle-dependent timing of FEN1 nuclease activity is essential for cell-cycle progression and the maintenance of genome stability. PMID: 22749529
  39. The crystal structure of the complex of importin alpha with a peptide corresponding to the FEN1 nuclear localization sequence was solved. PMID: 22751659
  40. Review: identifies superfamily motifs such as the helical gateway that select for ss-dsDNA junctions and provides key biological insights into FEN1 specificity and regulation. PMID: 22244820
  41. Data show models for the ternary PCNA/FEN1/DNA and Rad9-Rad1-Hus1 (9-1-1 complex)/FEN1/DNA assemblies. PMID: 22586102
  42. Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
  43. FEN1 as an important gene in human gastrointestinal oncogenesis and genetic polymorphisms in FEN1 confer susceptibility to gastrointestinal cancers. PMID: 22072618
  44. Activity of FEN1 endonuclease on nucleosome substrates is dependent upon DNA sequence but not flap orientation. PMID: 21454907
  45. Structural and functional analyses of human FEN1:DNA complexes show structure-specific, sequence-independent recognition for nicked dsDNA bent 100 degrees with unpaired 3' and 5' flaps. PMID: 21496641
  46. these results show that FEN1 and telomerase association occurs throughout the S phase, with the maximum association in the mid S phase. PMID: 21345332
  47. FEN1 10154T variant allele decreases arachidonic acid and arachidonic acid/linoleic acid in the serum phospholipids of carriers of the APOA5-1131C allele PMID: 20802161
  48. the methylated form, but not the phosphorylated form, of FEN1 strongly interacts with proliferating cell nuclear antigen (PCNA), ensuring the 'on' and 'off' timing of its reaction PMID: 20729856
  49. FEN1 and EXO1 can eliminate structures formed by trinucleotide repeats in the course of replication, relying on endonucleolytic and exonucleolytic activities, respectively. PMID: 20643645
  50. FEN1 maintains stable telomeres by facilitating replication through the G-rich lagging strand telomere, thereby ensuring high fidelity telomere replication. PMID: 20551483
  51. Herpes simplex virus 1 DNA polymerase stimulated the nick translation activity of human Fen-1 on DNA- or RNA-containing primer/templates, indicating that the activities were coordinated. PMID: 20444887
  52. Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates. PMID: 20019387
  53. results suggest that EXO1 may act at transcription-induced telomeric structures to promote telomere recombination while FEN1 has a dominant role in lagging strand replication at telomeres PMID: 20126648
  54. DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro. PMID: 11948189
  55. Arginine residues 47 and 70 are involved in DNA substrate interactions and cleavage site determination PMID: 11986308
  56. the rate of excision by FEN-1 of 5'-flaps from short- and long-flap oligonucleotide substrates that mimic pre- and post-repair HIV-1 integration intermediates, respectively, and the effect of HIV-1 integrase on these reactions were examined PMID: 12065902
  57. stimulated by APE1 for progression through the base excision repair pathway PMID: 12200445
  58. interaction of the WRN gene product with human 5' flap endonuclease/5'-3' exonuclease (FEN-1) PMID: 12356323
  59. flexible loop is important for efficient cleavage through positioning the 5' flap and the catalytic site PMID: 12411510
  60. Single nucleotide polymorphism in exon 15 of the XPG gene exhibited marginally significant increased frequency of the variant allele compared to controls. PMID: 12494477
  61. This protein can procss the 5'-flap DNA of CTG/CAG triplet repeat derived from human genetic diseases. PMID: 12515398
  62. human flap endonuclease 1 mutants employ endonuclease rather than exonuclease to prevent triplet repeat expansion PMID: 12554738
  63. C-terminal lysines are important for the endonuclease and exonuclease activities likely through DNA binding PMID: 12683998
  64. Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation. PMID: 12853968
  65. action at DNA replication forks PMID: 12856420
  66. We report here that faulty processing by FEN-1 initiates repeat instability in mammalian cells PMID: 12917330
  67. Alterations of FEN1 are not likely to contribute to development of lung cancer. PMID: 14562054
  68. WRN and FEN-1 proteins function together to process DNA structures associated with the replication fork. PMID: 14657243
  69. flap endonuclease-1 is stimulated by the Bloom's syndrome protein PMID: 14688284
  70. model for human FEN-1 protein interaction with its DNA substrates PMID: 15037610
  71. substrate specificity of Fen1 has been functionally conserved over a billion years from archaea to human PMID: 15131255
  72. identification of rad9-rad1-hus1 as a damage-specific activator of flap endonuclease 1 PMID: 15556996
  73. pol beta cooperates with FEN1 to remove DNA damage via a "Hit and Run" mechanism, involving alternating short gap production by FEN1 and gap filling by pol beta PMID: 15561706
  74. results suggest that BLM suppresses genome instability by aiding FEN1 cleavage of structure-containing flaps PMID: 15579905
  75. a novel gap endonuclease (GEN) activity of human flap endonuclease 1 (FEN-1), critical in resolving stalled replication fork PMID: 15592449
  76. The structural basis of the FEN1-PCNA interaction was revealed by the crystal structure of the complex between human FEN1 and proliferating cell nuclear antigen PMID: 15616578
  77. Fen1 exchange depends on the rate of movement of replication forks. PMID: 15758026
  78. A general DNA substrate-binding model that helps explains how the single active center of FEN-1 can perform cleavage on various substrates. PMID: 16582103
  79. The rearrangement frequency was found to increase with array size, and partial complementation of the rad27Delta mutation by hFEN1 demonstrated that the production of novel CEB1 alleles is Rad52 and Rad51 dependent. PMID: 16914748
  80. Both pol-beta and Fen-1 interact with a 138-amino-acid peptide from adenomatosis polyposis coli protein at the DNA repair inhibitory (DRI) domain. PMID: 17176113
  81. endogenously expressed FEN-1 associates with the native pS2 gene in MCF-7 cells and influences estrogen-responsive gene expression PMID: 17488975
  82. Asymmetric unit is predicted to contain two complexes in the crystallographic asymmetric unit. A diffraction data set was collected to a resolution of 2.75 A. PMID: 18097100
  83. C-terminus of FEN-1 is important for protein binding PMID: 18291413
  84. contributes to telomere stability by ensuring efficient telomere replication PMID: 18394896
  85. The replacement of Ser(187) by Ala, eliminating the only phosphorylation site, retains FEN1 in nucleoli. PMID: 18443037
  86. hChlR1 has a role in the establishment of sister chromatid cohesion, with Ctf18-RFC and Fen1 PMID: 18499658
  87. Repair of 2-deoxyribonolactone is via long-patch BER (LP-BER) dependent on flap endonuclease 1 (FEN1) in mitochondria. PMID: 18541666
  88. NEIL1 could also participate in strand displacement repair synthesis (long patch repair (LP-BER)) mediated by FEN-1 and stimulated by PCNA. PMID: 18662981
  89. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis. PMID: 18786645
  90. findings show that FEN1 is significantly up-regulated in multiple cancers and the aberrant expression of FEN1 is associated with hypomethylation of the CpG island within the FEN1 promoter in tumor cells PMID: 19010819
  91. FEN1 and telomerase physically co-exist as a complex and that FEN1 can regulate telomerase activity at telomeres in mammalian cells. PMID: 19068479
  92. Data show that replication factor C (RFC), via several stimulatory motifs per molecule, potently activates flap endonuclease 1 (FEN1), making RFC a critical partner with FEN1 for the processing of eukaryotic Okazaki fragments. PMID: 19208620
  93. RAD54B-deficient human colorectal cancer cells are sensitive to synthetic lethal killing by reduced FEN1 expression, while isogenic RAD54B proficient cells are not PMID: 19218431
  94. Abasic sites can also affect the strand displacement activity of the lagging strand DNA pol delta that can be overcome through a polymerase switch, involving the combined physical and functional interaction of DNA pol beta and Flap endonuclease 1. PMID: 19329428
  95. the presence of a 3'-flap is the critical feature for efficient hFEN1 substrate recognition and catalysis PMID: 19525235
  96. These results highlight FEN1 as an important gene in human carcinogenesis and genetic polymorphisms in FEN1 confer susceptibility to lung cancer. PMID: 19618370
  97. Data show that disruption of DNA polymerase beta and FEN1 coordination during long-patch base excision repair results in CAG repeat expansion. PMID: 19674974
  98. Direct physical interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. PMID: 11601988

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Subcellular Location Isoform 1: Nucleus, nucleolus, Nucleus, nucleoplasm
Protein Families XPG/RAD2 endonuclease family, FEN1 subfamily
Database Links

HGNC: 3650

OMIM: 600393

KEGG: hsa:2237

STRING: 9606.ENSP00000305480

UniGene: Hs.409065

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