Greater than 85% as determined by SDS-PAGE.
Epigenetics and Nuclear Signaling
DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2
Homo sapiens (Human)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The region for expressing recombinant Human FEN1 contains amino acids 1-380. This FEN1 protein is expected to have a theoretical molecular weight of 50.0 kDa. Expression of this FEN1 protein is conducted in e.coli. The FEN1 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant FEN1 protein during the following stages.
Flap endonuclease 1 (FEN1) is a multifunctional endonuclease involved in DNA replication and repair processes. In humans, FEN1 plays a crucial role in processing DNA intermediates during Okazaki fragment maturation, long-patch base excision repair, and nucleotide excision repair. As a structure-specific nuclease, FEN1 cleaves 5' flaps, flap structures that arise during DNA synthesis. This activity is vital for maintaining genomic stability and integrity. FEN1 also participates in DNA damage response pathways, contributing to the repair of various lesions. Dysregulation of FEN1 has been associated with genomic instability and is implicated in cancer development. Understanding FEN1's functions is essential for unraveling DNA repair mechanisms and potential therapeutic targets for cancer treatment.