Product Details

Uniprot No.

Target Names

Alternative Names

Serine/threonine-protein kinase PAK 2 (EC 2.7.11.1) (Gamma-PAK) (PAK65) (S6/H4 kinase) (p21-activated kinase 2) (PAK-2) (p58) [Cleaved into: PAK-2p27 (p27), PAK-2p34 (p34) (C-t-PAK2)], PAK2

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PAK2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PAK2, or p21-activated kinase 2, is a serine/threonine kinase that plays a central role in cytoskeletal dynamics, cell motility, and apoptotic signaling. As a downstream effector of Rho family GTPases, PAK2 integrates signals that regulate cell survival and death pathways, making it a compelling target in cancer biology, neuroscience, and signal transduction research. Notably, caspase-mediated cleavage of PAK2 during apoptosis generates constitutively active fragments (PAK-2p27 and PAK-2p34), adding another layer of functional complexity to its study.

This recombinant rabbit monoclonal antibody, clone 6D12, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing you to confidently compare results across extended studies without concerns about antibody variability.

Validation data demonstrates robust performance across multiple applications. In western blotting, the antibody detects a clean band at the predicted 59 kDa molecular weight in human cell lines including 293, Jurkat, and Raji, as well as in mouse and rat brain tissue, confirming reliable cross-species reactivity for comparative studies. Flow cytometry analysis in HepG2 cells shows clear separation from isotype control, supporting intracellular detection applications. Immunoprecipitation experiments in Raji lysates further validate the antibody's utility for protein interaction studies and enrichment workflows.

With confirmed performance in ELISA, western blot, flow cytometry, and immunoprecipitation, this antibody provides flexibility across experimental platforms investigating PAK2 function in neurological processes, tumor biology, and cellular signaling mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

Gene References into Functions

Overexpression of PAK2 in oral squamous cell carcinomas may be associated with an advanced pathology grade. PMID: 29714078
the promotion of apoptosis by human cytomegalovirusmiRUS45p in cells was specifically mediated via inhibition of PAK2 expression. PMID: 28765936
leukaemic cells explicitly require PAK2 to grow towards an extracellular matrix. PAK2-deficient cells fail to form colonies in methylcellulose and to induce lymphomas in vivo. PAK2 might therefore be the critical isoform in leukaemic cells by controlling tumour growth PMID: 28707321
Results show that PAK2 kinase plays an alternative anti-apoptotic role, phosphorylating caspase-7 and promoting unfettered cell growth and chemotherapeutic resistance. PMID: 27889207
We found that overexpression of miR-137 inhibited the proliferation of melanoma cells, which could be phenocopied by knockdown of PAK2 using siRNAs. PMID: 26186482
PAK2 is a direct effector of TSC1-TSC2-RHEB signaling and a new target for rational drug therapy in TSC. PMID: 26412398
Nef exploits PAK2 in a stepwise mechanism in which its kinase activity cooperates with an adaptor function for the exocyst complex to inhibit host cell actin dynamics. PMID: 26350970
Cytoplasmic Pak2 may promote cell proliferation in normal endometrium during menstrual cycle. PMID: 26218748
Further analyses show that HDAC6 may promote growth of GBM cells through inhibition of SMAD2 phosphorylation to downregulate p21 PMID: 26150340
Findings indicate that repression of microRNA miR-134 and consequent up-regulation of p21-activated kinase 2 (Pak2) might contribute to paclitaxel resistance. PMID: 26363097
Inhibition of PAK activation at late G2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry. PMID: 24840740
Results identified Pak2 as a possibly important mediator of ovarian cancer cell migration on extracellular matrix. PMID: 25050916
PAK2 activation may be associated with advanced tumor progression and poor prognosis of gastric cancer PMID: 24621074
Prostasin repress cancer cells and contribute to chemoresistance by modulating the CASP/PAK2-p34/actin pathway. PMID: 24434518
Authors demonstrate that HIV-1 Nef expression mediates phosphorylation of Mek1 on serine298 and Pak2 on serine192/197 in T cell lines as well as primary human T cells. PMID: 23746211
thrombin induces monocyte/macrophage migration via PAR1-Galpha12-dependent Pyk2-mediated Gab1 and p115 RhoGEF interactions, leading to Rac1- and RhoA-targeted Pak2 activation. PMID: 24025335
PAK2 negatively modulate TGF-beta signaling by attenuating the receptor-Smad interaction and thus Smad activation PMID: 22393057
Low-to-moderate penetrance protein coding mutations or non-coding mutations at DLG1 and/or PAK2, or a nearby gene, may reproduce the behavioral characteristics of the 3q29 microdeletion. PMID: 21850710
The ability of Nef to associate with PAK2 correlates with the ability to enhance HIV-1 replication. PMID: 21819585
highly expressed PAK2 mediates chemotherapeutic resistance in human breast invasive ductal carcinoma by negatively regulating caspase-7 activity PMID: 21555521
High PAK2 is associated with melanoma. PMID: 21177766
Mechanistic studies of the autoactivation of PAK2: a two-step model of cis initiation followed by trans amplification. PMID: 21098037
analysis of evolutionary conserved residues that are crucial for the catalytic activity of PKA and Pak2 PMID: 20209159
The association between the CD4 receptor and protein kinase pp58 and the protein-tyrosine kinase within the cell introduces a specific pathway by which T lymphocytes become activated. PMID: 20724730
MYO18A is a novel binding partner of the PAK2/betaPIX/GIT1 complex and suggest that MYO18A may play an important role in regulating epithelial cell migration via affecting multiple cell machineries. PMID: 19923322
Knockdown of PAK2 enhances loss of cell-cell junctions and increases lamellipodium extension but does not affect migration speed Hepatocyte Growth Factor (HGF) stimulated DU145 prostate carcinoma cells. PMID: 19628037
The enzymatic phosphorylation reaction of PAK2 can be best interpreted by a rapid-equilibrium random bi-bi reaction model; the catalysis reaction is partially limited by both the phosphoryl group transfer and the product release steps. PMID: 12549935
The opposing effects of Core protein on the transcription pf P21 might be important in the progression of liver disease in HCV-positive patients. PMID: 12823590
Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation PMID: 12853446
sites of PAK2 autophosphorylation in the regulatory and the catalytic domains and their kinetic effect; multiple regions of PAK2 are involved in the enzyme-substrate recognition PMID: 12907671
Pak2 phosphorylates Myc at three sites (T358, S373, and T400) and affects Myc functions both in vitro and in vivo PMID: 14749374
Nef induces signal transduction via the recruitment of a signaling machinery including Pak2 into lipid rafts, thereby mimicking a physiological cellular mechanism to initiate the TCR cascade. PMID: 15047825
PAK2 kinase activity is increased in response to TCR stimulation; results suggest a novel role for PAK2 as a positive regulator of T cell activation. PMID: 15187108
interacts with Nef proteins from SIV infected chimpanzees PMID: 15194762
Pak2 has a role in the down-regulation of translation initiation in apoptosis by phosphorylation of Mnk1 PMID: 15234964
PS-GAP is a novel regulator of caspase-activated PAK-2 PMID: 15471851
PAK-2 is activated in 1-LN prostate cancer cells by a proteinase inhibitor, alpha 2-macroglobulin PMID: 15908432
binding of Cdc42 localizes Pak2 to the endoplasmic reticulum, where autophosphorylation alters association of the two proteins PMID: 16204230
Pak2 binds to and phosphorylates initiation factor eIF4G, which inhibits association of eIF4E with m7GTP, reducing translation initiation. PMID: 16281055
Nef protein amino acids at positions 85, 89, 187, 188, and 191 (L, H, S, R, and F in the clade B consensus, respectively) are critical for Pak2 association and activation PMID: 16501114
Posttranslational myristoylation of PAK2 might be part of a unique series of mechanisms involved in the regulation of the later events of apoptosis. PMID: 16617111
c-Abl represents a target downstream of phosphatidylinositol 3-kinase-activated PAK2, which differentiates TGF-beta signaling in fibroblasts and epithelial cell lines. PMID: 16867995
This study of tissue-derived HIV-1 Nefs demonstrates that CD4 and MHC-I downregulation are highly conserved Nef functions, while Pak2 association is variable in late stage AIDS patients. PMID: 16979207
define a new class of PAK-interacting proteins, which play an important role in actin cytoskeletal reorganization PMID: 17543336
interaction of Nef with PAK2 does not play a major role in T-cell activation, viral replication, and apoptosis. PMID: 17881449
protein phosphatase 1alpha can act directly on phosphorylated Thr-402 in the activation loop of PAK2 and down-regulate its kinase activity PMID: 18176785
Data show RNAi-mediated or dominant-negative suppression of Pak2, major regulators of cytoskeletal signaling downstream of Cdc42 or Rac1, markedly inhibits EC lumen and tube formation. PMID: 18319301
Huntingtin exerts anti-apoptotic effects by binding to Pak2, which reduces the abilities of caspase-3 and caspase-8 to cleave Pak2 and convert it into a mediator of cell death. PMID: 19240112
PAK-2 activity controls the apoptotic response by regulating levels of activated caspase 3 and thereby its own cleavage to the proapoptotic PAK-2p34 fragment PMID: 19242610

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Subcellular Location

[Serine/threonine-protein kinase PAK 2]: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily

Tissue Specificity

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

Database Links

HGNC: 8591

OMIM: 605022

KEGG: hsa:5062

STRING: 9606.ENSP00000314067

UniGene: Hs.518530

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Code	CSB-RA592787A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue All lanes: PAK2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa Overlay histogram showing HepG2 cells stained with CSB-RA592787A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating PAK2 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA592787A0HU in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA592787A0HU(2µg)+ Raji whole cell lysate(500µg) Lane 3: Raji whole cell lysate (10µg)
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PAK2 Recombinant Monoclonal Antibody

Product Details

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Target Background