PAK2, also known as p21-activated kinase 2, is a member of the PAK family of serine/threonine kinases. It is also commonly referred to as gamma-PAK. PAK2 plays a crucial role in regulating cell morphology, motility, and survival. It is activated by small GTPases such as Rac and Cdc42 and participates in the MAPK and PI3K-Akt signaling pathways.
Dysregulation of PAK2 has been associated with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. In cancer, PAK2 promotes tumor cell invasion and metastasis. Several pharmaceutical companies are currently developing PAK2 inhibitors as potential anti-cancer therapies, aiming to block its activity and halt disease progression.
PAK2 Recombinant Monoclonal Antibody (CSB-RA592787A0HU)
Validated Data
Western Blot
Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue
All lanes: PAK2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Overlay histogram showing HepG2 cells stained with CSB-RA592787A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating PAK2 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA592787A0HU in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA592787A0HU(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)
The following PAK2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
PAK2 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA017406GA01HU | PAK2 Antibody | Human | ELISA,WB,IHC,IF |
CSB-PA622641DSR1HU | PAK2 Antibody | Human | ELISA, WB, IHC |
CSB-PA149930 | Phospho-PAK2 (Ser197) Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA101227 | PAK2 (Ab-192) Antibody | Human,Mouse | ELISA,WB |
CSB-PA827528 | PAK2 (Ab-197) Antibody | Human,Mouse | ELISA,WB |
CSB-PA951578 | Phospho-PAK2 (Ser141) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA020221 | Phospho-PAK2 (S192) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA020222 | Phospho-PAK2 (S141) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA020226 | Phospho-PAK2 (S197) Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA020229 | PAK2 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA020230 | PAK2 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA010536 | Phospho-PAK2 (S20) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA010537 | PAK2 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA922459 | PAK2 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA552213 | PAK2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-RA592787A0HU | PAK2 Recombinant Monoclonal Antibody | Human, Mouse, Rat | ELISA, WB, FC, IP |
PAK2 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP622641HU CSB-EP622641HU CSB-BP622641HU CSB-MP622641HU CSB-EP622641HU-B |
Recombinant Human Serine/threonine-protein kinase PAK 2 (PAK2) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PAK2 Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP641838RB CSB-EP641838RB CSB-BP641838RB CSB-MP641838RB CSB-EP641838RB-B |
Recombinant Rabbit Serine/threonine-protein kinase PAK 2 (PAK2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PAK2 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP727060RA CSB-EP727060RA CSB-BP727060RA CSB-MP727060RA CSB-EP727060RA-B |
Recombinant Rat Serine/threonine-protein kinase PAK 2 (Pak2) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PAK2 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP815882MO CSB-EP815882MO CSB-BP815882MO CSB-MP815882MO CSB-EP815882MO-B |
Recombinant Mouse Serine/threonine-protein kinase PAK 2 (Pak2) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |