PRKAR1A Antibody

Datasheet
Code CSB-RA018694A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: U87 whole cell lysate
    All lanes: PRKAR1A antibody at 1.1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 43, 39 KDa
    Observed band size: 43 KDa

  • Immunofluorescence staining of Hela cells with CSB-RA018694A0HU at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Product Details

Uniprot No. P10644
Target Names PRKAR1A
Alternative Names cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, PRKAR1A, PKR1, PRKAR1, TSE1
Species Reactivity Human
Immunogen A synthesized peptide derived from human PRKAR1A
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Involvement in disease Carney complex 1 (CNC1); Intracardiac myxoma (INTMYX); Primary pigmented nodular adrenocortical disease 1 (PPNAD1); Acrodysostosis 1, with or without hormone resistance (ACRDYS1)
Subcellular Location Cell membrane
Protein Families CAMP-dependent kinase regulatory chain family
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Database Links

HGNC: 9388

OMIM: 101800

KEGG: hsa:5573

STRING: 9606.ENSP00000351410

UniGene: Hs.280342

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