PRKAR1A Antibody

Code CSB-RA018694A0HU
Size US$350
Uniprot No. P10644
  • Western Blot
    Positive WB detected in: U87 whole cell lysate
    All lanes: PRKAR1A antibody at 1.1μg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 43, 39 KDa
    Observed band size: 43 KDa

  • Immunofluorescence staining of Hela cells with CSB-RA018694A0HU at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen A synthesized peptide derived from human PRKAR1A
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Relevance Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias cAMP-dependent protein kinase type I-alpha regulatory subunit, Tissue-specific extinguisher 1, TSE1, PRKAR1A, PKR1, PRKAR1, TSE1
Immunogen Species Human
Research Area Signal Transduction
Gene Names PRKAR1A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Involvement in disease Carney complex 1 (CNC1); Intracardiac myxoma (INTMYX); Primary pigmented nodular adrenocortical disease 1 (PPNAD1); Acrodysostosis 1, with or without hormone resistance (ACRDYS1)
Subcellular Location Cell membrane
Protein Families CAMP-dependent kinase regulatory chain family
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Database Links

HGNC: 9388

OMIM: 101800

KEGG: hsa:5573

STRING: 9606.ENSP00000351410

UniGene: Hs.280342

Pathway Insulin signaling pathway

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