Product Details

Uniprot No.

Target Names

Alternative Names

Prostaglandin G/H synthase 1 (EC 1.14.99.1) (Cyclooxygenase-1) (COX-1) (Prostaglandin H2 synthase 1) (PGH synthase 1) (PGHS-1) (PHS 1) (Prostaglandin-endoperoxide synthase 1), PTGS1, COX1

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human COX1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6B6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Prostaglandin G/H synthase 1, commonly known as cyclooxygenase-1 or COX-1, serves as a constitutively expressed enzyme that catalyzes the first committed step in prostaglandin biosynthesis. This housekeeping enzyme plays essential roles in maintaining gastrointestinal mucosal integrity, regulating platelet aggregation, and modulating renal blood flow. Understanding COX-1 expression and function has significant implications across inflammation research, cardiovascular biology, and cancer studies, particularly when investigating the balance between COX-1 and its inducible counterpart COX-2.

This recombinant monoclonal antibody, clone 6B6, offers the reproducibility and consistency that demanding research protocols require. Generated against a synthetic peptide derived from human COX-1 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The affinity-purified IgG format ensures minimal background in your experimental systems.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis at 1:2000 dilution reveals a clear band at 72 kDa in HL60 and SH-SY5Y whole cell lysates as well as mouse liver tissue, confirming cross-species reactivity between human and mouse samples. The observed molecular weight aligns with predicted sizes when accounting for post-translational modifications typical of this glycoprotein. Flow cytometry validation in HeLa cells shows distinct positive staining compared to isotype controls, with recommended dilutions ranging from 1:20 to 1:200 providing flexibility for optimization.

Whether investigating prostaglandin signaling pathways, examining inflammatory responses, or exploring metabolic regulation, this antibody delivers reliable detection across your experimental workflows.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

Gene References into Functions

COX1 inhibition significantly triggered cell death. PMID: 30320345
Prostaglandin D2 generation from human lung mast cells is catalysed exclusively by cyclooxygenase-1 PMID: 29225187
Extracellular histones disarrange vasoactive mediators release through a COX1-COX2-eNOS interaction in human endothelial cells. PMID: 28244682
Results suggested that in Chinese Han patients with stroke taking aspirin for secondary prevention, PTGS1 polymorphisms may increase the risk of poor functional outcomes and this effect may be modulated by the smoking status. PTGS1 gene-smoking interaction might in part reflect the heterogeneity in the prognosis of patients treated with aspirin. PMID: 28431615
analysis of co-existing variants in both F8 and PTGS-1 genes in a three-generation pedigree of hemophilia A; the PTGS-1 variant was associated with specific functional defects in the arachidonic acid pathway and more severe hemorrhage. PMID: 27629384
Data suggest expression of PTGS1 in colon is significantly correlated with expression of PTGS2, PTGES1, PTGER2, and PTGER4; this study was conducted in individuals at high risk for colon cancer treated with Mediterranean diet versus a Healthy Eating diet for prevention of colon cancer. (PG = prostaglandin; PTGS2 = PG-endoperoxide synthase 2; PTGES1 = PGE synthase protein; PTGER2 = PGE receptor 2; PTGER4 = PGE receptor 4) PMID: 27548026
Epithelial Cells Induce a Cyclo-Oxygenase-1-Dependent Endogenous Reduction in Airway Smooth Muscle Contractile Phenotype PMID: 28708434
Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
Panax quinquefolium saponin attenuated HUVEC apoptosis and improved the dual antiplatelet-mediated reduction of platelet adhesion to injured HUVECs and the underlying mechanisms may be associated with PI3K/AKT and COX pathways. PMID: 27401285
The interactions of COX-1of rs3842787 and cox-2 of rs20417 were associated with aspirin resistance of stroke. PMID: 27318652
Results show a trend toward an association between rs1236913 SNP and worst pain scores on nonsurgical root canal therapy post-treatment day 1 PMID: 26081267
We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
a new neutrophil-activating platelet-derived lipid generated by COX-1 is presented that can activate or prime human neutrophils, suggesting a role in innate immunity and acute inflammation. PMID: 27129261
Seminal COX-1 is over-expressed in infertile oligoasthenoteratozoospermic (OAT) men with varicocele (Vx) compared with fertile men with/without and infertile OAT men without Vx being associated with oxidative stress, Vx grade and Vx laterality. PMID: 25906828
variable turnover rate represents the most convincing determinant of the inter-individual variability in response to aspirin for primary prevention in diabetes mellitus PMID: 26245672
In Indian peptic ulcer hemorrhage patients, those with Cox-1 A842G polymorphisms tended to have less gastric ulcers among those with the A842G/C50T polymorphism. PMID: 27522738
single nucleotide polymorphisms show a protective effect under a dominant model of diisocyanate-induced asthma PMID: 25721048
inverse allosteric regulation likely underlies the ability of PGHS-2 to operate at low AA concentrations, when PGHS-1 is effectively latent. PMID: 26703471
PON1, P2Y12 and COX1 polymorphisms were associated with poorer vascular outcomes in patients with extracranial or intracranial stents. PMID: 26870959
The regulation of important oxylipin metabolic genes in peripheral blood mononuclear cells varied with the extent of change in arachidonic acid concentrations in the case of PTGS1 and ALOX12 regulation. PMID: 26672987
Immunohistochemical expression of COX-1 and VEGF is associated with poor prognostic parameters in renal cell carcinoma. PMID: 26339385
over-expression of COX-1 in an aberrant manner intersects multiple pro-tumorigenic pathways in high-grade serous ovarian cancer PMID: 25972361
COX-2 displays an enhanced facilitatory control on tachykininergic contractile activity in diverticular disease PMID: 24758697
Significant associations with NSAID-exacerbated respiratory disease were identified for: ALOX15 homozygous genotype and PTGS-1 rs5789 A/A homozygous genotype PMID: 26067486
Origin of the Enigmatic Stepwise Tight-Binding Inhibition of Cyclooxygenase-1. PMID: 26562384
cadmium at the highest tested concentrations modulates COX-1 and COX-2 at mRNA level in THP-1 macrophages PMID: 25645360
Aspirin Half Maximal Inhibitory Concentration Value on Platelet Cyclooxygenase1 in Severe Type-2 Diabetes Mellitus is not Significantly Different from that of Healthy Individuals. PMID: 23677911
Quantitative real-time PCR measurements of mRNA levels of genes PTGS1 and PTGS2 revealed no effect of the tested vanadium compound on the levels of analyzed transcripts. PMID: 25398544
Data suggest that, in organization of enzymes in post-translational endoplasmic reticulum, mPGES1 (microsomal prostaglandin E synthase-1) is likely co-localized with COX2 within a distance of 14.4 A; mPGES-1 is localized much farther from COX1. PMID: 25988363
Single nucleotide polymorphisms of RANK and PTGS1 show genetic associations with osteoproliferative changes in ankylosing spondylitis. PMID: 24651623
Studied the impact of blood type, functional polymorphism (T-1676C) of the COX-1 gene promoter, and clinical factors on the development of peptic ulcer during cardiovascular prophylaxis with low-dose aspirin. PMID: 25243161
Cigarette smoking aggravates COX-1-mediated platelet reactivity in young, otherwise healthy, smoking men. PMID: 23242413
Baseline omega-3 fatty acid levels do not influence TxA(2) generation in patients with or at high risk for CVD in the absence of COX-1 activity. PMID: 24370448
Data indicate that Protectin DX inhibited both cyclooxygenase-1 and -2 (COX-1 and COX-2) in neutrophils. PMID: 24254970
A report of allele frequencies on COX-1 polymorphisms in pediatric patients with cardiovascular anomalies. PMID: 24535852
Impacts of COX-1 gene polymorphisms on vascular outcomes in patients with ischemic stroke and treated with aspirin PMID: 24930730
The association of four common polymorphisms from four candidate genes (COX-1, COX-2, ITGA2B, ITGA2) with aspirin insensitivity PMID: 24244288
heterozygosity correlates with recurrent postoperative bleeding PMID: 24008976
There was no association between COX-1 C50T and COX-2 G765C polymorphisms and AR in Chinese stroke patients. In addition, COX-1 C50T and COX-2 G765C polymorphisms had no effect on the platelet response to aspirin PMID: 22972377
COX1 promoter polymorphisms (T-1676C and A-842G/ C50T) may not be associated with the risk of developing ulcerative colitis in a Japanese population. PMID: 18705313
as COX-1 and COX-2 express and affect VEGF synthesis in HNSCC cells, we should check COX-1 expression in investigations on cancer treatment by inhibiting COX-2-induced prostaglandins. PMID: 21301321
COX1 A-842G and C50T polymorphisms that contribute to resistance or augmented response to aspirin are rare in the Chinese population. PMID: 17559347
SNPs in COX1 -842A>G determined thrombotic complications in myocardial infarction. PMID: 22940005
COX-1 expression is widely detected in the hippocampus but decreases sharply in Niemann-Pick type C (NPC) knock-out mice compared to NPC+/+ mice. PMID: 23660496
the present study did not confirm correlations between tissue mRNA levels and protein content of COX-1 and EGFR. PMID: 24171795
rs2071746 in HO-1 gene, rs1330344 in COX-1 gene contribute to resistance to aspirin. PMID: 22609818
In PTGS1, a functional polymorphism was associated with increased rectal cancer risk. PMID: 24022467
Thromboxane A(2) generation, in the absence of platelet COX-1 activity, in patients with and without atherothrombotic myocardial infarction PMID: 23985963
COX1 inhibition by aspirin is suboptimal in 15% of patients with systemic lupus erythematosus. The suboptimal response was associated with metabolic syndrome, obesity, and higher CRP concentrations. PMID: 24022862
Overexpression of cyclooxygenase-1 correlates with renal cell carcinoma. PMID: 23886173

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Subcellular Location

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Protein Families

Prostaglandin G/H synthase family

Database Links

HGNC: 9604

OMIM: 176805

KEGG: hsa:5742

STRING: 9606.ENSP00000354612

UniGene: Hs.201978

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Code	CSB-RA918236A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: PTGS1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69, 65, 62, 57, 72, 73 kDa Observed band size: 72 kDa Overlay histogram showing Hela cells stained with CSB-RA918236A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PTGS1 Recombinant Monoclonal Antibody

Product Details

Citations

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Target Background