Code | CSB-RA202969A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
To develop the SOX9 recombinant monoclonal antibody, a rigorous and intricate process is required. Initially, the SOX9 monoclonal antibody is harvested and its gene sequence is determined. A vector containing the SOX9 monoclonal antibody gene is then generated and transfected into a host cell line for culturing. The SOX9 monoclonal antibody is produced using a synthetic peptide from human SOX9 as an immunogen. Subsequently, the SOX9 recombinant monoclonal antibody undergoes purification via affinity chromatography to ensure high specificity. This antibody is then thoroughly tested for its specificity in recognizing its target by utilizing ELISA and IHC assays. It only reacts with human SOX9 protein.
SOX9 is a critical transcription factor that regulates the development and differentiation of various tissues during embryonic development and adult tissue homeostasis. SOX9 is a master regulator of chondrogenesis. It is required for the differentiation of mesenchymal stem cells into chondrocytes, the cells that produce and maintain the cartilage matrix. SOX9 plays a critical role in male sex determination by regulating the differentiation of the gonads into testes during embryonic development. Its dysregulation can contribute to various diseases, such as skeletal dysplasias, osteoarthritis, and cancer.
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