Recombinant Mouse Gap junction delta-2 protein(Gjd2)

Code CSB-CF009461MO
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Gjd2
Uniprot No. O54851
Alternative Names Gjd2; Gja9; Gap junction delta-2 protein; Connexin-36; Cx36; Gap junction alpha-9 protein
Species Mus musculus (Mouse)
Expression Region 1-321
Target Protein Sequence MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQP GCNQACYDRAFPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQRERRYSTVFLALDRDP AESIGGPGGTGGGGSGGSKREDKKLQNAIVNGVLQNTETTSKETEPDCLEVKELTPHPSG LRTAARSKLRRQEGISRFYIIQVVFRNALEIGFLVGQYFLYGFSVPGLYECNRYPCIKEV ECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKLAVRGAQAKRKSVYEIRNK DLPRVSVPNFGRTQSSDSAYV
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Gene References into Functions
  1. data suggest a possible contribution of Cx36 to ALS pathogenesis amyotrophic lateral sclerosis PMID: 29246791
  2. Results indicate that even though Cx36 may be required for electrical coupling of suprachiasmatic nucleus cells, it does not affect coupling of molecular clock gene rhythms. Thus, electrical coupling of neurons and coupling of circadian clock gene oscillations can be regulated independently in the suprachiasmatic nucleus. PMID: 28576728
  3. In mice, strain differences were found, such that punctate labelling for Cx36 was differentially distributed in the molecular layer of C57BL/6 vs. CD1 mice. Cx36-puncta were localized to processes of stellate cells and other cerebellar interneurons. The vast majority of Cx36-puncta co-localized with Cx45-puncta, which in turn was associated with the scaffolding protein zonula occludens-1. PMID: 28561933
  4. Cx36 deletion therefore disrupts normal regulation of sympathetic outflow with effects on cardiovascular parameters. PMID: 28533325
  5. Deletion of connexin 36 increased the frequency and shifted the amplitude distributions of miniature inhibitory post synaptic potentials. PMID: 26864476
  6. retinal aberrant activity was reduced in the rd10/Cx36KO mice compared to rd10 controls, a direct evidence for involvement of Cx36-containing gap junctions in generating aberrant activity in RD. PMID: 26005040
  7. The results suggest that electrical synapses formed by Cx36-containing gap junctions occur in most of the widely distributed centers of the auditory system. PMID: 26188286
  8. The resilts of this study showed that the phosphorylation state of Cx36 as a key biochemical step at the crossroad of light-dark and circadian regulatory pathways that control photoreceptor coupling. PMID: 26241696
  9. The magnitudes of the 2f1-f2 distortion products were not different between Cx36 knockout and wild-type mice, suggesting similar cochlear function in the two groups PMID: 25891643
  10. Mg(2+)-dependent synaptic plasticity of Cx36-containing electrical synapses could underlie neuronal circuit reconfiguration via changes in brain energy metabolism that affects neuronal levels of intracellular ATP and [Mg(2+)]i. PMID: 25135336
  11. Gene expression data indicate that EPCs, or their soluble products, modulate the expression of the beta-cell surface molecule connexin 36 and affect glucose-stimulated insulin release in vitro. PMID: 24069942
  12. Some neurons exhibit at least two discriminatory mechanisms for assembling Cx36 into gap junctions. PMID: 24463820
  13. The Cx36 was expressed in the mouse carotid body and in the intestine at ileum and colon level. PMID: 22897942
  14. The results indicate the presence of Cx36-containing gap junctions in the sexually dimorphic dorsomedial nucleus and dorsolateral nucleus of both male and female rodents PMID: 24304165
  15. The genetic deletion of the GJ subunit Cx36 increased cell survivability by approximately 50% under excitotoxic condition. PMID: 25100592
  16. Data (including data from studies in knockout mice) suggest that Cx36/Gjd2 gap junction channels are necessary for full up-regulation of insulin secretion by islet cells; mice with defective Cx36/Gjd2 expression exhibit glucose intolerance. PMID: 24735890
  17. knockout of Cx36 in mice with ATP-insensitive KATP channels restores glucose homeostasis and normal insulin secretion. PMID: 24458355
  18. Because Cx36 expression and function is reduced in type 2 diabetes, the resulting defect in intraislet blood cell flow regulation may also play a significant role in diabetic pathology. PMID: 24326425
  19. This study demonistrated that regulation of interneuron excitability by gap junction which containing Gjd2 coupling with principal cells in mice. PMID: 24185427
  20. disruption of connexin36-mediated rod-cone coupling does not stop, delay or spatially restrict secondary cone degeneration and suggest that the gap junction-mediated bystander effect does not contribute to the progression of RP. PMID: 23468924
  21. Deletion of connexin 36 is neuroprotective, resulting in a better functional outcome and decreased infarct size after cerebral ischemia. PMID: 22960118
  22. The intercellular synchronization of Ca2+ oscillations evaluates Cx36-dependent coupling. PMID: 22848521
  23. These results indicate that gap junctions formed by Cx36 in spinal cord are required for maintenance of presynaptic inhibition, including the regulation of transmission from Ia muscle spindle afferents. PMID: 22615430
  24. the differences in Purkinje cell complex spike activity between wild type and cx36 knockouts likely reflect the loss of electrical coupling of inferior olive cells PMID: 17853112
  25. Results provide a context in which to examine functional relationships between these proteins at Cx36-containing electrical synapses in brain. PMID: 22722764
  26. The data identify Cx36 as a novel early marker of beta cells and as a target of Beta2/NeuroD1, which is essential for beta-cell development and differentiation. PMID: 22729650
  27. It was concluded that connexin-36 gap junctions regulate the in vivo dynamics of insulin secretion, which is important for glucose homeostasis. Coordinated pulsatility of individual islets enhanced first-phase elevation and second-phase pulses of insulin. PMID: 22511206
  28. Data suggest that gap junction coupling of beta-cells mediated by Cx36 is impaired in prediabetic mice (induced along with obesity by high-fat diet); prediabetic mice exhibit decrease in islet Cx36 and glucose-stimulated insulin secretion. PMID: 22569071
  29. Connexin 36 is expressed in beta and connexins 26 and 32 in acinar cells at the end of the secondary transition of mouse pancreatic development and increase during fetal and perinatal life. PMID: 22505190
  30. during development, acute activation of group II mGluRs causes up-regulation of Cx36 via post-transcriptional mechanisms PMID: 22342304
  31. Electrical transmission is supported by connexin36 (Cx36)-containing gap junctions at somato-somatic contacts of MesV neurons. PMID: 22457486
  32. both AF6 and MUPP1 are associated with Cx36-containing interneuronal gap junctions that form electrical synapses in adult rodent brain PMID: 22211808
  33. Cx36 deficiency in the mouse leads to reduced CaMKII levels in the striatum and behavioral changes in open-field activity, anxiety-related behavior in the light-dark box and one-trial object-place recognition. PMID: 21889545
  34. Cx36 decreases apoptosis & protects mouse beta cells against both cytotoxic drugs & cytokines in the islet environment at the onset of type 1 diabetes. Proinflammatory cytokines decreased its expression. PMID: 22056383
  35. We characterized connexin36 (Cx36)-expressing neurons of the adult rat somatosensory cortex PMID: 21467210
  36. Data suggest that learning and memory deficiencies in Cx36 knockout mice are due to deficiencies in LTP mechanisms. PMID: 21798314
  37. elimination of electrical synapses formed by connexin36 altered inhibitory efficacy and caused frequency facilitation of inhibition consistent with a decreased GABA release in the inhibitory network PMID: 21804029
  38. findings demonstrate the behavioral relevance of Cx36 gap junction-mediated electrical coupling between GABA neurons in mature animal PMID: 21638336
  39. The cx36 strongly modulate the firing pattern of individual RGCs, ensuring strongly correlated firing between nearby RGCs and normal patterning of retinogeniculate projections. PMID: 21734291
  40. in the 4-aminopyridine model of epilepsy in vitro, connexin36 is not critical for the generation of epileptiform discharges in GABAergic networks PMID: 21300748
  41. Results identify a motif within connexin 36 that may interact with other trafficking or scaffold proteins and thereby be responsible for its incorporation into electrical synapses. PMID: 20510366
  42. Cx36 is the predominant subunit of gap junctions in the proximal mouse retina, expressed by most ganglion cell subtypes, and thereby likely plays a major role in the concerted activity generated by electrical synapses PMID: 20058323
  43. Cx36 plays a role in the generation of certain forms of network synchronisation in the hippocampus PMID: 12042356
  44. Results indicate that connexins 26 and 36 are not present in mouse horizontal cells and therefore cannot contribute to feedback inhibition or horizontal cell coupling. PMID: 12064619
  45. spatial and temporal expression of connexin36 protein in neurons of the central nervous system PMID: 12107497
  46. Expression of mRNA encoding cx36 is observed in the olfactory epithelium mainly in ventral and lateral regions of the turbinates. PMID: 12687708
  47. connexin 36 expression is regulated by the transcriptional repressor NRSF/REST PMID: 14565956
  48. There is a molecular link between Cx36 and the stimulus-secretion pathway leading to insulin secretion. PMID: 14681053
  49. Connexin36 is localized to beta cells of pancreatic islets. PMID: 14980497
  50. Adequate but not excessive levels of Cx36 allowed for recover of normal insulin secretion in response to various secretagogues. PMID: 15023528

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Subcellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Protein Families Connexin family, Delta-type subfamily
Tissue Specificity Highly expressed in neurons.
Database Links

KEGG: mmu:14617

STRING: 10090.ENSMUSP00000087742

UniGene: Mm.389394

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