Recombinant Mouse Interleukin-4 receptor subunit alpha(Il4r)

Code CSB-CF011661MO
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Il4r
Uniprot No. P16382
Alternative Names Il4r; Il4raInterleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD antigen CD124) [Cleaved into: Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP)]
Species Mus musculus (Mouse)
Expression Region 26-810
Target Protein Sequence IKVLGEPTCFSDYIRTSTCEWFLDSAVDCSSQLCLHYRLMFFEFSENLTCIPRNSASTVCVCHMEMNRPVQSDRYQMELWAEHRQLWQGSFSPSGNVKPLAPDNLTLHTNVSDEWLLTWNNLYPSNNLLYKDLISMVNISREDNPAEFIVYNVTYKEPRLSFPINILMSGVYYTARVRVRSQILTGTWSEWSPSITWYNHFQLPLIQRLPLGVTISCLCIPLFCLFCYFSITKIKKIWWDQIPTPARSPLVAIIIQDAQVPLWDKQTRSQESTKYPHWKTCLDKLLPCLLKHRVKKKTDFPKAAPTKSLQSPGKAGWCPMEVSRTVLWPENVSVSVVRCMELFEAPVQNVEEEEDEIVKEDLSMSPENSGGCGFQESQADIMARLTENLFSDLLEAENGGLGQSALAESCSPLPSGSGQASVSWACLPMGPSEEATCQVTEQPSHPGPLSGSPAQSAPTLACTQVPLVLADNPAYRSFSDCCSPAPNPGELAPEQQQADHLEEEEPPSPADPHSSGPPMQPVESWEQILHMSVLQHGAAAGSTPAPAGGYQEFVQAVKQGAAQDPGVPGVRPSGDPGYKAFSSLLSSNGIRGDTAAAGTDDGHGGYKPFQNPVPNQSPSSVPLFTFGLDTELSPSPLNSDPPKSPPECLGLELGLKGGDWVKAPPPADQVPKPFGDDLGFGIVYSSLTCHLCGHLKQHHSQEEGGQSPIVASPGCGCCYDDRSPSLGSLSGALESCPEGIPPEANLMSAPKTPSNLSGEGKGPGHSPVPSQTTEVPVGALGIAVS
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Gene References into Functions
  1. The myeloid mineralocorticoid receptor controls inflammatory and fibrotic responses after renal injury via macrophage interleukin-4 receptor signaling. PMID: 29548765
  2. IL-4Ralpha signaling on dendritic cells promotes allergen-induced Th2 responses and eosinophil infiltration into the lung. PMID: 29343807
  3. blockade of IL-4Ralpha signaling has an anti-inflammatory and anti-fibrotic effect during toxin-induced liver fibrosis progression and retards - but does not prevent - fibrosis resolution. PMID: 29463471
  4. IL-4Ralpha deficiency inhibited the activation of STAT6 in the mouse kidneys in two experimental models of renal fibrosis. . In vitro, IL-4 activated STAT6 and stimulated expression of alpha-smooth muscle actin and fibronectin in mouse bone marrow monocytes. PMID: 28739140
  5. IL-13 is able to signal independent of the IL-4Ra chain in AD(atopic dermatitis), which may lead to the identification of molecular pathways downstream of IL-13 signaling that could be targeted in future therapies for AD. PMID: 26896776
  6. the IL-4/IL-13 Heteroreceptor and its cytokines IL-4 and IL-13 play a role in Th17 to Th1 conversion as well as the differential sensitivity of the effectors to Treg suppression, leading to control of experimental allergic encephalomyelitis PMID: 28801358
  7. Data, including data from studies in transgenic/knockout mice, suggest expression of Gab1/Gab2 is up-regulated in activated macrophages in pulmonary fibrosis; both Gab1/Gab2 are recruited to Il4r, synergistically enhancing downstream signal amplification. (Gab1 = growth factor receptor bound protein 2-associated protein 1; Gab2 = growth factor receptor bound protein 2-associated protein 2; Il4r = interleukin-4 receptor) PMID: 28687632
  8. increased expression of IL-4Ralpha on neonatal CD11b(+) mDCs was responsible for Th2 - biased RSV immunopathophysiology PMID: 28389622
  9. these data demonstrate that type 2 IL-4R signaling can directly impair neutrophil recruitment during infection and inflammation PMID: 27438770
  10. IL-4Ralpha mediated signaling has two opposing functions during experimental schistosomiasis depending on the stage of advancement of the disease; interrupting IL-4Ralpha mediated signaling might be a viable therapeutic strategy to ameliorate liver fibroproliferative pathology in diseases like chronic schistosomiasis PMID: 28827803
  11. a severe asthma-associated polymorphism in the gene encoding the interleukin (IL)-4 receptor alpha chain (Il4ra(R576)) promotes conversion of induced Treg (iTreg) cells toward a T helper 17 (TH17) cell fate. This skewing is mediated by the recruitment by IL-4Ralpha(R576) of the growth-factor-receptor-bound protein 2 (GRB2) adaptor protein, which drives IL-17 expression by activating a pathway that involves ERK, IL-6 an... PMID: 27479084
  12. using mice deficient for IL-4Ralpha, findings show after repeated schistosome infection, levels of regulatory IL-10 in the skin were reduced, accompanied by increased numbers of MHC-IIhigh cells and CD4+ T cells in the skin; study highlights a mechanism through which IL-4Ralpha may regulate the immune system through the induction of IL-10 and regulation of Fas/FasL mediated cell death PMID: 27505056
  13. Together, this study demonstrates that sustained IL-4Ralpha mediated signaling is required for the maintenance of anti-nematode type 2 immune responses, describing a novel function for IL-4Ralpha that is distinct from its role in immune polarization. PMID: 28651009
  14. this study shows that IL-33R and IL-4Ralpha promote macrophage proliferation independently of each other, but both are essential for induction of alternative activation PMID: 27592711
  15. IL-4Ralpha expression on CD4+ T cells plays a major role in the pathogenesis of chronic asthma and the regulation of allergen-specific TH17 responses, whereas acute asthmatic pathology and TH2 responses developed in the absence of IL-4Ralpha-responsive CD4+ T cells and IL-4. PMID: 26688514
  16. Genome-wide expression of isolated Kupffer cells following pressure-controlled hemorrhage revealed a significant increase of gene expression of Cxcl10, Il4ra, Csf2rb2, Lcn2, and Gbp5, which might represent new biomarkers for early diagnosis of post-traumatic complications. PMID: 27120970
  17. Il4ra mRNA expression was significantly increased after stroke. PMID: 26732561
  18. IL-4Ralpha-macrophages do not play a central role in TB disease progression PMID: 25790379
  19. During skin repair in mice, IL-4Ralpha-dependent macrophage activation controlled collagen fibril assembly. This was important for effective repair while having adverse pro-fibrotic effects. PMID: 26474656
  20. signaling through IL-4Ralpha has little effect on regulating the peripheral pro-inflammatory cytokine profile in this EAE variant but has distinct effects on the determination of lesion topography. PMID: 25427822
  21. Immunostaining and semiquantitative analysis revealed increased presence of IL4ra in the pulmonary vasculature of patients with connective tissue disease-associated PAH and Schistosoma-PAH compared with normal control subjects. PMID: 26192556
  22. IL4R mediates enhanced glucose and glutamine metabolism in breast cancer cells. PMID: 25746764
  23. Selective augmentation of IL-4Ralpha signaling in Treg cells induced their reprogramming into Th2-like cells and disease susceptibility towards food allergy. PMID: 25769611
  24. Data show that fibrillin-1 (FBN1) modulates bone marrow mesenchymal stem cells (BMMSCs) lineage differentiation via IL4 receptor alpha/mTOR protein signaling. PMID: 25534817
  25. The results demonstrate that, despite residual IL-4Ralpha-independent alternative macrophage activation and ongoing T(h)2-dependent allergic inflammation, abrogation of IL-4Ralpha-dependent aaMph is sufficient to confer resistance in pulmonary cryptococcosis. PMID: 23532373
  26. A beneficial role of early IL-4Ralpha signaling is demonstrated in pulmonary cryptococcosis, which contrasts with the well-known IL-4Ralpha-mediated detrimental effects in the late phase. PMID: 24475277
  27. TLR dependent antigen processing by IL-4Ralpha-responsive B cells producing IL-13 contribute significantly to CD4 T cell-mediated protective immunity against N. brasiliensis infection. PMID: 24204255
  28. these data demonstrate that abrogation of IL-4Ralpha signaling on DCs is severely detrimental to the host, leading to rapid disease progression, and increased survival of parasites in infected DCs due to reduced killing effector functions. PMID: 24204259
  29. Loa loa exhibits a differential survival and development in different strains of cytokine or cytokine receptor gene knockout mice with IL-4R and IL-5 playing critical roles in the host resistance to L. loa infection. PMID: 22348321
  30. a novel IL-4Ralpha-independent mechanism of alternative macrophage activation that is antibody-dependent and which both mediates anti-helminth immunity and prevents tissue disruption caused by migrating larvae. PMID: 24244174
  31. Trichinella spiralis 53-kDa protein (rTsP53) alternatively activates peritoneal macrophages via STAT6 but not via IL-4Ralpha in vitro. PMID: 24534206
  32. High Il4ra expression is associated with glioma. PMID: 24030977
  33. These findings support a pathogenic role for IL-4Ralpha on Th cells following respiratory syncytial virus reinfection PMID: 23543769
  34. LysM(cre)IL-4Ralpha(-/lox) mice and littermate controls also developed similar responses in acute ovalbumin- and house dust mite-induced allergic airway disease PMID: 22552110
  35. neither IFN-gamma nor IL-4R-alpha is a key regulator of Myeloid-derived suppressor cells and targeting these molecules is unlikely to significantly alter MDSC accumulation or function. PMID: 22673957
  36. These results demonstrate that transfer of IL-4Ralpha + macrophages is sufficient to enhance TH2-driven, allergic inflammation. PMID: 22292924
  37. lack of expression on T helper cells confers resistance in allergic bronchopulmonary mycosis PMID: 22333910
  38. signaling through receptor is essential for alternative activation of macrophages but not for lung inflammation and eosinophilia in experimental airway inflammation PMID: 22014099
  39. The Th2 response can thus enhance the intracellular persistence of Mtb, in part by mediating the alternative activation of macrophages via the IL-4Ralpha signaling pathway. PMID: 21825018
  40. study presents data demonstrating Nippostrongylu brasiliensis-driven airway mucus production to be dependent on smooth muscle cell IL-4Ralpha responsiveness PMID: 20737001
  41. observations demonstrate that IL-4Ralpha promotes airway hyperresponsiveness through multiple mechanisms and provide a model for testing smooth muscle-directed asthma therapeutics PMID: 21464224
  42. These data demonstrate that upon infection with Leishmania mexicana, initial lesion growth in BALB/c mice is dependent on non-T cell population(s) responsive to IL-4/IL-13 while progressive infection is dependent on CD4(+) T cells responsive to IL-4. PMID: 21245915
  43. Interleukin-4Ralpha is required for allergen-induced up-regulation of glutamic acid decarboxilase expression (GAD65 enzyme)in airway epithelium implicated in goblet cell metaplasia following acute house dust mite exposure. PMID: 20337645
  44. IL-4Ralpha responsiveness in airway smooth muscle cells during the early phase of allergic asthma is not, as suggested, necessary for the outcome of the disease. PMID: 20579713
  45. Control of RSV-induced lung injury by alternatively activated macrophages is IL-4R alpha-, TLR4-, and IFN-beta-dependent.( PMID: 20404812
  46. It was concluded that IL-4Ralpha responsiveness by smooth muscle cells and subsequent IL-4- and IL-13-mediated hypercontractility are required for host protection during acute schistosomiasis. PMID: 20360135
  47. These results point to a physiologic negative regulatory role for the Y709 ITIM in signaling through IL-4Ralpha, especially by IL-13 in the allergic response. PMID: 20392476
  48. downregulation of IL4Ra on highly activated Th cells was triggered by antigenic stimulation and rendered Th2 cells unresponsive to IL-4 PMID: 20018622
  49. the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Ralpha expression on CD4(+) T cells and macrophages/neutrophils. PMID: 19191917
  50. Results provide novel mechanistic insights in OSM's role in regulation of synergistic (with Il-4 or IL-13) eotaxin-1 responses and IL-4Ralpha expression in fibroblasts. PMID: 19799897

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Subcellular Location Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Secreted
Protein Families Type I cytokine receptor family, Type 4 subfamily
Tissue Specificity Expressed in both Th1 and Th2 cells.
Database Links

KEGG: mmu:16190

STRING: 10090.ENSMUSP00000033004

UniGene: Mm.233802

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