Recombinant Rat Gap junction delta-2 protein(Gjd2)

Code CSB-CF009461RA
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names Gjd2
Uniprot No. O70610
Alternative Names Gjd2; Gja9; Gap junction delta-2 protein; Connexin-36; Cx36; Gap junction alpha-9 protein
Species Rattus norvegicus (Rat)
Expression Region 1-321
Target Protein Sequence MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQP GCNQACYDRAFPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQRERRYSTVFLALDRDP AESIGGPGGTGGGGSGGSKREDKKLQNAIVNGVLQNTETTSKETEPDCLEVKELAPHPSG LRTAARSKLRRQEGISRFYIIQVVFRNALEIGFLVGQYFLYGFSVPGLYECNRYPCIKEV ECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKLAVRGAQAKRKSVYEIRNK DLPRVSVPNFGRTQSSDSAYV
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Gene References into Functions
  1. the protein expression levels of hippocampal Cx36 and Cx43 during epileptogenesis in the pilocarpine model of epilepsy, were examined. PMID: 28042145
  2. activation of neuronal nicotinic receptors is an early tool for protecting motoneurons from excitotoxicity and that this process is carried out via the combined decrease in Cx36 activity, overexpression of Hsp70 and fall in AIF translocation PMID: 28617431
  3. Cx45-puncta in the cerebellar molecular layer were co-localized with Cx36-puncta and additionally occurred along Bergmann glial processes adjacent to Cx43-puncta. Expression of both Cx43 and Cx45 in Bergmann glia of rat may contribute to the complex properties of junctional coupling between these cells and perhaps to their reported coupling with Purkinje cells. PMID: 28561933
  4. The results of the present study revealed that during memory consolidation in a passive avoidance paradigm, a rapid upregulation of Cx36 mRNA level occurs in the hippocampus, which declines to the control level by 24 h after training. PMID: 27913256
  5. Neuronal Cx36 plays a role in rhythmic firing in substantia gelatinosa of rat neonatal dorsal horn in vitro. PMID: 27462070
  6. The results suggest that electrical synapses formed by Cx36-containing gap junctions occur in most of the widely distributed centers of the auditory system. PMID: 26188286
  7. Cx36 expression in the rat inner plexiform layer displays a characteristic succession of changes during retinogenesis. PMID: 25110193
  8. The results indicate the presence of Cx36-containing gap junctions in the sexually dimorphic dorsomedial nucleus and dorsolateral nucleus of both male and female rodents PMID: 24304165
  9. Changes in connexins, including Cx36, Cx43, and Cx45, may contribute to the damage process. PMID: 24883012
  10. Data suggest that Cx36/Gjd2 gap junction channels are necessary for full up-regulation of insulin secretion by islet cells; glucose stimulation of insulin secretion requires closure of Cx36/Gjd2 hemichannels but gap junction channels remain open. PMID: 24735890
  11. Cx36 plays a central role in the oxidative stress and endoplasmic reticulum stress induced by cytokines and the subsequent regulation of AMPK activity. PMID: 24096873
  12. we used immunofluorescence localization of the major gap junction forming protein connexin36 to examine its possible association with hippocampal pyramidal cells PMID: 22771400
  13. total sleep deprivation significantly decreases the levels of connexin36 in the hypothalamus and this decrease maintains after sleep recovery PMID: 22158029
  14. interneuron classes form connexin36-immunopositive dendrodendritic gap junctions. PMID: 17122364
  15. Astrocytic and neuronal differentiation during development are governed by mechanisms that include the differential expression of Cx36. PMID: 21408068
  16. Young and adult beta cells, which respond to glucose, expressed significantly higher levels of Cx36 (also known as Gjd2) than fetal and newborn beta cells, which respond poorly to the sugar. PMID: 20361177
  17. Cx36 expression at both mRNA and protein level was upregulated during acquisition of focal seizures but returned to basal level after acquisition of secondarily-generalized seizures. PMID: 20153799
  18. Here we provide evidence against this hypothesis and demonstrate a direct relationship between Cx36 phosphorylation and AII amacrine cell coupling strength. PMID: 19940186
  19. data provide evidence that connexin 36-dependent signaling contributes to regulate the function of native and tumoral insulin-producing cells PMID: 12064624
  20. Connexin-36 contributes to control function of insulin-producing cells. PMID: 12766175
  21. connexin 36 expression is regulated by the transcriptional repressor NRSF/REST PMID: 14565956
  22. Cx36 mRNAs are localized in neuronal cells in both developing and adult rat brain. PMID: 14622215
  23. Affinity-purified antibody, recognizing single 36-kDa protein, labeled discrete puncta of subcellular structures associated with gap junctions in inner plexiform layer, also cytoplasm within somata and dendrites of retinal amacrine and ganglion cells. PMID: 15011262
  24. Immunogold double labeling reveals NMDA glutamate receptor 1 (NR1)-containing postsynaptic densities localized near Cx36-labeled gap junction in rat inferior olive. PMID: 15173637
  25. These results suggest that changes in neuronal gap junction expression may be one mechanism by which cocaine self-administration produces enduring changes in behavior. PMID: 16138316
  26. These data provide evidence that glucose represses the expression of Cx36 through the cAMP-PKA pathway, which activates a member of the CRE binding protein family. PMID: 16263767
  27. Quinidine and mefloquine reflects an involvement of neuronal Cx36 channels in SD generation/propagation, these results bear potential drug-discovery relevance for the migraine with aura. PMID: 17113924
  28. In both developing and adult locus coeruleus, By freeze-fracture replica immunogold labeling, Cx36 was found in ultrastructurally-defined neuronal gap junctions. PMID: 17601673
  29. during depolarization neurons release ATP by way of connexin 36 hemichannels PMID: 18211823
  30. The presence of Cx36 in ChAT-ir neurons is consistent with electrical coupling between sympathetic preganglionic motoneurons and between somatic motoneurons through gap junctions in the adult spinal cord. PMID: 18280223
  31. We found distinct BDV-dependent reductions in Cx36 mRNA and protein in the rat hippocampal formation and cerebellar cortex, suggesting altered neuronal network properties to be an important feature of persistent viral brain infections. PMID: 19038327

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Subcellular Location Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Protein Families Connexin family, Delta-type subfamily
Tissue Specificity Highly expressed in neurons.
Database Links

KEGG: rno:50564

STRING: 10116.ENSRNOP00000011078

UniGene: Rn.9904

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