Recombinant Human Interferon lambda-1 protein(IFNL1) (Active)

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Code CSB-AP002811HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 5 ng/ml, corresponding to a specific activity of >2.0x105 IU/mg.
Target Names IFNL1
Uniprot No. Q8IU54
Research Area Immunology
Alternative Names IFN-lambda-1, Interleukin-29, IL-29
Species Homo sapiens (Human)
Source E.coli
Expression Region 20-200aa
Complete Sequence GPVPTSKPTT TGKGCHIGRF KSLSPQELAS FKKARDALEE SLKLKNWSCS SPVFPGNWDL RLLQVRERPV ALEAELALTL KVLEAAAGPA LEDVLDQPLH TLHHILSQLQ ACIQPQPTAG PRPRGRLHHW LHRLQEAPKK ESAGCLEASV TFNLFRLLTR DLKYVADGNL CLRTSTHPES T
Mol. Weight 19.8 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets
Gene References into Functions
  1. SOCS2 impairs IFN/JAK/STAT signaling through reducing the stability of tyrosine kinase 2 (TYK2), downregulating the expression of type I and III IFN receptors, attenuating the phosphorylation and nucleus translocation of STAT1. PMID: 28496097
  2. In conclusion, IL-29 enhanced CXCL10 production in human oral epithelial cells via the p38 MAPK, STAT3, and NF-kappaB pathways, which might control Th1-cell accumulation in periodontal lesions and be involved in pathological processes in periodontal disease. PMID: 28753407
  3. The DNA binding domain of Ku70 was essential for formation of the Ku70-STING complex. Knocking down STING in primary human macrophages inhibited their ability to produce IFN-lambda1 in response to transfection with DNA or infection with the DNA virus HSV-2 (herpes simplex virus-2). Together, these data suggest that STING mediates the Ku70-mediated IFN-lambda1 innate immune response to exogenous DNA or DNA virus infection. PMID: 28720717
  4. The review focuses on the value of the type I and III interferon subtypes (alphas, beta and lambdas) as therapeutics for prevention and treatment of viral infections (influenza, herpes, human immunodeficiency virus and hepatitis viruses). PMID: 27544015
  5. Interferon lambda1/IL-29 and inorganic polyphosphate are novel regulators of neutrophil-driven thromboinflammation. PMID: 28678391
  6. IFN-lambda1 may play an important role in anti-respiratory syncytial virus infection. PMID: 28606236
  7. Ebola virus VP24, in addition of inhibiting IFN-induced antiviral responses, was found to efficiently inhibit type III IFN-lambda1 gene expression. PMID: 28595092
  8. STAT2 recruits USP18 to the type I IFN receptor subunit IFNAR2 via its constitutive membrane-distal STAT2-binding site. PMID: 28165510
  9. IFN-lambda1 is likely to play a role in the pathogenesis of CSU. Blocking IFN-lambda1 production may help to reduce the accumulation of inflammatory cells in the involved CSU skin. PMID: 27445435
  10. IL-29 stimulates inflammation and cartilage degradation by OA FLS, indicating that this cytokine is likely involved in the pathogenesis of OA. PMID: 27433031
  11. IL-29 is a potential biomarker for disease activity in anti-cyclic citrullinated peptide-antibodies positive rheumatoid arthritis patients. PMID: 28154345
  12. this study shows that serum levels of IL-29 play a role in the pathogenesis of Hashimoto's thyroiditis in Turkey PMID: 27617784
  13. serum levels of IL-32 and TNF-alpha may be diagnostic markers, and serum IL-29 levels may be associated with good prognosis in patients with gastric cancer PMID: 26219901
  14. Serum levels of IL-29 and IFN-gamma are predictive of relapse outcomes after hepatitis C treatment. PMID: 26342113
  15. IL-29 selectively induces CXCR3A-binding chemokines (CXCL9, CXCL10, CXCL11) in skin cells. Murine IL-29 counterpart induces skin T-cell infiltration and inflammation in mice. PMID: 26612594
  16. activation of TLR7 upregulated the expression levels of IFN-lambda1 and MMP-9 were increased by ~3 fold, whereas other genes (p53, PTEN, TIMP-1) were upregulated by ~2 fold, and VEGF was marginally upregulated after 10 min. PMID: 26718740
  17. IL-29 directly induces RANKL expression in rheumatoid arthritis-fibroblasts like synoviocytes via MAPK signaling pathway. PMID: 26420479
  18. showed that silencing IFN-lambda1 in T/T cell line reduced basal IFN-stimulated gene expression and improved antiviral activity PMID: 26896692
  19. IFN-lambda-1 and interferon-gamma levels in systemic sclerosis patients were significantly higher than those in healthy individuals. PMID: 26057401
  20. this study failed to prove any significant association between polymorphisms in IL29 gene rs30461 or IL10 gene (-1082, -819 and -592) or cytokine haplotype and either response to therapy or severity of HCV infection in children. PMID: 25936570
  21. IL-29 can aggravate LPS/TLR4-mediated inflammation. PMID: 26278073
  22. NS of severe fever with thrombocytopenia syndrome virus inhibited the activity of IFN-alpha1, IFN-beta, IFN-lambda1 and IFN-lambda2 through inhibition of STAT1 phosphorylation. PMID: 26353965
  23. the role of IFN-lambda in IDO regulation was investigated after influenza infection of respiratory epithelial cells. PMID: 25756191
  24. Data suggest that naturally occurring iDVGs (immunostimulatory defective viral genomes) trigger robust host antiviral/innate immunity responses including/requiring up-regulation of IFNL1 and IFNB1 (interferon beta 1) in respiratory mucosa. PMID: 26336095
  25. there seems to be an inverse link between IFN-lambda and the severity of allergic asthma and allergic asthma exacerbations. PMID: 25592858
  26. Studies indicate that the type III interferons (IFNs) or IFN-lambdas consists of four members: IFN-lambda1 (IL-29), IFN-lambda2 (IL28A), IFN-lambda3 (IL-28B) and IFN-lambda4. PMID: 26194286
  27. Increased quantity of IL-29 in GCF and plasma of subjects with periodontitis suggests a role in pathogenesis of periodontitis and the SNP (rs30461) is not related to susceptibility to periodontitis in this population of Indian individuals. PMID: 25255471
  28. Severity of rhinovirus-induced asthma symptoms is inversely related to resolution IFNL1 expression. PMID: 25784275
  29. IL-29 is dysregulated in patients with rheumatoid arthritis (RA), which may contribute to the RA pathogenesis via inducing the production of proinflammatory cytokines, chemokines or matrix metalloproteinases in synovial fibroblasts. PMID: 23078630
  30. intranasal delivery of adenovirus expressing protein suppresses allergic asthma in a murine model PMID: 24819718
  31. IFN-lambda1-mediated IL-12 production in macrophages induces IFN-gamma production in human NK cells. PMID: 25316442
  32. data suggest that the p38alpha MAPK pathway is linked with RLR signaling pathways and regulates the expression of early IFN genes after RNA stimulation cooperatively with IRF3 and NF-kappaB to induce antiviral responses further PMID: 25473098
  33. Interferon lambda 1 induces antiviral response to herpes simplex virus 1 infection. PMID: 25518713
  34. These results show that type III interferons (IFN-lambdas) play a critical protective role in human metapneumovirus infection. PMID: 25355870
  35. Results showed no association between genotypes and alleles of IL28A, IL28B or IL29 polymorphisms and Hepatitis C virus infection. PMID: 24269996
  36. This study is the first to show the activation of a type III interferon response in low-risk human papillomavirus positive cervical cells and suggests that the lack of this response may be related to lesion progression. PMID: 24510368
  37. our data suggest that human but not mouse hepatocytes are responsive to IFN-lambda in vivo. PMID: 24498220
  38. protein expression is inhibited by hepatitis C virus PMID: 23529855
  39. IFNL1 expression in the human colon is transcriptionally regulated by ZEB-1, BLIMP-1, and NF-kappaB p50 and p65. PMID: 24140069
  40. These data suggest that T(Helper)17 cell-derived IL-29, which is absent in atopic dermatitis, mediates the robust antiviral state on psoriatic skin, and demonstrate a new function of T(H)17 cells. PMID: 24068736
  41. HBV infection upregulates IL-27 expression, which, in turn, stimulates IFN-lambda1 production. PMID: 24337382
  42. Interferon regulatory factor (IRF)-3 and -7 are the key transcriptional factors for the induction of IL-28A and IL-28B genes, whereas NF-kappaB is an additional requirement for the induction of the IL-29 gene. PMID: 24385435
  43. IL-32 levels during viral infection mediate antiviral effects by stimulating the expression of IFN-lambda1. PMID: 23729669
  44. IFN-lambdas can also directly affect T cells through inhibition of the T helper 2 cell (Th2) responses. IFN-lambdas have varying immunomodulatory functions under different physiological conditions PMID: 23207147
  45. RA patients that presented knee joint involvement displayed higher serum IFN-lambda1 than patients without knee joint involvement, suggesting that abnormally elevated IFN-lambda1 levels in RA can associate with knee joint disease. PMID: 23039206
  46. The viral infection induced both IFN-lambda1 promoter activity and that of the 3'-untranslated region (UTR), indicating that IFN-lambda1 expression is also regulated at the post-transcriptional level. PMID: 23150165
  47. IL-29 can regulate expression of protease activated receptors and tryptase- and trypsin-induced IL-4 production in mast cells. PMID: 23218741
  48. These data support a model of coordinated cell- and ligand-specific expression of types I and III interferon. PMID: 22249201
  49. After non-surgical periodontal therapy, IL-29 levels increased both in chronic and aggressive periodontitis patients. PMID: 23151616
  50. IFN-alpha and IFN-lambda signal through distinct receptors, they induce expression of a common set of ISGs in hepatocytes. However, unlike IFN-alpha, IFN-lambda1 did not induce STAT activation or ISG expression by purified lymphocytes or monocytes PMID: 23258595
  51. Plasmacytoid dendritic cells are major producers of IFN-lambda1 (a type III interferon) in response to viral stimulation and also express functional receptors for this cytokine. PMID: 22891284
  52. Inactivated HHV-6B is a strong inducer of IFN-lambda1 in plasmacytoid dendritic cells and this induction is TLR9-dependent. PMID: 22701693
  53. modulates asthma exacerbations caused by human rhinoviruses PMID: 22135341
  54. IFN-lambda1 is probably involved in the renal disorder and arthritis progression of systemic lupus erythematosus and associated with disease activity. PMID: 21679442
  55. IL-29-induced TLR3 expression is involved in antiviral activity of IL-29 in KCs, which suggests a feasible method to cure certain viral infections of the skin PMID: 21847628
  56. MiR29 suppresses DNMT activity and thus induces expression of COX2 and PGE2 during influenza A virus infection. PMID: 22072783
  57. Hepatitis C virus infection is capable of inducing antiviral cytokines and pathways in primary liver cell cultures; induction of IFN-stimulated genes and lambda IFNs may limit the growth and spread of HCV in primary cell cultures and in the infected liver PMID: 21800339
  58. Expression levels of IL-29, IL-8, and cyclooxygenase (COX)-2 are elevated in patients with hepatitis B virus (HBV) infection and in HBV-transfected hepatoma cells. PMID: 21957142
  59. We investigated the regulation of IFN-lambda1 expression in human airway epithelial cells PMID: 22058416
  60. These data indicate that IFN-lambda, but not IFN-beta/alpha, contributes to the main first line defense via RIG-I-dependent pathway against respiratory virus infection in human nasal epithelial cells. PMID: 21816185
  61. IL-29/IL-28A suppress herpes simplex virus type 1 (HSV-1) infection of human NT2-N neurons. PMID: 21499846
  62. Patients with acute hepatitis C showed IL-29 levels intermediate between chronic hepatitis C and normal controls; and IL-29 serum levels were higher in patients who spontaneously resolved hepatitis C than in those who became chronic PMID: 21145813
  63. Data show that Ku70 mediates type III IFN induction by DNA. PMID: 21398614
  64. In contrast to IL-29, IL-28A is a potent gene repressor in patients with hepatitis C PMID: 21170333
  65. Interleukin-29 is involved in the pathogenesis of allergic inflammation via modulating immune cells' function to release proinflammatory cytokines. PMID: 20726961
  66. IL-29 up-regulated, whereas IFNalpha down-regulated, the surface expression of the IFNgamma receptor 1 chain on macrophages, thereby resulting in differential responsiveness of TLR-challenged macrophages to IFNgamma. PMID: 21190998
  67. These findings indicate that the expression of MxA, 2',5'-OAS and PKR are up-regulate by PI3K-AKT signal pathway, and Raf-MEK-ERK signal pathway has a negative regulatory effect on the expression of MxA and no significant effect on 2',5'-OAS and PKR. PMID: 20309637
  68. our data provide evidence for a critical role for the activated RSK1 in IFNlambda signaling PMID: 21075852
  69. Extrinsic IL-29 provoked IL-4 and IL-13 release from mast cell line P815 cells through PI3K/Akt and (JAK)/STAT3 signaling pathways, but failed to induce mast cell histamine release from human mast cells. PMID: 20337614
  70. The data indicates that interferons contribute to TLR-dependent gene activation in human dendritic cells stimulated with multiple TLR ligands. PMID: 21040977
  71. The overall structure of IFN-lambda1 is topologically similar to the structure of IL-10 and other members of the IL-10 family of cytokines. PMID: 20934432
  72. Interleukin-29 binds to melanoma cells inducing Jak-STAT signal transduction and apoptosis PMID: 20103601
  73. These data show that Hantaan virus can cause type I interferon-independent interferon-lambda1 induction and interferon-lambda1-specific gene induction. PMID: 20592090
  74. Although the IFN-lambda1(Bac)-IFN-lambda1R1 and IFN-lambda1(Ins)-IFN-lambda1R1 complexes differed only in the nature of the expression system used for the ligand, their crystallization conditions and crystal forms were quite different PMID: 20057073
  75. study supports that IFN-lambdas do not influence every type of cell and that membrane-associated variant IFN-lambda R1 expression is not sufficient to ensure cellular sensitivity toward these cytokines. PMID: 19798076
  76. identified from the human genomic sequence a family of three cytokines, designated interleukin 28A (IL-28A), IL-28B and IL-29, that are distantly related to type I interferons (IFNs) and the IL-10 family PMID: 12469119
  77. closely positioned genes on human chromosome 19 encode distinct but paralogous proteins, which we designate interferon-lambda1 (IFN-lambda1), IFN-lambda2 and IFN-lambda3 PMID: 12483210
  78. IL-29 antiviral and antiproliferative activity requires interleukin (IL)-28 receptor tyrosine residues PMID: 15166220
  79. IL-28A and IL-29 induced mRNA expression of the antiviral proteins 2',5'-OAS and MxA was abolished by overexpression of SOCS-1 PMID: 15850793
  80. we found IL-28A and IL-29 act similarly to IFNs, but are less effective generally and have activity in a more limited range of cell lines PMID: 15899585
  81. IECs express functional receptors for IFN-lambdas, which mediate antiviral and antiproliferative signals in IECs, suggesting a potential for therapeutic use in certain viral infections and as (antiproliferative) anticancer therapy PMID: 16051921
  82. IFN-lambdas are thus able to generate tolerogenic DCs, an activity that could thwart IFN-beta functions PMID: 16478884
  83. Study shows that IFNL1, a cytokine produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and may be important in activating innate immune responses at the site of viral infection. PMID: 17082759
  84. Interferon lambda-1 (IFN-lambda1/IL-29) induces ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells, in an IFN-gamma-independent manner. PMID: 17252004
  85. IFNs lambda exhibit several common features with type I IFNs: antiviral activity, antiproliferative activity and in vivo antitumour activity [review] PMID: 17367910
  86. Activation of toll-like receptors 2 and 3 show keratinocytes in the simultaneous presence of IL-20 and IL-29. PMID: 18281438
  87. the duration of IFNlambda signaling to JAK/STAT is different from that of IFNalpha PMID: 18698163
  88. Interleukin-29 functions cooperatively with interferon to induce antiviral gene expression and inhibit hepatitis C virus replication PMID: 18757365
  89. IFN-lambda1 may regulate myeloma cell biology PMID: 18830264
  90. IL-29 exerts IFN-beta-independent protection in alveolar type II cells through direct activation of antiviral genes during influenza A virus infection. PMID: 19155475
  91. These data provide direct and compelling evidence that IFN-lambda, through both extracellular and intracellular antiviral mechanisms, inhibits HIV-1 replication in macrophages. PMID: 19193806
  92. recent findings about the biology of IFN-lambdas and their pathophysiological roles in viral infection, cancer, and immune responses of the innate and adaptive arms.[review] PMID: 19304895
  93. IFN-lambda1-treated pDC display a marked difference in their ability to stimulate production of the signature cytokines IL-13, IFN-gamma, and IL-10 in a MLR PMID: 19759281

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Subcellular Location Secreted
Protein Families Lambda interferon family
Database Links

HGNC: 18363

OMIM: 607403

KEGG: hsa:282618

STRING: 9606.ENSP00000329991

UniGene: Hs.406745

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