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Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

Citations (1) Protocol MSDS
Code CSB-E15766m
Size 96T,5×96T,10×96T
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Product Details

Target Name
Monocyte Chemotactic Protein 5 (MCP-5)
Alternative Names
C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
Abbreviation
MCP-5
Uniprot No.
Q62401
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
16.82 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 87    
Range % 82-91    
1:2 Average % 94    
Range % 89-98    
1:4 Average % 107    
Range % 103-110    
1:8 Average % 110    
Range % 104-114    
Recovery
The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 91-99    
EDTA plasma (n=4) 103 98-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 2.615 2.634 2.625 2.499    
500 2.177 2.012 2.095 1.969    
250 1.432 1.502 1.467 1.341    
125 0.842 0.839 0.841 0.715    
62.5 0.531 0.529 0.530 0.404    
31.2 0.334 0.326 0.330 0.204    
15.6 0.246 0.251 0.249 0.123    
0 0.125 0.127 0.126 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse MCP-5 ELISA Kit was designed for the quantitative measurement of Mouse MCP-5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 16.82 pg/mL.

Citations

Related Products

Ccl12 antibodies

Ccl12 Proteins

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Target Background

Function
(From Uniprot)
Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
Gene References into Functions
  1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145
  2. Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370
  3. The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
  4. CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
Database Links

KEGG: mmu:20293

STRING: 10090.ENSMUSP00000000194

UniGene: Mm.867

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The Overview of Chemokine ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments
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