Recombinant Mouse C-C motif chemokine 12 protein(Ccl12) (Active)

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Code	CSB-AP001301MO
Size	US$3274Purchase it in Cusabio online store (only available for customers from the US)
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Product Details
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Target Data

Product Details

Purity	>95% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
Target Names	Ccl12
Uniprot No.	Q62401
Research Area	Immunology
Alternative Names	C-C motif chemokine 12; Ccl12; CCL12_MOUSE; Macrophage/monocyte Chemotactic Protein; MCP-1-related chemokine; MCP-5; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; OTTMUSP00000000792; Scya12; Small inducible cytokine A12; Small-inducible cytokine A12
Species	Mus musculus (Mouse)
Source	E.coli
Expression Region	23-104aa
Complete Sequence	GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
Mol. Weight	9.3 kDa
Protein Length	Full Length of Mature Protein
Tag Info	Tag-Free
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Data

Function	Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
Gene References into Functions	After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145 Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370 The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601 CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
Subcellular Location	Secreted
Protein Families	Intercrine beta (chemokine CC) family
Tissue Specificity	Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
Database Links	KEGG: mmu:20293 STRING: 10090.ENSMUSP00000000194 UniGene: Mm.867