Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells is typically 1-10 ng/mL.

Target Names

CXCL14

Uniprot No.

O95715

Research Area

Immunology

Alternative Names

1110031L23Rik; 1200006I23Rik; AI414372; BMAC; bolekine; BRAK; Breast and kidney; C-X-C motif chemokine 14; C-X-C motif chemokine ligand 14; Chaemokine; CXC motif; ligand 14; Chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXC chemokine in breast and kidney ; CXCL14; CXL14_HUMAN; JSC; Kec; Kidney-expressed chemokine CXC; KS1; MGC10687; MGC124510; MGC90667; MIP 2 gamma; MIP-2G; MIP2G; MIP2gamma; NJAC; PRO273; PSEC0212; Scyb14; Small Inducible Cytokine B14; Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK); Small Inducible Cytokine subfamily B; member 14; Small-inducible cytokine B14; Tumor suppressing chemokine; UNQ240

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

35-111aa

Complete Sequence

SKCKCSRKGPKIRYSDVKKLEMKPKYPHCEEKMVIITTKSVSRYRGQEHCLHPKLQSTKRFIKWYNAWNEKRRVYEE

Mol. Weight

9.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 1 M NaCl, pH 8.5

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Introducing our Recombinant Human CXCL14 protein, a crucial tool for researchers in the field of immunology. This C-X-C motif chemokine 14, known as CXCL14, MIP2G, NJAC, and SCYB14, is expressed in E. coli and covers the 35-111aa expression region of the full-length mature protein. The tag-free protein is provided as a lyophilized powder for straightforward reconstitution with sterile water or buffer, ensuring a variety of experimental applications.

Quality and performance are our top priorities, and our Recombinant Human CXCL14 protein displays a purity of >95% as determined by SDS-PAGE and HPLC analysis. Additionally, endotoxin levels are controlled below 1.0 EU/µg, as determined by the LAL method. The protein exhibits biological activity as assessed by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells, with an ED₅₀ typically in the range of 1-10 ng/mL.

CXCL14 has been widely investigated for its roles in immune regulation, tumor progression, and cancer^[1]. Studies have revealed its chemotactic functions on monocytes and dendritic cells^[2] and its tumor-suppressive properties in several cancer types^[3]. This highlights the significance of CXCL14 in immune system research and its potential as a therapeutic target for immune-related diseases and cancer.

References:
1. Hara T, et al. Identification of a chemotactic ligand for CXCR1 and CXCR2 in human osteosarcoma cells. Biochem Biophys Res Commun. 2009;386(4): 694-9.
2. Hromas R, et al. Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells. Biochem Biophys Res Commun. 1999;255(3): 703-6.
3. Tanegashima K, et al. The Chemokine CXCL14 suppresses the tumorigenicity of hepatocellular carcinoma cells. Int J Cancer. 2013;132(4): 949-60.

Target Background

Function

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.

Gene References into Functions

These findings indicate that CXCL14 is a critical immunomodulator involved in the stroke-induced inflammatory reaction. PMID: 28382159
High CXCL14 expression is associated with metastatic progression of Ovarian Cancer. PMID: 28087599
These results suggest that CXCL14 downregulation by human papillomaviruses plays an important role in suppression of antitumor immune responses. PMID: 27143385
Platelets are a relevant source of CXCL14. Platelet-derived CXCL14 at the site of vascular lesions might play an important role in vascular repair/regeneration. PMID: 28359053
Epithelial CXCL14 expression is significantly associated with ERalpha positivity and low proliferation, whereas stromal CXCL14 expression is not linked to any of the established clinicopathological parameters, subtypes of breast cancer or tumour stroma abundance. PMID: 27115465
Elevated expression of CXCL14 in osteosarcoma tissues correlated with poor prognosis of the osteosarcoma patients. PMID: 27259322
Elevated S100A6 enhances tumorigenesis and suppresses CXCL14-induced apoptosis in clear cell renal cell carcinoma. PMID: 25760073
three of these five genes (CXCL14, ITGAX, and LPCAT2) harbored polymorphisms associated with aggressive disease development in a human GWAS cohort consisting of 1,172 prostate cancer patients. PMID: 25411967
Prometastatic effects of IRX1 were mediated by upregulation of CXCL14/NF-kappaB signaling. PMID: 25822025
CXCL14 overexpression influences proliferation and changes in cell cycle distributions of HT29 colorectal carcinoma cells. PMID: 24938992
Data indicate that site-specific CpG methylation in the CXC chemokine CXCL14 promoter is associated with altered expression. PMID: 25102097
CXCL14 displays antimicrobial activity against E. coli and S. aureus. PMID: 12949249
Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts. PMID: 24710408
CXCL14 inhibits colorectal cancer migration, invasion, and epithelial-to-mesenchymal transition (EMT) by suppressing NF-kappaB signaling. PMID: 24099668
Downregulation of CXCL14 expression is associated with gastric adenocarcinoma. PMID: 23982764
CXCL14 plays a pivotal role as a potential tumor suppressor in hepatocellular carcinoma. PMID: 24033560
Smoking-induced CXCL14 expression in the human airway epithelium links chronic obstructive pulmonary disease to lung cancer. PMID: 23597004
CXCL14 binding to glycoproteins harboring heparan sulfate proteoglycans and sialic acids leads proliferation and migration of some cancer cells. PMID: 23161284
CXCL14 might be a potential novel prognostic factor to predict the disease recurrence and overall survival and could be a potential target of postoperative adjuvant therapy in CRC patients PMID: 23294544
CXCL14 is a negative regulator of growth and metastasis in breast cancer. PMID: 22910931
These results indicated that upregulation of BRAK was accompanied by differentiation of epithelial cells induced by calcium/calmodulin signaling, and that SP1 binding to the BRAK promoter region played an important role in this signaling. PMID: 22382027
The rs2237062 polymorphism in the CXCL14 gene might influence Hepatits B Virus-related hepatocellular carcinoma progression in a Chinese population. PMID: 21556757
CEACAM-1 and CXCL-14 are involved in the occurrence and development of infantile hemangioma. PMID: 20737948
The results indicate that oxidative stress induced by H(2)O(2) or HO(*) stimulates angiogenesis and tumuor progression by altering the gene expression of CXCL14 via the EGFR/MEK/ERK pathway in human HNSCC cells. PMID: 20815772
Data indicate that the expression of BRAK stimulated the formation of elongated focal adhesions of the HSC-3 cells in an autocrine or paracrine fashion, in which stimulation may be responsible for the reduced migration of the cells. PMID: 20067447
CXCL14 methylation in sputum from asymptomatic early-stage lung cancer cases was associated with a 2.9-fold elevated risk for this disease compared with controls, substantiating its potential as a biomarker for early detection of lung cancer PMID: 20562917
Taken together, the data indicate that the respective stress-dependent action of p38 isoforms is responsible for the up-regulation of the gene expression of the chemokine BRAK/CXCL14. PMID: 20478268
CXCL14 removal from conditioned media abolished its chemotactic properties. Findings offer direct evidence for epigenetic regulation of chemokine expression in tumor cells. PMID: 20460540
Increased severity of collagen-induced arthritis in CXCL14-transgenic mice is associated with enhanced T helper (Th) type 1 cytokine production, elevated autoantibody levels and increased inflammatory cell infiltration into the joints. PMID: 20212097
Data conclude that CXCL14 is likely to be regulated by progesterone in human endometrium and that it may exert a chemoattractive effect on uNK cells and in part be responsible for their clustering around the epithelial glands. PMID: 19903701
Results suggest that CXCL14 plays an important role in regulating trophoblast invasion through an autocrine/paracrine manner during early pregnancy. PMID: 19833716
loss of BRAK expression from tumors may facilitate neovascularization and possibly contributes to immunologic escape PMID: 15548693
The finding that CXCL14 expression inhibits prostate tumor growth suggests this gene has tumor suppressive functions. PMID: 15651028
CXCL14 is a potent chemoattractant and activator of dendritic cells (DC) and may be involved in DC homing in vivo. PMID: 15843547
results indicate that BRAK/CXCL14 is a chemokine, having suppressive activity toward tumor progression of oral carcinoma in vivo PMID: 16884687
This study elucidates a post-translational mechanism for the loss of CXCL14 in cancer and a novel mode of chemokine regulation. PMID: 16987528
CXCL14 might play a pivotal role in the pathobiology of pancreatic cancer, probably by regulating cancer invasion. PMID: 18054154
CXCL14 is a gene target of RhoBTB2 and supports downregulation of CXCL14 as a functional outcome of RhoBTB2 loss in cancer. PMID: 18762809
CXCL14-positive epithelial cells were found in all tissue types. The expression of CXCL14 was not associated with any tumor or patient characteristics analyzed PMID: 18765527
Data suggest that despite the structural homology and similarity in tissue distribution of human and murine CXCL14, distinct differences point to diverse, species-specific needs for CXCL14 in epithelial immunity. PMID: 18809336
Cell supernatant-derived CXCL14 fights bacteria at the earliest stage of infection, well before the establishment of inflammation, and thus fulfills a unique role in antimicrobial immunity. PMID: 19109182
regulates energy metabolism and eating behaviior, induces insulin resistance, suppresses induction of neovascularization. (review) PMID: 19172796
identify CXCL14 as a novel autocrine stimulator of fibroblast growth and migration, with multi-modal tumor-stimulatory activities PMID: 19218429
CXCL14 expression is upregulated by ROS through the AP-1 signaling pathway and promotes cell motility through elevation of cytosolic Ca(2+) by binding to the inositol 1,4,5-trisphosphate receptor on the endoplasmic reticulum in breast cancer. PMID: 19276362
-A-induced migration depends on the selective and polarized release of 2 chemokines, namely CXC chemokine ligands 12 and 14 PMID: 19339694

Hide All

Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. N

Database Links

HGNC: 10640

OMIM: 604186

KEGG: hsa:9547

STRING: 9606.ENSP00000337065

UniGene: Hs.483444

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Code	CSB-AP003521HU
Size	US$153
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human C-X-C motif chemokine 14 (CXCL14) (Active)

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