Product Details

Purity

>98% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/ml.

Target Names

CXCL6

Uniprot No.

P80162

Research Area

Immunology

Alternative Names

C X C motif chemokine 6; Chemokine (C X C motif) ligand 6; Chemokine alpha 3; CKA 3; CKA-3; CKA3; CXCL 6; CXCL6; CXCL6_HUMAN; GCP 2; GCP-2; Granulocyte chemotactic protein 2; N-processed variant 3; SCYB6; Small inducible cytokine B6; Small inducible cytokine subfamily B (Cys X Cys) member 6; Small inducible cytokine subfamily B (Cys X Cys) member b; Small-inducible cytokine B6

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

43-114aa

Complete Sequence

VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN

Mol. Weight

7.9 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CXCL6 (GCP-2) is a potent ELR+ CXC chemokine that signals through CXCR1 and CXCR2 to drive neutrophil recruitment, making it a critical tool for dissecting innate immune trafficking mechanisms. This tag-free recombinant human CXCL6, spanning residues 43–114 of the mature processed sequence, demonstrates robust chemotactic activity on primary human neutrophils at 10–50 ng/ml, providing a suitable basis for Transwell migration assays, CXCR1/CXCR2 signaling pathway studies involving MAPK and NF-κB activation, and in vivo neutrophilic inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound neutrophil priming and cytokine release readouts, which satisfies the stringent criteria typical for primary immune cell functional assays. Produced in *E. coli* with greater than 98% purity by SDS-PAGE, this recombinant CXCL6 protein also supports use as a reference standard in chemokine detection assays and as a coating antigen for anti-CXCL6 antibody validation by ELISA.

Target Background

Function

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

Gene References into Functions

CXCL6 could reduce the expression of miR-515-5p in NSCLC cells; MiR-515-5p acted as a tumor suppressor by targeting CXCL6 in non-small cell lung cancer cell cells. PMID: 29136957
Study has provided the first report of fibroblast-derived CXCL12 enhancement of CXCL6 secretion in colon cancer cells, and of both CXCL12 and CXCL6 co-operatively regulating metastasis through the PI3K/Akt/mTOR signaling pathway. PMID: 28811711
CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
CXCL6 level is high in the serum of chronic hepatitis B patients.CXCL6 promotes human hepatocyte proliferation through the CXCR1-NFkappaB pathway and inhibits collagen I secretion by hepatic stellate cells. PMID: 27032929
The role of PITX2 in glaucoma may be mediated partly by regulating the expression of CXCL6 and BBS5 and thus affecting immune functions and intraocular pressure. PMID: 27520585
The neutrophil-recruiting chemokine GCP-2/CXCL6 is expressed in cystic fibrosis airways and retains its functional properties after binding to extracellular DNA. PMID: 25993443
HIF-1alpha promotes HCC progression and metastasis by upregulating CXCL6 transcription in HCC cells. PMID: 25323032
Expression of the CXCL8, CXCL6 and CXCL1 genes are under the primary control of 1,25-dihydroxyvitamin D3 and its receptor. PMID: 24250750
Data suggest that expression of CXCL6 in trophoblasts is up-regulated during pregnancy development/placentation; CXCL6 expression inhibits trophoblast cell migration and invasion by down-regulating activity of MMP2 (matrix metalloproteinase 2). PMID: 23814098
Overexpression of GCP-2 in mesenchymal stem cells has the potential to enhance their angiogenic and survival properties. PMID: 22886775
Studies show that functional blocking of GCP-2 inhibits tumor growth and metastases. PMID: 21236563
The aim of this study was to investigate the role of the transcription factors, AP-1 and NF-kappaB, in IL-6 and CXCL8 regulation in Jurkat T-cells. PMID: 20507572
The level of expression of granulocyte chemotactic protein 2 correlates with the severity of periodontitis and appears to act as a yet unrecognized functional adjunct to interleukin-8 in pathological gingival tissues. PMID: 18842116
results suggest that granulocyte chemotactic protein-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells PMID: 12524079
induction in mesenchymal cells by interleukin-1beta and down-regulation by interferon-gamma PMID: 12533683
Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP-2 (CXCL6). PMID: 12628493
expression in inflamed intestinal tissue in Crohn's disease PMID: 15214047
production of GCP-2 by endothelial cells within the tumor can contribute to tumor development through neovascularization due to endothelial cell chemotaxis and to tumor cell invasion and metastasis PMID: 15652347
LDL lipoprotein subunit L5 induces human umbilical vein endothelial cells (HUVEC) to express CXCL6. PMID: 17022986
These findings indicate that the equilibrium between angiostatic and angiogenic factors during inflammation and tumor progression is rather complex and differs depending on the chemokine, cell type, and stimulus. PMID: 17827342
These observations suggest a role for CXCL6 in the innate immune response to microbial invasion of the amniotic cavity. PMID: 18782286
Report gonadotropin-releasing hormone-regulated CXCL6 expression in human placentation. PMID: 19369450

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

HGNC: 10643

OMIM: 138965

KEGG: hsa:6372

STRING: 9606.ENSP00000226317

UniGene: Hs.164021

Code	CSB-AP000691HU
Abbreviation	Recombinant Human CXCL6 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human C-X-C motif chemokine 6 protein (CXCL6), partial (Active)

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