Product Details

Target Name

chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)

Alternative Names

C X C motif chemokine 6 ELISA Kit; Chemokine (C X C motif) ligand 6 ELISA Kit; Chemokine alpha 3 ELISA Kit; CKA 3 ELISA Kit; CKA-3 ELISA Kit; CKA3 ELISA Kit; CXCL 6 ELISA Kit; CXCL6 ELISA Kit; CXCL6_HUMAN ELISA Kit; GCP 2 ELISA Kit; GCP-2 ELISA Kit; Granulocyte chemotactic protein 2 ELISA Kit; N-processed variant 3 ELISA Kit; SCYB6 ELISA Kit; Small inducible cytokine B6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member b ELISA Kit; Small-inducible cytokine B6 ELISA Kit

Abbreviation

CXCL6

Uniprot No.

P80162

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	624.092	617.035	632.238	637.984	631.667	633.154
SD	0.032	0.040	0.049	0.051	0.055	0.060
CV(%)	3.683	5.398	6.274	6.544	6.294	6.762

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GCP-2/CXCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

81-88

1:2

Average %

Range %

89-96

1:4

Average %

102

Range %

98-105

1:8

Average %

Range %

95-104

Recovery

The recovery of human GCP-2/CXCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	100	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



pg/ml	OD1	OD2	Average	Corrected
5000	2.750	2.842	2.796	2.658
2500	1.947	2.055	2.001	1.863
1250	1.201	1.280	1.241	1.103
625	0.763	0.798	0.781	0.643
312	0.461	0.433	0.447	0.309
156	0.286	0.293	0.290	0.152
78	0.233	0.216	0.225	0.087
0	0.170	0.180	0.175

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human GCP-2 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human GCP-2 in serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the GCP-2 levels in the sample and is measured at 450 nm through a microplate reader.

GCP-2, also known as CXCL6, is a neutrophil-recruiting ELR+ chemokine that signals through the receptors CXCR1 and CXCR2 that are expressed on neutrophils. CXCL6-CXCR1/2 signaling mediates multiple biological processes such as inflammation, immunoreaction, cell growth, and metastasis in humans. It also promotes human hepatocyte proliferation through the CXCR1-NF-κB pathway and suppresses collagen I secretion by hepatic stellate cells. CXCL6 is involved in cancer angiogenesis, metastasis, and immune response. CXCL6 promotes the growth and metastasis of various cancers, including non-small cell lung cancer and osteosarcoma (OS).

Target Background

Function
(From Uniprot)

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

Gene References into Functions

CXCL6 could reduce the expression of miR-515-5p in NSCLC cells; MiR-515-5p acted as a tumor suppressor by targeting CXCL6 in non-small cell lung cancer cell cells. PMID: 29136957
Study has provided the first report of fibroblast-derived CXCL12 enhancement of CXCL6 secretion in colon cancer cells, and of both CXCL12 and CXCL6 co-operatively regulating metastasis through the PI3K/Akt/mTOR signaling pathway. PMID: 28811711
CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
CXCL6 level is high in the serum of chronic hepatitis B patients.CXCL6 promotes human hepatocyte proliferation through the CXCR1-NFkappaB pathway and inhibits collagen I secretion by hepatic stellate cells. PMID: 27032929
The role of PITX2 in glaucoma may be mediated partly by regulating the expression of CXCL6 and BBS5 and thus affecting immune functions and intraocular pressure. PMID: 27520585
The neutrophil-recruiting chemokine GCP-2/CXCL6 is expressed in cystic fibrosis airways and retains its functional properties after binding to extracellular DNA. PMID: 25993443
HIF-1alpha promotes HCC progression and metastasis by upregulating CXCL6 transcription in HCC cells. PMID: 25323032
Expression of the CXCL8, CXCL6 and CXCL1 genes are under the primary control of 1,25-dihydroxyvitamin D3 and its receptor. PMID: 24250750
Data suggest that expression of CXCL6 in trophoblasts is up-regulated during pregnancy development/placentation; CXCL6 expression inhibits trophoblast cell migration and invasion by down-regulating activity of MMP2 (matrix metalloproteinase 2). PMID: 23814098
Overexpression of GCP-2 in mesenchymal stem cells has the potential to enhance their angiogenic and survival properties. PMID: 22886775
Studies show that functional blocking of GCP-2 inhibits tumor growth and metastases. PMID: 21236563
The aim of this study was to investigate the role of the transcription factors, AP-1 and NF-kappaB, in IL-6 and CXCL8 regulation in Jurkat T-cells. PMID: 20507572
The level of expression of granulocyte chemotactic protein 2 correlates with the severity of periodontitis and appears to act as a yet unrecognized functional adjunct to interleukin-8 in pathological gingival tissues. PMID: 18842116
results suggest that granulocyte chemotactic protein-2 is an additional ELR(+)-CXC chemokine expressed in endometrial stromal cells PMID: 12524079
induction in mesenchymal cells by interleukin-1beta and down-regulation by interferon-gamma PMID: 12533683
Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP-2 (CXCL6). PMID: 12628493
expression in inflamed intestinal tissue in Crohn's disease PMID: 15214047
production of GCP-2 by endothelial cells within the tumor can contribute to tumor development through neovascularization due to endothelial cell chemotaxis and to tumor cell invasion and metastasis PMID: 15652347
LDL lipoprotein subunit L5 induces human umbilical vein endothelial cells (HUVEC) to express CXCL6. PMID: 17022986
These findings indicate that the equilibrium between angiostatic and angiogenic factors during inflammation and tumor progression is rather complex and differs depending on the chemokine, cell type, and stimulus. PMID: 17827342
These observations suggest a role for CXCL6 in the innate immune response to microbial invasion of the amniotic cavity. PMID: 18782286
Report gonadotropin-releasing hormone-regulated CXCL6 expression in human placentation. PMID: 19369450

Hide All

Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

HGNC: 10643

OMIM: 138965

KEGG: hsa:6372

STRING: 9606.ENSP00000226317

UniGene: Hs.164021

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Code	CSB-E09990h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Human granulocyte chemotactic protein-2,GCP-2 ELISA Kit

Product Details

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Target Background