Product Details

Purity

>96% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.

Target Names

Ccl11

Uniprot No.

P48298

Research Area

Immunology

Alternative Names

Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

24-97aa

Complete Sequence

HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

Mol. Weight

8.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL11 (Eotaxin) is a potent CC chemokine that selectively recruits eosinophils via CCR3 signaling, making it a critical tool for dissecting allergic inflammation and type 2 immune responses. This tag-free recombinant mouse Eotaxin, expressed in *E. coli* as the full-length mature protein (residues 24–97), demonstrates confirmed biological activity in eosinophil chemotaxis bioassays at 100–1000 ng/ml, supporting use in transwell migration assays, CCR3 receptor activation studies, and MAPK/calcium flux signaling experiments. The faithful mature sequence and absence of fusion tags provide a suitable basis for use as an immunogen in antibody development, as a positive control in ELISA-based cytokine detection, or as a reference standard for quantifying endogenous Eotaxin levels. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive primary eosinophil and mast cell assays, while purity exceeding 96% by SDS-PAGE aligns with standards expected in murine models of airway inflammation and allergic disease.

Target Background

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.

Gene References into Functions

the adipose-derived FGF21-CCL11 axis triggers cold-induced beiging and thermogenesis by coupling sympathetic nervous system to activation of type 2 immunity in subcutaneous white adipose tissue. PMID: 28844880
CCL11 promotes migration and proliferation of mouse neural progenitor cells. PMID: 28173860
These studies characterized serum and intestinal wall eotaxin-1 levels in various inflammatory bowel disease patients and to explore the effect of targeting eotaxin-1 by specific antibodies in dextran sodium sulfate-induced colitis model. PMID: 26874691
this study shows that eosinophil trafficking to the heart is dependent on the eotaxin-CCR3 pathway in a mouse model of experimental autoimmune myocarditis PMID: 27621211
Blocking antibodies against RANTES and eotaxin reduced the infiltration of CD4(+) and CD8(+) T cells into the nigra, attenuated nigral expression of proinflammatory molecules, and suppressed nigral activation of glial cells. These findings paralleled dopaminergic neuronal protection, normalized striatal neurotransmitters, and improved motor functions in MPTP-intoxicated mice. PMID: 27226559
These results indicate that CCL11 was responsible for the limited angiogenesis and necrosis by inducing and attracting eosinophils in the tumors. PMID: 27169545
Study demonstrated that CCL11 is primarily produced by activated astrocytes in the CNS, activates microglia to produce ROS via NOX1, and exacerbates excitotoxic neuronal death PMID: 26184677
PAR2 activation through endogenous mast cell tryptase activity could be required, at least partially, to mediate CCL11-induced eosinophil migration PMID: 24972241
The chemokines monocyte chemotactic protein 1 (MCP1), MIP1alpha, MIP1beta, interferon gamma-induced protein 10 (IP-10), and eotaxin were induced in Saa1 TG mice. PMID: 25847238
investigated role of Eotaxin-1 on disease outcome in Litomosoides sigmodontis infection; findings suggest, in Eotaxin-1(-/-) mice, potential reduced activation state of eosinophils; macrophages produce decreased amounts of IL-6 in vitro suggesting possible mechanisms by which Eotaxin-1 regulates activation of inflammatory cells and parasite survival PMID: 24112106
Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
TNC expression controls eotaxin level in apo E-/- mice and that this chemokine plays a key role in the development of atherosclerosis PMID: 23433402
Data indicate that the combination of Ovalbumin (OVA) and hypoxia induced a enhanced expression of HIF-1alpha and increased eotaxin-1, lung TGB-beta1 expression, and indices of airway remodeling. PMID: 23499929
increased sputum and nasal lavage fluid levels in allergic rhinitis subjects PMID: 21703102
These studies demonstrate that inflammatory monocyte/macrophage-derived CCL11 drives colonic eosinophilic inflammation in experimental colitis. PMID: 21498668
The oesophageal production of CCL11 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
Data show that that augmented airway eosinophilic inflammation and hyperresponsiveness in RV-infected mice with allergic airways disease is directed in part by eotaxin-1. PMID: 20644177
eotaxin initiates allergic airway disease due to A. fumigatus, but this chemokine did not appear to contribute to the maintenance of A. fumigatus-induced allergic airway disease. PMID: 12060577
concentrations of eotaxin in the CSF of Angiostrongylus cantonensis infected mice each week after infection were all significantly higher than those in serum ( P<0.0001). PMID: 14648203
CCL11 is a potent chemotactic factor for smooth muscle cells. Because CCL11 is expressed abundantly in SMC-rich areas of the atherosclerotic plaque and in injured arteries, it may play an important role in regulating SMC migration. PMID: 15130922
infection of mice by Histoplasma capsulatum induced rapid generation of high levels of MIP-1alpha, which remained elevated from 4-48 h whereas very little eotaxin was detected at any time point PMID: 15316665
critical role for eotaxin-1 in Brugia malayi microfilaria parasite clearance PMID: 15593125
Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
The eotaxin-1 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
CCL11 and CCR3 are important in the pulmonary recruitment of granulocytes and play significant pathogenic roles in bleomycin-induced lung fibrosis. PMID: 16314464
IL-4 induction and the IL-4/OSM synergistic induction of eotaxin-1 was abrogated in STAT6(-/-) mouse lung fibroblasts, however, regulation of IL-6 was similar in -/- or wild-type mouse lung fibroblasts PMID: 16547273
CCL11 is the salient but not the sole eosinophil chemoattractant of biological significance during gastrointestinal helminth infection. PMID: 16783848
eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
In the absence of eotaxin-2 or CCR3, there was a profound reduction in IL-13-induced eosinophil recruitment into the lung lumen. In the absence of eotaxin-1, there was a fourfold increase in IL-13-mediated eosinophil recruitment into the airway. PMID: 17148674
There was a higher tumor incidence in CCL11(-/-) BALB/c mice, which was associated with a reduced eosinophil influx into tumors. PMID: 17371978
Heligmosomoides infection led to reduction in number of lung eosinophils with decreased levels of eotaxin in bronchoalveolar lavage fluid, lowered of CCR3 receptor expression on eosinophils and impaired chemotaxis of these cells toward eotaxin. PMID: 17650182
induction of eotaxin-1 and CD4+ T cell production of IL-5 are required for respiratory syncytial virus G glycoprotein-induced eosinophilia following respiratory syncytial virus challenge. PMID: 18519743
Specific inhibition of CCL11 alone is therefore unlikely to inhibit eosinophil recruitment to the airways. PMID: 18850374
Data show thatprimary or secondary resistance were unaffected at either the pre-lung or gut stages of infection in eotaxin(-/-) single mutant mice. PMID: 19535141

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Database Links

KEGG: mmu:20292

STRING: 10090.ENSMUSP00000000342

UniGene: Mm.4686

Code	CSB-AP001291MO
Abbreviation	Recombinant Mouse Ccl11 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse Eotaxin protein (Ccl11) (Active)

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Target Background

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